LOCUS       V009147                 6910 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009147
VERSION     V009147
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6910)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6910)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             195..2054
                     /gene="Gucy1b1"
                     /label="Guanylate cyclase soluble subunit beta-1"
                     /note="Guanylate cyclase soluble subunit beta-1 from Mus
                     musculus. Accession#: O54865"
     CDS             2079..2120
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             2130..2147
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    2172..2304
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_recomb     2317..3022
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     promoter        3595..3699
                     /label="AmpR promoter"
     CDS             3700..4557
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4731..5319
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5496..6608)
                     /codon_start=1
                     /note="unnamed protein product; 5' lacZ fragment"
                     /protein_id="SJL87782.1"
                     /translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT
                     VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRL
                     PSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
                     VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGY
                     ADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLL
                     NGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
     regulatory      complement(6609..6883)
                     /label="AcNPV-ETL promoter"
                     /note="AcNPV-ETL promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
      121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat
      181 tgcccttgga caccatgtac ggtttcgtga accatgccct ggagctgctg gtgatccgca
      241 attatggtcc cgaggtgtgg gaagacatca aaaaagaggc acagctggat gaagaaggcc
      301 agtttcttgt cagaataata tacgatgatt ccaaaacata tgacctggtg gctgctgcaa
      361 gcaaagtcct caacctcaat gctggcgaaa tcctgcagat gtttgggaag atgtttttcg
      421 tcttctgcca ggagtctggc tatgatacca tcttgcgtgt cctgggatct aatgtcagag
      481 agtttttgca gaacctcgat gccctgcatg accacctcgc caccatttac ccagggatgc
      541 gcgcgccttc cttcaggtgc accgatgcgg agaaaggcaa agggctcatc ctgcactact
      601 actcggaaag agaggggctt caggacatcg tgatcgggat tatcaagact gttgctcaac
      661 agatacacgg cactgagata gacatgaagg tgattcagca aagaaatgaa gaatgtgatc
      721 acacccagtt tttaattgaa gaaaaggaat caaaagaaga ggatttttat gaagatctgg
      781 ataggtttga agagaatggt acccaggaat cacgtatcag cccttacacc ttctgcaaag
      841 catttccttt tcacatcata tttgaccgga acctagtggt cactcagtgt ggcaatgcca
      901 tctacagagt gctcccccag ctccagcctg ggaactgcag ccttctgtct gtcttctctc
      961 tggtccgccc tcacatcgac atcagtttcc atgggattct ttcacacatc aatacagtct
     1021 ttgtactgag aagcaaggaa gggttgctgg atgtggagaa acttgagtgt gaggatgaac
     1081 tgactggagc agagattagc tgcttacgtc tcaaaggcca aatgatctac ttaccagaag
     1141 cagatagcat cctcttcctc tgttcaccaa gtgtgatgaa cctggacgac ctaacaagaa
     1201 gaggcctgta tctgagtgac atccctctcc acgatgctac ccgagacctg gttcttttgg
     1261 gagaacagtt ccgggaggag tacaaactga cacaagagct ggaaatcctc accgacaggc
     1321 tgcagctcac actgagagcc ttggaggatg agaagaaaaa gacagacaca ttgctgtatt
     1381 ctgtccttcc tccatctgtt gccaatgagc tgagacacaa gcgcccagtg cctgccaaaa
     1441 gatacgacaa tgtgaccatc ctcttcagcg gcattgtggg cttcaatgct ttctgtagca
     1501 agcatgcatc tggagaaggg gccatgaaga ttgtcaatct cctcaacgat ctctacaccc
     1561 gatttgacac actgactgat tcacgaaaaa acccatttgt ttacaaggtg gaaacagttg
     1621 gtgacaagta tatgacagtg agtggcttgc cagaaccttg tatccaccat gcacggtcca
     1681 tttgccacct ggctttagac atgatggaaa ttgctggtca agttcaagta gatggtgaat
     1741 ctgttcagat aacaatcggg atccataccg gggaggtggt gacaggtgtg attggacagc
     1801 ggatgcctcg gtattgtctc tttgggaata ccgtcaacct cacaagcagg acagaaacca
     1861 caggagaaaa gggaaagata aatgtttccg aatatacata caggtgtctc atgtctccag
     1921 aaaactcgga tccactgttc catttggagc acagaggccc agtgtctatg aagggcaaga
     1981 aggaaccaat gcaagtctgg ttcctatcca ggaaaaatac aggcacggag gaaacaaatg
     2041 aggaggatga aaacaagggc aattcgaagc ttaggcctgg taagcctatc cctaaccctc
     2101 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga cgtctctagc
     2161 ttggagtcga caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2221 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2281 tcaatgtatc ttatcatctc tcgactctgc tgaagaggag gaaattctcc ttgaagtttc
     2341 cctggtgttc aaagtaaagg agtttgcacc agacgcacct ctgttcactg gtccggcgta
     2401 ttaaaacacg atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg
     2461 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg
     2521 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa
     2581 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
     2641 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
     2701 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
     2761 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
     2821 actcgacgta aacacgttaa ataaagcctg gacatattta acatcgggcg tgttagcttt
     2881 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
     2941 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
     3001 tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct
     3061 aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa
     3121 catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat
     3181 cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc
     3241 agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt
     3301 actggtttcg ggcgatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     3361 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     3421 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     3481 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3541 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3601 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3661 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3721 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3781 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3841 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3901 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3961 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4021 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4081 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4141 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4201 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4261 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4321 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4381 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4441 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4501 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4561 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4621 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4681 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4741 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4801 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4861 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     4921 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4981 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5041 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5101 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5161 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5221 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5281 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5341 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5401 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5461 acgaccgagc gcagcgagtc agtgagcgag gaagcatcct gcaccatcgt ctgctcatcc
     5521 atgacctgac catgcagagg atgatgctcg tgacggttaa cgcctcgaat cagcaacggc
     5581 ttgccgttca gcagcagcag accattttca atccgcacct cgcggaaacc gacatcgcag
     5641 gcttctgctt caatcagcgt gccgtcggcg gtgtgcagtt caaccaccgc acgatagaga
     5701 ttcgggattt cggcgctcca cagtttcggg ttttcgacgt tcagacgtag tgtgacgcga
     5761 tcggcataac caccacgctc atcgataatt tcaccgccga aaggcgcggt gccgctggcg
     5821 acctgcgttt caccctgcca taaagaaact gttacccgta ggtagtcacg caactcgccg
     5881 cacatctgaa cttcagcctc cagtacagcg cggctgaaat catcattaaa gcgagtggca
     5941 acatggaaat cgctgatttg tgtagtcggt ttatgcagca acgagacgtc acggaaaatg
     6001 ccgctcatcc gccacatatc ctgatcttcc agataactgc cgtcactcca gcgcagcacc
     6061 atcaccgcga ggcggttttc tccggcgcgt aaaaatgcgc tcaggtcaaa ttcagacggc
     6121 aaacgactgt cctggccgta accgacccag cgcccgttgc accacagatg aaacgccgag
     6181 ttaacgccat caaaaataat tcgcgtctgg ccttcctgta gccagctttc atcaacatta
     6241 aatgtgagcg agtaacaacc cgtcggattc tccgtgggaa caaacggcgg attgaccgta
     6301 atgggatagg tcacgttggt gtagatgggc gcatcgtaac cgtgcatctg ccagtttgag
     6361 gggacgacga cagtatcggc ctcaggaaga tcgcactcca gccagctttc cggcaccgct
     6421 tctggtgccg gaaaccaggc aaagcgccat tcgccattca ggctgcgcaa ctgttgggaa
     6481 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     6541 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggga
     6601 tctatcattt ttagcagtga ttctaattgc agctgctctt tgatacaact aattttacga
     6661 cgacgatgcg agcttttatt caaccgagcg tgcatgtttg caatcgtgca agcgttatca
     6721 atttttcatt atcgtattgt tgcacatcaa caggctggac accacgttga actcgccgca
     6781 gttttgcggc aagttggacc cgccgcgcat ccaatgcaaa ctttccgaca ttctgttgcc
     6841 tacgaacgat tgattctttg tccattgatc gaagcgagtg ccttcgactt tttcgtgtcc
     6901 agtgtggctt
//
