pBrachyTAG-G227 vector (V009097)

LOCUS       V009097                 8418 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009097
VERSION     V009097
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Thole V, Worland B, Vain P.
  TITLE     pBrachyTAG vector system: New tools for T-DNA tagging and trapping
            in grasses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2011) Department of Crop Genetics, John Innes
            Centre, Norwich Research Park, Colney Lane, Norwich, Norfolk NR4
            7UH, United Kingdom
REFERENCE   3  (bases 1 to 8418)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8418)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-JUN-2011) Department of Crop Genetics, John Innes Centre,
            Norwich Research Park, Colney Lane, Norwich, Norfolk NR4 7UH, United
            Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(823..1635)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="spacer sequence separating left border No.1 and No.2
                     of the double left border sequence"
     misc_feature    2559..2583
                     /note="nopaline-type T-DNA left border No.2 belonging to a
                     double left border sequence separated by a spacer sequence
                     from left border No.1"
     misc_feature    2598..2693
                     /note="UniBox1; synthetic unique sequence containing
                     forward and reverse primer sequences"
     terminator      complement(2713..2965)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          complement(3693..3882)
                     /label="cat1 intron"
                     /note="castor bean catalase intron, modified"
     promoter        complement(4231..4575)
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      complement(4672..4964)
                     /note="SPA-ter; soybean-derived poly(A) signal"
                     /regulatory_class="terminator"
     misc_feature    4965..5106
                     /note="spacer sequence (containing non-useable ER targeting
                     signal sequences) to be replaced with gene-of-interest
                     using unique cloning sites KpnI (nt 4965-4970) and HindIII
                     (nt 5101-5106)"
     promoter        complement(5107..7103)
                     /label="Ubi promoter"
                     /note="maize polyubiquitin gene promoter"
     misc_feature    7153..7256
                     /note="UniBox2; synthetic unique sequence containing
                     forward and reverse primer sequences"
     misc_feature    7263..7287
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    7288..7311
                     /label="right border overdrive sequence"
                     /note="right border overdrive sequence"
     CDS             7515..8315
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"