LOCUS FU-tet-o-hOct4. 9471 bp DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS FU-tet-o-hOct4. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9471) AUTHORS Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K TITLE A high-efficiency system for the generation and study of human induced pluripotent stem cells. JOURNAL Cell Stem Cell. 2008 Sep 11. 3(3):340-5. PUBMED 18786420 REFERENCE 2 (bases 1 to 9471) TITLE Direct Submission REFERENCE 3 (bases 1 to 9471) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem Cell. 2008 Sep 11. 3(3):340-5." COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..9471 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 618..820 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 2291..2332 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2610..2880 /label=tetracycline response element /note="contains seven copies of the tetracycline operator tetO" promoter 2913..2950 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2947..2971 /label=LNCX /note="Human CMV promoter, forward primer" CDS 3056..4135 /label=hOct4 /note="Homo sapiens Oct-4 gene. Encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression in adult tissues is associated with tumorigenesis." misc_feature 4163..4751 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4754..4770) /label=KS primer /note="common sequencing primer, one of multiple similar variants" LTR 5276..5456 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" polyA_signal 5488..5712 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5758..6186 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6200..6529 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 6577..6624 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 6643..7014 /label=BleoR /note="antibiotic-binding protein" polyA_signal 7147..7280 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7317..7333) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(7341..7357) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7365..7395) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(7410..7431) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7548..7565) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7719..8307) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8481..9338) /label=AmpR /note="beta-lactamase" promoter complement(9339..9443) /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc 2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat 2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg 2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact 2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga 2881 aaagtgaaag tcgagctcgg tacccgggtc gagtaggcgt gtacggtggg aggcctatat 2941 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga 3001 cctccataga agacaccggg accgatccag cctccgcggc cccgaattcg ccaccatggc 3061 gggacacctg gcttcggatt tcgccttctc gccccctcca ggtggtggag gtgatgggcc 3121 aggggggccg gagccgggct gggttgatcc tcggacctgg ctaagcttcc aaggccctcc 3181 tggagggcca ggaatcgggc cgggggttgg gccaggctct gaggtgtggg ggattccccc 3241 atgccccccg ccgtatgagt tctgtggggg gatggcgtac tgtgggcccc aggttggagt 3301 ggggctagtg ccccaaggcg gcttggagac ctctcagcct gagggcgaag caggagtcgg 3361 ggtggagagc aactccgatg gggcctcccc ggagccctgc accgtcaccc ctggtgccgt 3421 gaagctggag aaggagaagc tggagcaaaa cccggaggag tcccaggaca tcaaagctct 3481 gcagaaagaa ctcgagcaat ttgccaagct cctgaagcag aagaggatca ccctgggata 3541 tacacaggcc gatgtggggc tcaccctggg ggttctattt gggaaggtat tcagccaaac 3601 gaccatctgc cgctttgagg ctctgcagct tagcttcaag aacatgtgta agctgcggcc 3661 cttgctgcag aagtgggtgg aggaagctga caacaatgaa aatcttcagg agatatgcaa 3721 agcagaaacc ctcgtgcagg cccgaaagag aaagcgaacc agtatcgaga accgagtgag 3781 aggcaacctg gagaatttgt tcctgcagtg cccgaaaccc acactgcagc agatcagcca 3841 catcgcccag cagcttgggc tcgagaagga tgtggtccga gtgtggttct gtaaccggcg 3901 ccagaagggc aagcgatcaa gcagcgacta tgcacaacga gaggattttg aggctgctgg 3961 gtctcctttc tcagggggac cagtgtcctt tcctctggcc ccagggcccc attttggtac 4021 cccaggctat gggagccctc acttcactgc actgtactcc tcggtccctt tccctgaggg 4081 ggaagccttt ccccctgtct ctgtcaccac tctgggctct cccatgcatt caaactgaga 4141 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac 4201 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 4261 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 4321 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 4381 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 4441 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 4501 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc 4561 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 4621 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 4681 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 4741 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag 4801 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga 4861 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt 4921 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 4981 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca 5041 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct 5101 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt 5161 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt 5221 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc 5281 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 5341 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 5401 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc 5461 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 5521 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 5581 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 5641 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 5701 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 5761 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 5821 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 5881 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 5941 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 6001 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 6061 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 6121 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 6181 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 6241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 6301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 6361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 6421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct 6481 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg 6541 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag 6601 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt 6661 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg 6721 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg 6781 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg 6841 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc 6901 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc 6961 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac 7021 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 7081 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 7141 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 7201 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 7261 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 7321 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 7381 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 7441 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 7501 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 7561 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 7621 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 7681 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 7741 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 7801 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 7861 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7921 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7981 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 8041 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 8101 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 8161 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 8221 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 8281 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 8341 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 8401 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 8461 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 8521 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 8581 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 8641 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 8701 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 8761 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 8821 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 8881 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 8941 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 9001 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 9061 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 9121 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 9181 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 9241 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 9301 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 9361 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 9421 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga c //