pACTIN-LUC vector (V009593)

LOCUS       40924_3936        7421 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pACTIN-LUC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Zieler H, Huynh CQ.
  TITLE     Intron-dependent stimulation of marker gene expression in cultured 
            insect cells
  JOURNAL   Insect Mol. Biol. 11 (1), 87-95 (2002)
  PUBMED    11841506
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Zieler H, Huynh CQ.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2001) Medical Entomology Section, Laboratory of 
            Parasitic Diseases, National Institutes of Health, 4 Center Drive 
            MSC 0425, Bethesda, MD 20892-0425, USA
REFERENCE   3  (bases 1 to 7421)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7421)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Insect Mol.
            Biol."; date: "2002"; volume: "11"; issue: "1"; pages: "87-95"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2001) Medical Entomology Section, Laboratory of Parasitic 
            Diseases, National Institutes of Health, 4 Center Drive MSC 0425, 
            Bethesda, MD 20892-0425, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        140..2649
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2780..4429
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    4598..4703
                     /note="Heliothis virescens p63 chaperonin gene double 
                     poly-adenylation sequence"
     CDS             5620..6477
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6651..7239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"