pACYC184-ara-xylE vector (V009588)

LOCUS       V009588                 6369 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009588
VERSION     V009588
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Tsai Y, Liu M-C., Li Z-S., Liaw S-J.
  TITLE     The RNA chaperone Hfq is involved in stress tolerance and virulence
            in uropathogenic Proteus mirabilis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Tsai Y, Liu M-C., Li Z-S., Liaw S-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2014) Department of Clinical Laboratory Sciences
            and Medical Biotechnology, College of Medicine, National Taiwan
            University, 1 Chang-Te St., Taipei 100, Taiwan
REFERENCE   3  (bases 1 to 6369)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6369)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-JAN-2014) Department of Clinical Laboratory Sciences and Medical
            Biotechnology, College of Medicine, National Taiwan University, 1
            Chang-Te St., Taipei 100, Taiwan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(220..322)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        1505..1533
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             complement(1733..2653)
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"
     promoter        complement(2904..3188)
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             3215..4090
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     CDS             complement(join(5932..6369,1..219))
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"