pBlueBacmsGCB1His vector (V009147)

LOCUS       V009147                 6910 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009147
VERSION     V009147
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6910)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6910)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             195..2054
                     /gene="Gucy1b1"
                     /label="Guanylate cyclase soluble subunit beta-1"
                     /note="Guanylate cyclase soluble subunit beta-1 from Mus
                     musculus. Accession#: O54865"
     CDS             2079..2120
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             2130..2147
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    2172..2304
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_recomb     2317..3022
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     promoter        3595..3699
                     /label="AmpR promoter"
     CDS             3700..4557
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4731..5319
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5496..6608)
                     /codon_start=1
                     /note="unnamed protein product; 5' lacZ fragment"
                     /protein_id="SJL87782.1"
                     /translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT
                     VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRL
                     PSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
                     VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGY
                     ADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLL
                     NGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
     regulatory      complement(6609..6883)
                     /label="AcNPV-ETL promoter"
                     /note="AcNPV-ETL promoter"
                     /regulatory_class="promoter"