LOCUS       40924_3936        7421 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pACTIN-LUC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Zieler H, Huynh CQ.
  TITLE     Intron-dependent stimulation of marker gene expression in cultured 
            insect cells
  JOURNAL   Insect Mol. Biol. 11 (1), 87-95 (2002)
  PUBMED    11841506
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Zieler H, Huynh CQ.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2001) Medical Entomology Section, Laboratory of 
            Parasitic Diseases, National Institutes of Health, 4 Center Drive 
            MSC 0425, Bethesda, MD 20892-0425, USA
REFERENCE   3  (bases 1 to 7421)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7421)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Insect Mol.
            Biol."; date: "2002"; volume: "11"; issue: "1"; pages: "87-95"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2001) Medical Entomology Section, Laboratory of Parasitic 
            Diseases, National Institutes of Health, 4 Center Drive MSC 0425, 
            Bethesda, MD 20892-0425, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        140..2649
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2780..4429
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    4598..4703
                     /note="Heliothis virescens p63 chaperonin gene double 
                     poly-adenylation sequence"
     CDS             5620..6477
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6651..7239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact
       61 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat
      121 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca
      181 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc
      241 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat
      301 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag
      361 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc
      421 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc
      481 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg
      541 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt
      601 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc
      661 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt
      721 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt
      781 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat
      841 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt
      901 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa
      961 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg
     1021 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg
     1081 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc
     1141 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc
     1201 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt
     1261 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact
     1321 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc
     1381 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat
     1441 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg
     1501 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca
     1561 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt
     1621 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag
     1681 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct
     1741 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt
     1801 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca
     1861 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc
     1921 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct
     1981 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta
     2041 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa
     2101 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat
     2161 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac
     2221 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac
     2281 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga
     2341 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt
     2401 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg
     2461 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc
     2521 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc
     2581 ttgtgctgtg tggatactcc tcccgacaca aagccgttcc atcagccagc agtggtctaa
     2641 tccagagacc ccggatctcg ttaacttcta gagggtacca ctgcaggtcg actctagagg
     2701 atccgagctt ggaattcctt tgtgttacat tcttgaatgt cgctcgcagt gacattagca
     2761 ttccggtact gttggtaaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt
     2821 ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
     2881 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc
     2941 ggaatacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
     3001 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
     3061 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
     3121 gctcaacagt atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca
     3181 aaaaattttg aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc
     3241 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
     3301 cggttttaat gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact
     3361 gataatgaat tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac
     3421 tgcctgcgtc agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
     3481 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
     3541 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt
     3601 acgatccctt caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt
     3661 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
     3721 tgggggcgca cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc
     3781 agggatacga caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     3841 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     3901 ggatctggat accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg
     3961 acctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     4021 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     4081 agttgaccgc ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt
     4141 ggaatcgata ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga
     4201 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     4261 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     4321 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     4381 aatcagagag atcctcataa aggccaagaa gggcggaaag tccaaattgt aaaatgtaac
     4441 tgtattcagc gatgacgaaa ttcttagcta ttgtaatatt atatgcaaat tgatgaatgg
     4501 taattttgta attgtgggtc actgtactat tttaacgaat aataaaatca ggtataggta
     4561 actaaaaagg aattcgagct cgatcgatac acgtgctgta cacatttact ttaaatttaa
     4621 taaaatttac tttagccgtt gtccgataat tcttatattt aatttaaacc acctgcaagc
     4681 ttttaataaa tctatatgtt cccgggcgtt taaacgcggc cgctatgcag gattttatgt
     4741 atgaaaacgc ccaccattcc cacccttctg gggccggacg gcatgacatc gctgcgcgaa
     4801 tatgccggtt atcacggcgg tggcagcgga tttggagggc agttgcggtc gtggaaccca
     4861 ccgagtgaaa gtgtggatgc agccctgttg cccaacttta cccgtggcaa tgcccgcgca
     4921 gacgatctgg tacgcaataa cggctatgcc gccaacgcca tccagctgca tcaggatcat
     4981 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     5041 gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     5101 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     5161 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     5221 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     5281 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     5341 gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca
     5401 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     5461 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
     5521 ctcgacacgc tgcatgaaac aattcaaaca gggttctggc gtcgttctcg tactgttttc
     5581 cccaggccag tgctttagcg ttattgaaaa aggaagagta tgagtattca acatttccgt
     5641 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5701 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5761 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5821 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5881 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5941 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6001 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6061 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6121 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6181 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6241 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6301 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6361 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6421 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6481 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6541 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6781 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     6841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7261 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7321 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7381 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag a
//
