LOCUS       40924_6741        6592 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Insect expression vector pBlueBacmsGCA1s, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6592)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6592)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             214..1704
                     /codon_start=1
                     /note="unnamed protein product; mGucy1a3 splice variant"
                     /protein_id="SJL87783.1"
                     /translation="MFCRKFKDLKITGECPFSLLAPGIIKAAARILYESHVEVSLMPPC
                     FRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPASLFCKTFPFHFMLDRDLAI
                     LQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNSV
                     KKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIG
                     EQARAQDGLKKRLGKLKATLEHAHQALEEEKKRTVDLLCSIFPSEVAQQLWQGQIVQAK
                     KFSEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCV
                     AGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPR
                     YCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPG
                     ICHFLDAYHHQGPNSKPWFQDKDVEDGNANFLGKASGVD"
     CDS             1761..1802
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             1812..1829
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    1854..1986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     1999..2704
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        3277..3381
                     /label=AmpR promoter
     CDS             3382..4239
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4413..5001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5178..6290)
                     /codon_start=1
                     /note="unnamed protein product; lacZ fragment"
                     /protein_id="SJL87786.1"
                     /translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT
                     VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRL
                     PSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
                     VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGY
                     ADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLL
                     NGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
     regulatory      complement(6291..6565)
                     /label=AcNPV-ETL promoter
                     /note="AcNPV-ETL promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
      121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat
      181 tgcccttagc cactgccaag ctccaggaac accatgttct gcaggaagtt caaggatctc
      241 aagatcacag gggagtgtcc tttctcctta ctggcccctg gtatcattaa agcggctgct
      301 cgcatactgt acgaaagcca cgtggaggtg tccctgatgc ctccctgctt ccgaagtgac
      361 tgtaccgagt ttgtgaacca gccctatttg ctctactccg ttcatgtgaa gagcaccaag
      421 ccgtccctgt ccccaggcaa gccccagtcc tcgctagtga tccccgcttc gctcttctgc
      481 aagactttcc cgttccattt catgctggac cgagacctgg ccatcctgca gctgggtaac
      541 ggcatcagaa ggctggtgaa caagagggac ttccaaggga agcccaactt tgaagagttc
      601 tttgaaattc taactcccaa aatcaaccag acatttagtg gcatcatgac aatgttgaac
      661 atgcagtttg tcatccgggt gaggagatgg gataactcgg tgaagaaatc gtcaagggtt
      721 atggatctca aaggtcaaat gatctacatc gttgaatcca gtgccatctt gttcttaggg
      781 tcaccatgtg tggacaggct ggaagatttc acaggacggg ggctctatct gtccgacatc
      841 ccaattcata acgccctgag ggatgttgtc ttgatagggg agcaggcacg ggctcaagat
      901 ggcctcaaga agaggttggg gaagctgaag gcaaccctgg agcatgccca ccaagccctg
      961 gaggaagaga agaagaggac agtggatctg ctgtgctcta tcttcccctc tgaggttgct
     1021 cagcagctgt ggcaaggaca aattgtgcaa gccaagaaat tcagcgaggt caccatgctt
     1081 ttctcagata tcgtagggtt cactgctatc tgctctcagt gttcacctct gcaggtcatc
     1141 acgatgctca acgctctcta cactcgcttt gaccagcagt gtggagagct ggatgtctac
     1201 aaggtggaga ccatcgggga tgcatattgt gtggcaggtg gattgcacag agagagtgac
     1261 acccatgctg tccagatagc actgatggcc ctgaagatga tggagctctc caatgaggtc
     1321 atgtctcccc acggagaacc tatcaagatg cgaattggac tacattctgg atcagtgttt
     1381 gctggagttg tcggagtgaa gatgccccgg tattgcctgt ttggaaacaa tgtcactctg
     1441 gctaacaaat ttgaatcctg cagtgtgcct cggaaaatca atgtcagccc caccacatac
     1501 aggttactca aagactgtcc tggctttgtg ttcaccccga gatcaaggga ggagcttcca
     1561 ccaaacttcc ctagtgacat tcctgggatc tgtcactttc tggatgctta tcaccatcaa
     1621 ggacctaatt ccaaaccatg gttccaggat aaagatgtgg aagatggaaa cgccaacttc
     1681 ttaggcaaag cgtcaggggt agattagtga gccacatgct cttatgtttg atgcctaagg
     1741 gcaattcgaa gcttaggcct ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     1801 cgcgtaccgg tcatcatcac catcaccatt gacgtctcta gcttggagtc gacaacttgt
     1861 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1921 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatc
     1981 tctcgactct gctgaagagg aggaaattct ccttgaagtt tccctggtgt tcaaagtaaa
     2041 ggagtttgca ccagacgcac ctctgttcac tggtccggcg tattaaaaca cgatacattg
     2101 ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg
     2161 tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat
     2221 caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta
     2281 aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc
     2341 taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt
     2401 gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg
     2461 cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt
     2521 aaataaagcc tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc
     2581 gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg
     2641 acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg
     2701 aattatttct gattgcgggc gtttttgggc gggtttcaat ctaactgtgc ccgattttaa
     2761 ttcagacaac acgttagaaa gcgatggtgc aggcggtggt aacatttcag acggcaaatc
     2821 tactaatggc ggcggtggtg gagctgatga taaatctacc atcggtggag gcgcaggcgg
     2881 ggctggcggc ggaggcggag gcggaggtgg tggcggtgat gcagacggcg gtttaggctc
     2941 aaatgtctct ttaggcaaca cagtcggcac ctcaactatt gtactggttt cgggcgatgg
     3001 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     3061 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3121 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     3181 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     3241 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3301 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3361 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3421 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3481 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3541 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3601 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     3661 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3721 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3781 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3841 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3901 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3961 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4021 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4081 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4141 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4201 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4261 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4321 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4381 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4441 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4501 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4561 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4621 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4681 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4741 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4801 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4861 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4921 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4981 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5041 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5101 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5161 tcagtgagcg aggaagcatc ctgcaccatc gtctgctcat ccatgacctg accatgcaga
     5221 ggatgatgct cgtgacggtt aacgcctcga atcagcaacg gcttgccgtt cagcagcagc
     5281 agaccatttt caatccgcac ctcgcggaaa ccgacatcgc aggcttctgc ttcaatcagc
     5341 gtgccgtcgg cggtgtgcag ttcaaccacc gcacgataga gattcgggat ttcggcgctc
     5401 cacagtttcg ggttttcgac gttcagacgt agtgtgacgc gatcggcata accaccacgc
     5461 tcatcgataa tttcaccgcc gaaaggcgcg gtgccgctgg cgacctgcgt ttcaccctgc
     5521 cataaagaaa ctgttacccg taggtagtca cgcaactcgc cgcacatctg aacttcagcc
     5581 tccagtacag cgcggctgaa atcatcatta aagcgagtgg caacatggaa atcgctgatt
     5641 tgtgtagtcg gtttatgcag caacgagacg tcacggaaaa tgccgctcat ccgccacata
     5701 tcctgatctt ccagataact gccgtcactc cagcgcagca ccatcaccgc gaggcggttt
     5761 tctccggcgc gtaaaaatgc gctcaggtca aattcagacg gcaaacgact gtcctggccg
     5821 taaccgaccc agcgcccgtt gcaccacaga tgaaacgccg agttaacgcc atcaaaaata
     5881 attcgcgtct ggccttcctg tagccagctt tcatcaacat taaatgtgag cgagtaacaa
     5941 cccgtcggat tctccgtggg aacaaacggc ggattgaccg taatgggata ggtcacgttg
     6001 gtgtagatgg gcgcatcgta accgtgcatc tgccagtttg aggggacgac gacagtatcg
     6061 gcctcaggaa gatcgcactc cagccagctt tccggcaccg cttctggtgc cggaaaccag
     6121 gcaaagcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     6181 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     6241 acgccagggt tttcccagtc acgacgttgt aaaacgacgg gatctatcat ttttagcagt
     6301 gattctaatt gcagctgctc tttgatacaa ctaattttac gacgacgatg cgagctttta
     6361 ttcaaccgag cgtgcatgtt tgcaatcgtg caagcgttat caatttttca ttatcgtatt
     6421 gttgcacatc aacaggctgg acaccacgtt gaactcgccg cagttttgcg gcaagttgga
     6481 cccgccgcgc atccaatgca aactttccga cattctgttg cctacgaacg attgattctt
     6541 tgtccattga tcgaagcgag tgccttcgac tttttcgtgt ccagtgtggc tt
//
