LOCUS EGFP-Tubulin-6. 6045 bp DNA circular SYN 13-MAY-2021 DEFINITION Localization: Microtubules, Excitation: 488, Emission: 507. ACCESSION . VERSION . KEYWORDS EGFP-Tubulin-6. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6045) AUTHORS Rizzo MA, Davidson MW, Piston DW TITLE Fluorescent protein tracking and detection: fluorescent protein structure and color variants. JOURNAL Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 10.1101/pdb.top63. PUBMED 20150100 REFERENCE 2 (bases 1 to 6045) TITLE Direct Submission REFERENCE 3 (bases 1 to 6045) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring Harb Protoc."; date: "2009-12"; pages: " 10.1101/pdb.top63" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..6045 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 50..353 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 354..557 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 602..1318 /label=EGFP /note="enhanced GFP" CDS 1337..2689 /gene="Tuba1b" /label=Tuba1b /note="Tubulin alpha-1B chain from Rattus norvegicus. Accession#: Q6P9V9" polyA_signal 2822..2943 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(2950..3405) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3432..3536 /label=AmpR promoter promoter 3538..3895 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 3930..4721 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" primer_bind complement(4912..4931) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4956..5003 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5332..5920 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 6035..6045 /label=CMV /note="Human cytomegalovirus immediate early enhancer/promoter" ORIGIN 1 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 61 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 121 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 181 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 241 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 301 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 361 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 421 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 481 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg 541 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac 601 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 661 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 721 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 781 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 841 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 901 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 961 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 1021 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 1081 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 1141 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 1201 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 1261 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc 1321 cggactcaga tctcgagtgc gtgagtgcat ctccatccac gttggccagg ctggtgtcca 1381 gattggcaat gcctgctggg agctctactg cctggaacac ggcatccagc ccgatggcca 1441 gatgccaagt gacaagacca ttgggggagg agatgactcc ttcaacacct tcttcagtga 1501 gacgggcgct ggcaagcacg tgccccgggc tgtgtttgta gacttggaac ccacagtcat 1561 tgatgaagtt cgcactggca cctaccgcca gctcttccac cctgagcagc tcatcacagg 1621 caaggaagat gctgccaata actatgcccg agggcactac accattggca aggagatcat 1681 tgaccttgtg ttggaccgaa ttcgcaagct ggctgaccag tgcaccggtc ttcagggctt 1741 cttggttttc cacagctttg gtgggggaac tggttctggg ttcacctccc tgctcatgga 1801 acgtctctca gttgattatg gcaagaagtc caagctggag ttctccattt acccagcacc 1861 ccaggtttcc acagctgtag ttgagcccta caactccatc ctcaccaccc acaccaccct 1921 ggagcactct gattgtgcct tcatggtaga caatgaggcc atctatgaca tctgtcgtag 1981 aaacctcgat atcgagcgcc caacctacac taaccttaac cgccttatta gccagattgt 2041 gtcctccatc actgcttccc tgagatttga tggagccctg aatgttgacc tgacagaatt 2101 ccagaccaac ctggtgccct acccccgcat ccacttccct ctggccacat atgcccctgt 2161 catctctgct gagaaagcct accatgaaca gctttctgta gcagagatca ccaatgcttg 2221 ctttgagcca gccaaccaga tggtgaaatg tgaccctcgc catggtaaat acatggcttg 2281 ctgcctgttg taccgtggtg acgtggttcc caaagatgtc aatgctgcca ttgccaccat 2341 caaaaccaag cgcagcatcc agtttgtgga ttggtgcccc actggcttca aggttggcat 2401 caactaccag cctcccactg tggtgcctgg tggagacctg gccaaggtac agagagctgt 2461 gtgcatgctg agcaacacca cagccattgc tgaggcctgg gctcgcctgg accacaagtt 2521 tgacctgatg tatgccaagc gtgcctttgt tcactggtac gtgggtgagg ggatggagga 2581 aggcgagttt tcagaggccc gtgaagatat ggctgccctt gagaaggatt atgaggaggt 2641 tggtgtggat tctgttgaag gagagggtga ggaagaagga gaggaatact aaggatccac 2701 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt 2761 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 2821 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 2881 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 2941 ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa 3001 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat 3061 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg 3121 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac 3181 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta 3241 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag 3301 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg 3361 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc 3421 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 3481 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg 3541 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 3601 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 3661 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 3721 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 3781 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 3841 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg 3901 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 3961 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 4021 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 4081 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg 4141 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 4201 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 4261 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 4321 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 4381 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 4441 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 4501 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 4561 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 4621 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 4681 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 4741 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 4801 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 4861 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg 4921 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac 4981 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt 5041 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc 5101 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc 5161 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt 5221 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 5281 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 5341 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 5401 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 5461 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 5521 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 5581 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 5641 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 5701 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 5761 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 5821 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 5881 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 5941 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 6001 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaat //