LOCUS       V009097                 8418 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009097
VERSION     V009097
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Thole V, Worland B, Vain P.
  TITLE     pBrachyTAG vector system: New tools for T-DNA tagging and trapping
            in grasses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2011) Department of Crop Genetics, John Innes
            Centre, Norwich Research Park, Colney Lane, Norwich, Norfolk NR4
            7UH, United Kingdom
REFERENCE   3  (bases 1 to 8418)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8418)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-JUN-2011) Department of Crop Genetics, John Innes Centre,
            Norwich Research Park, Colney Lane, Norwich, Norfolk NR4 7UH, United
            Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(823..1635)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="spacer sequence separating left border No.1 and No.2
                     of the double left border sequence"
     misc_feature    2559..2583
                     /note="nopaline-type T-DNA left border No.2 belonging to a
                     double left border sequence separated by a spacer sequence
                     from left border No.1"
     misc_feature    2598..2693
                     /note="UniBox1; synthetic unique sequence containing
                     forward and reverse primer sequences"
     terminator      complement(2713..2965)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          complement(3693..3882)
                     /label="cat1 intron"
                     /note="castor bean catalase intron, modified"
     promoter        complement(4231..4575)
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      complement(4672..4964)
                     /note="SPA-ter; soybean-derived poly(A) signal"
                     /regulatory_class="terminator"
     misc_feature    4965..5106
                     /note="spacer sequence (containing non-useable ER targeting
                     signal sequences) to be replaced with gene-of-interest
                     using unique cloning sites KpnI (nt 4965-4970) and HindIII
                     (nt 5101-5106)"
     promoter        complement(5107..7103)
                     /label="Ubi promoter"
                     /note="maize polyubiquitin gene promoter"
     misc_feature    7153..7256
                     /note="UniBox2; synthetic unique sequence containing
                     forward and reverse primer sequences"
     misc_feature    7263..7287
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    7288..7311
                     /label="right border overdrive sequence"
                     /note="right border overdrive sequence"
     CDS             7515..8315
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1441 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     2221 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     2461 ggatttccga tccccggaat tagatccgtt taaactacgt aagatcttgg caggatatat
     2521 tgtggtgtaa acgttcctgc ggcggtcgag atggatcttg gcaggatata ttgtggtgta
     2581 aacgttcctg cggccgctac gttagatcgt attctacagc ttccgtatca gccttgaata
     2641 gctctgcgtc cagtatctcg tgcagctaaa ggctacctca gtaagcgagt aacgcggccg
     2701 cttattaatt aagatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     2761 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     2821 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     2881 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     2941 tttattgcca aatgtttgaa cgatcgggga aattcgagct ctccggatcc cggtcggcat
     3001 ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     3061 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     3121 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     3181 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     3241 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     3301 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     3361 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     3421 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     3481 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     3541 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     3601 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     3661 gcagcgatcg catccatggc ctccgcgacc ggctgcagtt atcatcatca tcatagacac
     3721 acgaaataaa gtaatcagat tatcagttaa agctatgtaa tatttacacc ataaccaatc
     3781 aattaaaaaa tagatcagtt taaagaaaga tcaaagctca aaaaaataaa aagagaaaag
     3841 ggtcctaacc aagaaaatga aggagaaaaa ctagaaattt acctgcagaa cagcgggcag
     3901 ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt
     3961 caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
     4021 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
     4081 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
     4141 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
     4201 ggtgagttca ggctttttca tatcggggtc gtcctctcca aatgaaatga acttccttat
     4261 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     4321 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     4381 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     4441 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
     4501 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     4561 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
     4621 tagacgagag tgtcgtgctc caccatgttg acttaattaa aaggccgtac ggttaaccat
     4681 catttagtga cacaattaac ttaaatttag aagagactaa tttcaaccca caagatggaa
     4741 atgcaaggac aatgggttct tgaagttatt tggaaacaaa gcaacacaac acaatagctt
     4801 attcattagt tctcatccat taatttccat tgaagtccga aaacagaaac caaaaataca
     4861 ggatgatgaa atctcataaa gcaaaaacaa atgggaatgt agtttaagta caacaaacaa
     4921 agtggggtgg ctacagatgc tttgcaaatt tttcaatgac ttacggtacc ttaaagctca
     4981 tcatgcttgt acagctcgtc catgccgtga acagctcctc gcccttgctc acgaattcgg
     5041 ccgaggataa tgataggaga agtgaaaaga tgagaaagag aaaaagatta gtcttcatgg
     5101 aagcttctgc agaagtaaca ccaaacaaca gggtgagcat cgacaaaaga aacagtacca
     5161 agcaaataaa tagcgtatga aggcagggct aaaaaaatcc acatatagct gctgcatatg
     5221 ccatcatcca agtatatcaa gatcaaaata attataaaac atacttgttt attataatag
     5281 ataggtactc aaggttagag catatgaata gatgctgcat atgccatcat gtatatgcat
     5341 cagtaaaacc cacatcaaca tgtataccta tcctagatcg atatttccat ccatcttaaa
     5401 ctcgtaacta tgaagatgta tgacacacac atacagttcc aaaattaata aatacaccag
     5461 gtagtttgaa acagtattct actccgatct agaacgaatg aacgaccgcc caaccacacc
     5521 acatcatcac aaccaagcga acaaaaagca tctctgtata tgcatcagta aaacccgcat
     5581 caacatgtat acctatccta gatcgatatt tccatccatc atcttcaatt cgtaactatg
     5641 aatatgtatg gcacacacat acagatccaa aattaataaa tccaccaggt agtttgaaac
     5701 agaattaatt ctactccgat ctagaacgac cgcccaacca gaccacatca tcacaaccaa
     5761 gacaaaaaaa agcatgaaaa gatgacccga caaacaagtg cacggcatat attgaaataa
     5821 aggaaaaggg caaaccaaac cctatgcaac gaaacaaaaa aaatcatgaa atcgatcccg
     5881 tctgcggaac ggctagagcc atcccaggat tccccaaaga gaaacactgg caagttagca
     5941 atcagaacgt gtctgacgta caggtcgcat ccgtgtacga acgctagcag cacggatcta
     6001 acacaaacac ggatctaaca caaacatgaa cagaagtaga actaccgggc cctaaccatg
     6061 gaccggaacg ccgatctaga gaaggtagag aggggggggg ggggaggacg agcggcgtac
     6121 cttgaagcgg aggtgccgac gggtggattt gggggagatc tggttgtgtg tgtgtgcgct
     6181 ccgaacaaca cgaggttggg gaaagagggt gtggaggggg tgtctattta ttacggcggg
     6241 cgaggaaggg aaagcgaagg agcggtggga aaggaatccc ccgtagctgc cggtgccgtg
     6301 agaggaggag gaggccgcct gccgtgccgg ctcacgtctg ccgctccgcc acgcaatttc
     6361 tggatgccga cagcggagca agtccaacgg tggagcggaa ctctcgagag gggtccagag
     6421 gcagcgacag agatgccgtg ccgtctgctt cgcttggccc gacgcgacgc tgctggttcg
     6481 ctggttggtg tccgttagac tcgtcgacgg cgtttaacag gctggcatta tctactcgaa
     6541 acaagaaaaa tgtttcctta gtttttttaa tttcttaaag ggtatttgtt taatttttag
     6601 tcactttatt ttattctatt ttatatctaa attattaaat aaaaaaacta aaatagagtt
     6661 ttagttttct taatttagag gctaaaatag aataaaatag atgtactaaa aaaattagtc
     6721 tataaaaacc attaacccta aaccctaaat ggatgtacta ataaaatgga tgaagtatta
     6781 tataggtgaa gctatttgca aaaaaaaagg agaacacatg cacactaaaa agataaaact
     6841 gtagagtcct gttgtcaaaa tactcaattg tcctttagac catgtctaac tgttcattta
     6901 tatgattctc taaaacactg atattattgt agtactatag attatattat tcgtagagta
     6961 aagtttaaat atatgtataa agatagataa actgcacttc aaacaagtgt gacaaaaaaa
     7021 atatgtggta attttttata acttagacat gcaatgctca ttatctctag agaggggcac
     7081 gaccgggtca cgctgcactg caggaattca agctggcatc gagattcaaa ctagtctcga
     7141 gtcgacaggc ctttctaacc tgtcgaggat acgtgataag cgggagctta gcggagctcg
     7201 ttgcaatcaa agctcgatac gtcgagactt agaggatcta cgacagtcca cagaacaggc
     7261 cttgacagga tatattggcg ggtaaactaa gtcgctgtat gtgtttgttt gtttaaaccc
     7321 gggaggctcg cggcggacgc acgacgccgg ggcgagacca taggcgatct cctaaatcaa
     7381 tagtagctgt aacctcgaag cgtttcactt gtaacaacga ttgagaattt ttgtcataaa
     7441 attgaaatac ttggttcgca tttttgtcat ccgcggtcag ccgcaattct gacgaactgc
     7501 ccatttagct ggagatgatt gtacatcctt cacgtgaaaa tttctcaagc gctgtgaaca
     7561 agggttcaga ttttagattg aaaggtgagc cgttgaaaca cgttcttctt gtcgatgacg
     7621 acgtcgctat gcggcatctt attattgaat accttacgat ccacgccttc aaagtgaccg
     7681 cggtagccga cagcacccag ttcacaagag tactctcttc cgcgacggtc gatgtcgtgg
     7741 ttgttgatct aaatttaggt cgtgaagatg ggctcgagat cgttcgtaat ctggcggcaa
     7801 agtctgatat tccaatcata attatcagtg gcgaccgcct tgaggagacg gataaagttg
     7861 ttgcactcga gctaggagca agtgatttta tcgctaagcc gttcagtatc agagagtttc
     7921 tagcacgcat tcgggttgcc ttgcgcgtgc gccccaacgt tgtccgctcc aaagaccgac
     7981 ggtctttttg ttttactgac tggacactta atctcaggca acgtcgcttg atgtccgaag
     8041 ctggcggtga ggtgaaactt acggcaggtg agttcaatct tctcctcgcg tttttagaga
     8101 aaccccgcga cgttctatcg cgcgagcaac ttctcattgc cagtcgagta cgcgacgagg
     8161 aggtttatga caggagtata gatgttctca ttttgaggct gcgccgcaaa cttgaggcgg
     8221 atccgtcaag ccctcaactg ataaaaacag caagaggtgc cggttatttc tttgacgcgg
     8281 acgtgcaggt ttcgcacggg gggacgatgg cagcctgagc caattgcatt tgcctcttaa
     8341 ttatctggct caaagggtga ctgaggagta agcgatgtgc ccatcacact gcccgggcat
     8401 gcaagctgat ctggatct
//
