pActXYN vector (V009592)

LOCUS       40924_3946        4895 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pActXYN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Vickers CE, Xue GP, Gresshoff PM.
  TITLE     A synthetic xylanase as a novel reporter in plants
  JOURNAL   Plant Cell Rep. 22 (2), 135-140 (2003)
  PUBMED    12845475
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Vickers CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2003) ARC Centre for Integrative Legume Research, 
            The University of Queensland, Room 213, John Hines Building (69), 
            St. Lucia, QLD 4072, Australia
REFERENCE   3  (bases 1 to 4895)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4895)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2003"; volume: "22"; issue: "2"; pages: "135-140"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-OCT-2003) ARC Centre for Integrative Legume Research, The 
            University of Queensland, Room 213, John Hines Building (69), St. 
            Lucia, QLD 4072, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2426..3259
                     /label=Act1 promoter
                     /note="rice actin promoter"
     CDS             3262..3279
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        complement(join(4894..4895,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"