LOCUS       ampR_gene;_beta-        5653 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBLCAT3T-p5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    ampR gene; beta-lactamase; cat gene; chloramphenicol acetyltra.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Luckow B, Schutz G.
  TITLE     CAT constructions with multiple unique restriction sites for the 
            functional analysis of eukaryotic promoters and regulatory elements
  JOURNAL   Nucleic Acids Res. 15 (13), 5490 (1987)
  PUBMED    3037497
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Lobo SM, Hernandez N.
  TITLE     A 7 bp mutation converts a human RNA polymerase II snRNA promoter 
            into an RNA polymerase III promoter
  JOURNAL   Cell 58 (1), 55-67 (1989)
  PUBMED    2752422
REFERENCE   3  (bases 1 to 5653)
  AUTHORS   Principaud E, Spohr G.
  TITLE     Xenopus laevis c-myc I and II genes: molecular structure and 
            developmental expression
  JOURNAL   Nucleic Acids Res. 19 (11), 3081-3088 (1991)
  PUBMED    2057364
REFERENCE   4  (bases 1 to 5653)
  AUTHORS   Bernhardt K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2007) Bernhardt K., The Gurdon Institute, 
            University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, 
            UNITED KINGDOM
REFERENCE   5  (bases 1 to 5653)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 5653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1987"; volume: "15"; issue: "13"; pages: "5490"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Cell"; 
            date: "1989"; volume: "58"; issue: "1"; pages: "55-67"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1991"; volume: "19"; issue: "11"; pages: 
            "3081-3088"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (16-APR-2007) Bernhardt K., The Gurdon Institute, University of 
            Cambridge, Tennis Court Road, Cambridge CB2 1QN, UNITED KINGDOM"
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          755..1753
                     /chromosome="6"
                     /map="6F2"
                     /mol_type="other DNA"
                     /db_xref="taxon:10090"
                     /organism="Mus musculus"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      440..453
                     /note="this is a nonfunctional RNA polymerase II terminator
                     (3' box), termination depends on the presence of an 
                     appropriate snRNA promoter upstream such as in the human U1
                     and U2 snRNA genes"
                     /citation=[2]
                     /citation=[3]
                     /regulatory_class="terminator"
     regulatory      474..481
                     /note="this is a functional RNA polymerase III terminator
                     from the human U6 snRNA gene"
                     /citation=[2]
                     /citation=[3]
                     /regulatory_class="terminator"
     regulatory      complement(755..1753)
                     /gene="Nanog"
                     /note="reversed 999 bp of the mouse Nanog promoter -1000 to
                     -2 with respect to the transcriptional start site (-1190 to
                     -192 with respect to the Nanog start codon)"
                     /regulatory_class="promoter"
     gene            complement(755..1753)
                     /gene="Nanog"
                     /label=Nanog
     CDS             1797..2453
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     intron          2622..2687
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2817..2837
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    3262..3396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3432..3448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3456..3472)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3480..3510)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3525..3546)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3834..4422)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4596..5453)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5454..5558)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagctcg
      421 acccccctga ctttctggag tttcaaaaac agaccgtacg ccaagggtca tgtctttttt
      481 cgtattggtt tgtgtcttag ttgttaatcc tacagtggag ggatcgatcc tctagcttcc
      541 tggagcgcca ctcgccctac ttccgcagcc acagtgcgca gattaggagc gccacttctt
      601 tttgtcactt gaaaaacatg taaaaataat gtactaggag acactttcaa taaaggcaaa
      661 tgtttttatt tgtacactct cgggtgatta tttacccccc acccttgccg tctgcgccgt
      721 ttctagcgac ctgcaggtcg actctagagg atccaggcca acggctcaag gcgatagatt
      781 taaagggtag gtcctgctcc tcccccctca agcctcctac cctacccacc ccctattctc
      841 ccaggcaccc aggcggcacc cacgccccac cagccctgtg aattcacagt taatcccacc
      901 tgcagggtcc accatggaca ttgtaatgca aaagaagctg taaggtgacc cagactggga
      961 gggagggaaa gcttaggggg catcctctga tctaaagaca tccccccccc ccaaaaagag
     1021 gctttacctc atttcacttt attcatcttt taaccacggc tgcacctcca gaccctggcg
     1081 atctctagtg ggaagtttca ggtcaagtgg gggatctgag tttcctcttc cattctttct
     1141 acagacatga aagagtcaga ccttgctgcc aaagtctctg tttatacacg gctttccttt
     1201 cagcactcag cgtttcttgc ttgctcttca cattgggaaa cctggggaaa tcttccctcc
     1261 aaaagtgcgg ctttttaacg caagtctgaa gaaagagcct gtgtcctgct taagactcgg
     1321 gctaccttag taagttggtc catgctttgg ttttgagaca ggtttttctc tgttgccttg
     1381 agtagcttat actcagtatg tagaccaggc ttgtctacca ccatgcccaa tttaaggagt
     1441 gtttaaatta atgtagaggc cttctgtttt gttttgagac aagttctcac tgtaaaacct
     1501 aggctggctt cagacttact gcaatcccaa gtgcctcagc cgtctaagca atggaagaag
     1561 aaatttatcg tatttcaaat ttggttttgg tcatttcttt gagatagagt ttcacgtacc
     1621 cgagactggc ctcacagtct attgctctag ctggtcccaa actcctgatc ttcctatttc
     1681 cacatcccaa gttcaaagtt tgccgatcag tccttgtgct cacagataag tgagtgaaag
     1741 cactctaacc cagctcgagg agcttggcga gattttcagg agctaaggaa gctaaaatgg
     1801 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     1861 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     1921 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     1981 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     2041 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     2101 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     2161 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     2221 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     2281 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     2341 aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg
     2401 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt
     2461 tttaaggcag ttattggtgc ccttaaacgc ctggtgctac gcctgaataa gtgataataa
     2521 gcggatgaat ggcagaaatt cgccggatct ttgtgaagga accttacttc tgtggtgtga
     2581 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     2641 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     2701 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     2761 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     2821 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     2881 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     2941 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     3001 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     3061 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     3121 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     3181 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3241 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3361 tccaaactca tcaatgtatc ttatcatgtc tggatcgatc cccgggtacc gagctcgaat
     3421 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3481 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3541 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     3601 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     3661 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     3721 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     3781 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3841 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3901 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3961 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4021 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4081 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4141 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4201 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4261 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4321 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4381 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4441 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4501 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4561 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4621 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4681 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     4741 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4801 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     4861 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     4921 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4981 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5041 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5101 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5161 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5221 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5281 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5341 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5401 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5461 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5521 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5581 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     5641 aggccctttc gtc
//
