pAcMLF9 vector (V009605)

LOCUS       V009605                 9366 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009605
VERSION     V009605
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Harrison RL, Bonning BC.
  TITLE     Use of scorpion neurotoxins to improve the insecticidal activity of
            Rachiplusia ou multicapsid nucleopolyhedrovirus
  JOURNAL   Biol. Control 17, 191-201 (2000)
REFERENCE   2  (bases 1 to 9366)
  AUTHORS   Harrison RL, Bonning BC.
  TITLE     Use of proteases to improve the insecticidal activity of
            baculoviruses
  JOURNAL   Biol. Control 20, 199-209 (2001)
REFERENCE   3  (bases 1 to 9366)
  AUTHORS   Harrison RL.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2006) Insect Biocontrol Laboratory, United States
            Department of Agriculture, Agricultural Research Service, Plant
            Sciences Institute, 10300 Baltimore Avenue, Beltsville, MD 20705,
            USA
REFERENCE   4  (bases 1 to 9366)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9366)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol.
            Control 17, 191-201 (2000)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Biol.
            Control 20, 199-209 (2001)"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-OCT-2006) Insect Biocontrol Laboratory, United States Department
            of Agriculture, Agricultural Research Service, Plant Sciences
            Institute, 10300 Baltimore Avenue, Beltsville, MD 20705, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     polyA_signal    complement(2102..2236)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(2563..2887)
                     /label="basic protein promoter"
                     /note="baculovirus basic protein promoter, which allows
                     expression during the late instead of very late phase of
                     viral infection"
     promoter        2920..3011
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             3012..3746
                     /gene="PH"
                     /label="Polyhedrin"
                     /note="Polyhedrin from Autographa californica nuclear
                     polyhedrosis virus. Accession#: P04871"
     misc_recomb     3662..5120
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(6260..6276)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6489..6944
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7226..7330
                     /label="AmpR promoter"
     CDS             7331..8188
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8362..8950
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9238..9259
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9274..9304
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9312..9328
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9336..9352
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"