EGFP-Tubulin-6 vector (V010501)

MSDS

Basic Vector Information

Vector Name:: EGFP-Tubulin-6

Antibiotic Resistance:: Kanamycin

Length:: 6045 bp

Type:: Mammalian Expression

Replication origin:: ori

Selection Marker:: Neomycin (select with G418)

Copy Number:: High Copy

Promoter:: CMV

5' Primer:: CMV-F

3' Primer:: SV40pA-R

EGFP-Tubulin-6 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

EGFP-Tubulin-6 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       EGFP-Tubulin-6.        6045 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Localization: Microtubules, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    EGFP-Tubulin-6.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 6045)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6045)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; pages: " 10.1101/pdb.top63"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..353
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        354..557
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             602..1318
                     /label=EGFP
                     /note="enhanced GFP"
     CDS             1337..2689
                     /gene="Tuba1b"
                     /label=Tuba1b
                     /note="Tubulin alpha-1B chain from Rattus norvegicus. 
                     Accession#: Q6P9V9"
     polyA_signal    2822..2943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2950..3405)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3432..3536
                     /label=AmpR promoter
     promoter        3538..3895
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3930..4721
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(4912..4931)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4956..5003
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5332..5920
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        6035..6045
                     /label=CMV
                     /note="Human cytomegalovirus immediate early
                     enhancer/promoter"

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