LOCUS       40924_7131        5005 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBS-35S-Rluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Subramanian C, Kim BH, Lyssenko NN, Xu X, Johnson CH, von Arnim AG.
  TITLE     The Arabidopsis repressor of light signaling, COP1, is regulated by 
            nuclear exclusion: mutational analysis by bioluminescence resonance 
            energy transfer
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 101 (17), 6798-6802 (2004)
  PUBMED    15084749
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   von Arnim A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2002) Botany, University of Tennessee, 1406 Circle
            Drive, Knoxville, TN 37996, USA
REFERENCE   3  (bases 1 to 5005)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5005)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2004"; volume: "101"; issue: "17"; pages:
            "6798-6802"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-DEC-2002) Botany, University of Tennessee, 1406 Circle Drive, 
            Knoxville, TN 37996, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        789..1460
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1460..1602
                     /note="translational enhancer; 5'UTR from tobacco etch
                     virus"
     CDS             1603..2535
                     /label=hRluc
                     /note="Renilla luciferase"
     regulatory      2548..2758
                     /gene="RLuc"
                     /label=cauliflower mosaic virus 35S terminator
                     /note="cauliflower mosaic virus 35S terminator"
                     /regulatory_class="terminator"
     promoter        complement(2820..2838)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2859..2875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2883..2899)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2907..2937)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2952..2973)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3261..3849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4023..4880)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4881..4985)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gcccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca ggtcaacatg
      721 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
      781 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
      841 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
      901 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
      961 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1021 aagcaagtgg attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat
     1081 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
     1141 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
     1201 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
     1261 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     1321 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     1381 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     1441 tcatttcatt tggagaggac ctcgagaatt ctcaacacaa catatacaaa acaaacgaat
     1501 ctcaagcaat caagcattct acttctattg cagcaattta aatcatttct tttaaagcaa
     1561 aagcaatttt ctgaaaattt tcaccattta cgaacgatag ccatggcttc gaaagtttat
     1621 gatccagaac aaaggaaacg gatgataact ggtccgcagt ggtgggccag atgtaaacaa
     1681 atgaatgttc ttgattcatt tattaattat tatgattcag aaaaacatgc agaaaatgct
     1741 gttatttttt tacatggtaa cgcggcctct tcttatttat ggcgacatgt tgtgccacat
     1801 attgagccag tagcgcggtg tattatacca gaccttattg gtatgggcaa atcaggcaaa
     1861 tctggtaatg gttcttatag gttacttgat cattacaaat atcttactgc atggtttgaa
     1921 cttcttaatt taccaaagaa gatcattttt gtcggccatg attggggtgc ttgtttggca
     1981 tttcattata gctatgagca tcaagataag atcaaagcaa tagttcacgc tgaaagtgta
     2041 gtagatgtga ttgaatcatg ggatgaatgg cctgatattg aagaagatat tgcgttgatc
     2101 aaatctgaag aaggagaaaa aatggttttg gagaataact tcttcgtgga aaccatgttg
     2161 ccatcaaaaa tcatgagaaa gttagaacca gaagaatttg cagcatatct tgaaccattc
     2221 aaagagaaag gtgaagttcg tcgtccaaca ttatcatggc ctcgtgaaat cccgttagta
     2281 aaaggtggta aacctgacgt tgtacaaatt gttaggaatt ataatgctta tctacgtgca
     2341 agtgatgatt taccaaaaat gtttattgaa tcggacccag gattcttttc caatgctatt
     2401 gttgaaggtg ccaagaagtt tcctaatact gaatttgtca aagtaaaagg tcttcatttt
     2461 tcgcaagaag atgcacctga tgaaatggga aaatatatca aatcgttcgt tgagcgagtt
     2521 ctcaaaaatg aacaaagatc tatctagagt ccgcaaaaat caccagtctc tctctacaaa
     2581 tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta
     2641 gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg
     2701 tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacc
     2761 tgcagcccgg gggatccact agagcggccg ccaccgcggt ggagctccag cttttgttcc
     2821 ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga
     2881 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2941 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     3001 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     3061 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3241 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3661 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     3721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4021 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4081 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4141 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4201 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4261 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4321 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4381 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4441 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4501 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4561 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4621 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4681 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4741 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4801 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4861 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4921 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4981 ccgcgcacat ttccccgaaa agtgc
//
