LOCUS       40924_3946        4895 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pActXYN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Vickers CE, Xue GP, Gresshoff PM.
  TITLE     A synthetic xylanase as a novel reporter in plants
  JOURNAL   Plant Cell Rep. 22 (2), 135-140 (2003)
  PUBMED    12845475
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Vickers CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2003) ARC Centre for Integrative Legume Research, 
            The University of Queensland, Room 213, John Hines Building (69), 
            St. Lucia, QLD 4072, Australia
REFERENCE   3  (bases 1 to 4895)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4895)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2003"; volume: "22"; issue: "2"; pages: "135-140"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-OCT-2003) ARC Centre for Integrative Legume Research, The 
            University of Queensland, Room 213, John Hines Building (69), St. 
            Lucia, QLD 4072, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2426..3259
                     /label=Act1 promoter
                     /note="rice actin promoter"
     CDS             3262..3279
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        complement(join(4894..4895,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatctga tatcatcggg aattcgatat
     2401 caacctggcg gccgcactag catactcgag gtcattcata tgcttgagaa gagagtcggg
     2461 atagtccaaa ataaaacaaa ggtaagatta cctggtcaaa agtgaaaaca tcagttaaaa
     2521 ggtggtataa agtaaaatat cggtaataaa aggtggccca aagtgaaatt tactcttttc
     2581 tactattata aaaattgagg atgttttgtc ggtactttga tacgtcattt ttgtatgaat
     2641 tggtttttaa gtttattcgc gatttggaaa tgcatatctg tatttgagtc ggtttttaag
     2701 ttcgttgctt ttgtaaatac agagggattt gtataagaaa tatctttaaa aaacccatat
     2761 gctaatttga cataattttt gagaaaaata tatattcagg cgaattccac aatgaacaat
     2821 aataagatta aaatagcttg cccccgttgc agcgatgggt attttttcta gtaaaataaa
     2881 agataaactt agactcaaaa catttacaaa aacaacccct aaagtcctaa agcccaaagt
     2941 gctatgcacg atccatagca agcccagccc aacccaaccc aacccaaccc accccagtgc
     3001 agccaactgg caaatagtct ccacccccgg cactatcacc gtgagttgtc cgcaccaccg
     3061 cacgtctcgc agccaaaaaa aaaaaaagaa agaaaaaaaa gaaaaagaaa aacagcaggt
     3121 gggtccgggt cgtgggggcc ggaaaagcga ggaggatcgc gagcagcgac gaggcccggc
     3181 cctccctccg cttccaaaga aacgcccccc atcgccacta tatacatacc cccccctctc
     3241 ctcccatccc cccaacccta ccaccaccac caccaccacc tcctcccccc tcgctgccgg
     3301 acgacgagct cctcccccct ccccctccgc cgccgccggt aaccaccccg cccctctcct
     3361 ctttctttct ccgttttttt tttcgtctcg gtctcgatct ttggccttgg tagtttgggt
     3421 gggcgagagc ggcttcgtcg cccagatcgg tgcgcgggag gggcgggatc tcgcggctgg
     3481 cgtctccggg cgtgagtcgg cccggatcct cgcggggaat ggggctctcg gatgtagatc
     3541 tgcgatccgc cgttgttggg ggagatgatg gggggtttaa aatttccgcc atgctaaaca
     3601 agatcaggaa gaggggaaaa gggcactatg gtttatattt ttatatattt ctgctgcttc
     3661 gtcaggctta gatgtgctag atctttcttt cttctttttg tggtagaatt tgaatccctc
     3721 agcattgttc atcggtagtt tttcttttca tgatttgtga caaatgcagc ctcgtgcgga
     3781 gcttttttgt aggtagacca tggctagcaa cgggaagaag ttcaccgtcg ggaacggcca
     3841 aaaccaacac aagggcgtca acgacggctt cagctacgag atttggctcg acaacaccgg
     3901 tggcaacggc tccatgaccc tcggtagcgg cgcaacgttc aaggcagagt ggaacgcagc
     3961 cgtgaaccgc ggcaacttcc tggcacggag gggcctcgac ttcgggagcc aaaagaaggc
     4021 caccgactac gactacatcg gcctggacta cgcagccacc tacaagcaga ccgccagcgc
     4081 aagcggcaac tccaggctct gcgtgtacgg ctggttccag aacaggggtc tgaacggcgt
     4141 gccactcgtc gagtactaca tcatcgagga ctgggtcgac tgggtgccag acgcacaagg
     4201 caagatggtc accatcgacg gggcacagta caagatcttc cagatggacc acactggccc
     4261 aaccatcaac ggtggcagcg agaccttcaa gcaatacttc tccgtcaggc aacagaagag
     4321 gacctccggg cacatcaccg tctccgacca cttcaaggag tgggccaagc aaggctgggg
     4381 tatcggcaac ctctacgagg tggcactgaa cgccgagggg tggcaaagct ccggtgtggc
     4441 tgacgtcacc ctgctggacg tgtacaccac tccaaagggc tcgagcccag ccacctcagc
     4501 cgcaccacgc tgaagcttac gtatgatccc cgggctgcga ggagtctggt ggcaactaag
     4561 ccgtcatcgt catatatagc ctcgtttaat tgttcatctc tgattcgatg atgtctccca
     4621 ccttgtttcg tgtgttccca gtttgttcat cgtcttttga ttttaccggc cgtgctctgc
     4681 ttttgttttt gtttcacctg atctctctct gacttgatgt aagagtggta tctgctacga
     4741 ctatatgttg ttgggtgagg catatgtgaa tgaaatctat gaaagctccg gctatatata
     4801 tttatacaaa gggtatgaga tggatgtgaa tcctagagca tatgtgtcca acaatcaatt
     4861 cgtcgagggg gagcttcagc tgctcgatcg agttc
//
