LOCUS       V009588                 6369 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009588
VERSION     V009588
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Tsai Y, Liu M-C., Li Z-S., Liaw S-J.
  TITLE     The RNA chaperone Hfq is involved in stress tolerance and virulence
            in uropathogenic Proteus mirabilis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Tsai Y, Liu M-C., Li Z-S., Liaw S-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2014) Department of Clinical Laboratory Sciences
            and Medical Biotechnology, College of Medicine, National Taiwan
            University, 1 Chang-Te St., Taipei 100, Taiwan
REFERENCE   3  (bases 1 to 6369)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6369)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-JAN-2014) Department of Clinical Laboratory Sciences and Medical
            Biotechnology, College of Medicine, National Taiwan University, 1
            Chang-Te St., Taipei 100, Taiwan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(220..322)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        1505..1533
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             complement(1733..2653)
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"
     promoter        complement(2904..3188)
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             3215..4090
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     CDS             complement(join(5932..6369,1..219))
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     1681 atgggcgaat tccaagcttg aattcgcggc cgcaagtcgt accggaccat caggtcagca
     1741 cggtcatgaa tcgttcgttg agaatgcggt cgtggtaaaa gatcgccttg cccagctggt
     1801 cggtggtcca ggtcaccggt ttgtggtccg ggtagttgta atctcccccg cagaacactt
     1861 cgttgcggtt accggacggg tcgaagaagt agatggtctt gccgtgagtg aggccgtggc
     1921 gggttgggcc gatatcgata gatgtgtcgg tcatggagat caggtcggcg gcgcgaagca
     1981 agtcttccca ggtttcgagg tggaaggaca catgatggag gcggcctttt tccggatggt
     2041 gaatgaaggc cacgtcgtgg gccttggtcg acagactgag aaactgggcg acgcgcgtgc
     2101 cattttcgtc cagcacctgt tcggccagat agaaaccgag caccttggtg aacaggtcat
     2161 aggtcgccgg caattcgtcg ccatacatga gggcgtggtc gaaacgcaca gccgccatac
     2221 ctttcagatc gcgcggccat gcctcgggat tgacgtcatt caaaccccac tttccagtat
     2281 attccttgtc tgcatacaac tcgaagtgat gcccggaggg ggcctggaag cgcacgcgcc
     2341 ggccacaact gttcagttca cctgcgggta gctgctcaac ggcacagcca tatgccatca
     2401 gatcccgctc cagttgccgg agagcatcct catccacaac cttgaaaccc ataaaatcca
     2461 tgcccggctc gtcagcctcg cgtagcacca gggaaaactt atccacttcg gtccaagcct
     2521 tcagatagac acggccctgg tcgtcacggt ccatctcgat caggcccagc aactcgacgt
     2581 agtgttccag ggccttgctc atgtccagta cacgcagctg cacatggccc ggtcgcatta
     2641 cacctttgtt catgacgtca cctcttcata gttcatgttg tcaggtttgt ttccgccaac
     2701 gtggcgaaag tactcgatgg tcaagtcggt ttccggaaac acttcacagg ccagggccag
     2761 gaaaaaaaag ctcctcgaga aggtcgccgg tctgcttgtc cagttcgccg atcacggtca
     2821 tcggcgggaa tcgtagtcct tgatcgcggc cgcagatctg gatcccctct agagtcgacc
     2881 tgcagtagcc caaaaaaacg ggtatggaga aacagtagag agttgcgata aaaagcgtca
     2941 ggtaggatcc gctaatctta tggataaaaa tgctatggca tagcaaagtg tgacgccgtg
     3001 caaataatca atgtggactt ttctgccgtg attatagaca cttttgttac gcgtttttgt
     3061 catggctttg gtcccgcttt gttacagaat gcttttaata agcggggtta ccggtttggt
     3121 tagcgagaag agccagtaaa agacgcagtg acggcaatgt ctgatgcaat atggacaatt
     3181 ggtttcttct ctgaatggcg ggagtatgaa aagtatggct gaagcgcaaa atgatcccct
     3241 gctgccggga tactcgttta atgcccatct ggtggcgggt ttaacgccga ttgaggccaa
     3301 cggttatctc gattttttta tcgaccgacc gctgggaatg aaaggttata ttctcaatct
     3361 caccattcgc ggtcaggggg tggtgaaaaa tcagggacga gaatttgttt gccgaccggg
     3421 tgatattttg ctgttcccgc caggagagat tcatcactac ggtcgtcatc cggaggctcg
     3481 cgaatggtat caccagtggg tttactttcg tccgcgcgcc tactggcatg aatggcttaa
     3541 ctggccgtca atatttgcca atacggggtt ctttcgcccg gatgaagcgc accagccgca
     3601 tttcagcgac ctgtttgggc aaatcattaa cgccgggcaa ggggaagggc gctattcgga
     3661 gctgctggcg ataaatctgc ttgagcaatt gttactgcgg cgcatggaag cgattaacga
     3721 gtcgctccat ccaccgatgg ataatcgggt acgcgaggct tgtcagtaca tcagcgatca
     3781 cctggcagac agcaattttg atatcgccag cgtcgcacag catgtttgct tgtcgccgtc
     3841 gcgtctgtca catcttttcc gccagcagtt agggattagc gtcttaagct ggcgcgagga
     3901 ccaacgtatc agccaggcga agctgctttt gagcaccacc cggatgccta tcgccaccgt
     3961 cggtcgcaat gttggttttg acgatcaact ctatttctcg cgggtattta aaaaatgcac
     4021 cggggccagc ccgagcgagt tccgtgccgg ttgtgaagaa aaagtgaatg atgtagccgt
     4081 caagttgtca taattggtaa cgaatcagac aattgacggc ttgacggagt agcatagggt
     4141 ttgcagaatc cctgcttcgt ccatttgaca ggcacattat gcatgcacca ttccttgcgg
     4201 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     4261 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     4321 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     4381 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     4441 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     4501 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     4561 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     4621 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     4681 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     4741 taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     4801 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     4861 gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
     4921 cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca
     4981 aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg
     5041 catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag
     5101 gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga
     5161 gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt
     5221 cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcccctacgt gctgctgaag
     5281 ttgcccgcaa cagagagtgg aaccaaccgg tgataccacg atactatgac tgagagtcaa
     5341 cgccatgagc ggcctcattt cttattctga gttacaacag tccgcaccgc tgtccggtag
     5401 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     5461 catgcaactc gtaggacagg tgccggcagc gcccaacagt cccccggcca cggggcctgc
     5521 caccataccc acgccgaaac aagcgccctg caccattatg ttccggatct gcatcgcagg
     5581 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct aaccgttttt
     5641 atcaggctct gggaggcaga ataaatgatc atatcgtcaa ttattacctc cacggggaga
     5701 gcctgagcaa actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc
     5761 aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg
     5821 acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc
     5881 aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc
     5941 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     6001 acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     6061 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     6121 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     6181 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     6241 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     6301 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     6361 tgccatacg
//
