pBLCAT3T-p5 vector (V009163)

LOCUS       ampR_gene;_beta-        5653 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBLCAT3T-p5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    ampR gene; beta-lactamase; cat gene; chloramphenicol acetyltra.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Luckow B, Schutz G.
  TITLE     CAT constructions with multiple unique restriction sites for the 
            functional analysis of eukaryotic promoters and regulatory elements
  JOURNAL   Nucleic Acids Res. 15 (13), 5490 (1987)
  PUBMED    3037497
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Lobo SM, Hernandez N.
  TITLE     A 7 bp mutation converts a human RNA polymerase II snRNA promoter 
            into an RNA polymerase III promoter
  JOURNAL   Cell 58 (1), 55-67 (1989)
  PUBMED    2752422
REFERENCE   3  (bases 1 to 5653)
  AUTHORS   Principaud E, Spohr G.
  TITLE     Xenopus laevis c-myc I and II genes: molecular structure and 
            developmental expression
  JOURNAL   Nucleic Acids Res. 19 (11), 3081-3088 (1991)
  PUBMED    2057364
REFERENCE   4  (bases 1 to 5653)
  AUTHORS   Bernhardt K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2007) Bernhardt K., The Gurdon Institute, 
            University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, 
            UNITED KINGDOM
REFERENCE   5  (bases 1 to 5653)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 5653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1987"; volume: "15"; issue: "13"; pages: "5490"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Cell"; 
            date: "1989"; volume: "58"; issue: "1"; pages: "55-67"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1991"; volume: "19"; issue: "11"; pages: 
            "3081-3088"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (16-APR-2007) Bernhardt K., The Gurdon Institute, University of 
            Cambridge, Tennis Court Road, Cambridge CB2 1QN, UNITED KINGDOM"
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          755..1753
                     /chromosome="6"
                     /map="6F2"
                     /mol_type="other DNA"
                     /db_xref="taxon:10090"
                     /organism="Mus musculus"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      440..453
                     /note="this is a nonfunctional RNA polymerase II terminator
                     (3' box), termination depends on the presence of an 
                     appropriate snRNA promoter upstream such as in the human U1
                     and U2 snRNA genes"
                     /citation=[2]
                     /citation=[3]
                     /regulatory_class="terminator"
     regulatory      474..481
                     /note="this is a functional RNA polymerase III terminator
                     from the human U6 snRNA gene"
                     /citation=[2]
                     /citation=[3]
                     /regulatory_class="terminator"
     regulatory      complement(755..1753)
                     /gene="Nanog"
                     /note="reversed 999 bp of the mouse Nanog promoter -1000 to
                     -2 with respect to the transcriptional start site (-1190 to
                     -192 with respect to the Nanog start codon)"
                     /regulatory_class="promoter"
     gene            complement(755..1753)
                     /gene="Nanog"
                     /label=Nanog
     CDS             1797..2453
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     intron          2622..2687
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2817..2837
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    3262..3396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3432..3448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3456..3472)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3480..3510)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3525..3546)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3834..4422)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4596..5453)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5454..5558)
                     /label=AmpR promoter