pBlueBacmsGCA1s vector (V009149)

LOCUS       40924_6741        6592 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Insect expression vector pBlueBacmsGCA1s, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6592)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6592)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             214..1704
                     /codon_start=1
                     /note="unnamed protein product; mGucy1a3 splice variant"
                     /protein_id="SJL87783.1"
                     /translation="MFCRKFKDLKITGECPFSLLAPGIIKAAARILYESHVEVSLMPPC
                     FRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPASLFCKTFPFHFMLDRDLAI
                     LQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNSV
                     KKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIG
                     EQARAQDGLKKRLGKLKATLEHAHQALEEEKKRTVDLLCSIFPSEVAQQLWQGQIVQAK
                     KFSEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCV
                     AGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPR
                     YCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPG
                     ICHFLDAYHHQGPNSKPWFQDKDVEDGNANFLGKASGVD"
     CDS             1761..1802
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             1812..1829
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    1854..1986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     1999..2704
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        3277..3381
                     /label=AmpR promoter
     CDS             3382..4239
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4413..5001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5178..6290)
                     /codon_start=1
                     /note="unnamed protein product; lacZ fragment"
                     /protein_id="SJL87786.1"
                     /translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT
                     VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRL
                     PSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
                     VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGY
                     ADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLL
                     NGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
     regulatory      complement(6291..6565)
                     /label=AcNPV-ETL promoter
                     /note="AcNPV-ETL promoter"
                     /regulatory_class="promoter"