Basic Vector Information
FU-tet-o-hOct4 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
FU-tet-o-hOct4 vector Sequence
LOCUS FU-tet-o-hOct4. 9471 bp DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS FU-tet-o-hOct4. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9471) AUTHORS Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K TITLE A high-efficiency system for the generation and study of human induced pluripotent stem cells. JOURNAL Cell Stem Cell. 2008 Sep 11. 3(3):340-5. PUBMED 18786420 REFERENCE 2 (bases 1 to 9471) TITLE Direct Submission REFERENCE 3 (bases 1 to 9471) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem Cell. 2008 Sep 11. 3(3):340-5." COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..9471 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 618..820 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 2291..2332 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2610..2880 /label=tetracycline response element /note="contains seven copies of the tetracycline operator tetO" promoter 2913..2950 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2947..2971 /label=LNCX /note="Human CMV promoter, forward primer" CDS 3056..4135 /label=hOct4 /note="Homo sapiens Oct-4 gene. Encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression in adult tissues is associated with tumorigenesis." misc_feature 4163..4751 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4754..4770) /label=KS primer /note="common sequencing primer, one of multiple similar variants" LTR 5276..5456 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" polyA_signal 5488..5712 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5758..6186 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6200..6529 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 6577..6624 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 6643..7014 /label=BleoR /note="antibiotic-binding protein" polyA_signal 7147..7280 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7317..7333) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(7341..7357) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7365..7395) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(7410..7431) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7548..7565) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7719..8307) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8481..9338) /label=AmpR /note="beta-lactamase" promoter complement(9339..9443) /label=AmpR promoter
This page is informational only.