LOCUS       V009605                 9366 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009605
VERSION     V009605
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Harrison RL, Bonning BC.
  TITLE     Use of scorpion neurotoxins to improve the insecticidal activity of
            Rachiplusia ou multicapsid nucleopolyhedrovirus
  JOURNAL   Biol. Control 17, 191-201 (2000)
REFERENCE   2  (bases 1 to 9366)
  AUTHORS   Harrison RL, Bonning BC.
  TITLE     Use of proteases to improve the insecticidal activity of
            baculoviruses
  JOURNAL   Biol. Control 20, 199-209 (2001)
REFERENCE   3  (bases 1 to 9366)
  AUTHORS   Harrison RL.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2006) Insect Biocontrol Laboratory, United States
            Department of Agriculture, Agricultural Research Service, Plant
            Sciences Institute, 10300 Baltimore Avenue, Beltsville, MD 20705,
            USA
REFERENCE   4  (bases 1 to 9366)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9366)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol.
            Control 17, 191-201 (2000)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Biol.
            Control 20, 199-209 (2001)"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-OCT-2006) Insect Biocontrol Laboratory, United States Department
            of Agriculture, Agricultural Research Service, Plant Sciences
            Institute, 10300 Baltimore Avenue, Beltsville, MD 20705, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     polyA_signal    complement(2102..2236)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(2563..2887)
                     /label="basic protein promoter"
                     /note="baculovirus basic protein promoter, which allows
                     expression during the late instead of very late phase of
                     viral infection"
     promoter        2920..3011
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             3012..3746
                     /gene="PH"
                     /label="Polyhedrin"
                     /note="Polyhedrin from Autographa californica nuclear
                     polyhedrosis virus. Accession#: P04871"
     misc_recomb     3662..5120
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(6260..6276)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6489..6944
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7226..7330
                     /label="AmpR promoter"
     CDS             7331..8188
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8362..8950
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9238..9259
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9274..9304
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9312..9328
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9336..9352
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
       61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
      121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
      181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
      241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
      301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
      361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
      421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
      481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
      541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
      601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
      661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
      721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
      781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
      841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
      901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
      961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgatc
     2101 agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     2161 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     2221 ctgcaataaa caagttccga gtttgtcaga aagcagacca aacagcggtt ggaataatag
     2281 cgagaacaga gaaatagcgg caaaaataat acccgtatca cttttgctga tatggttgat
     2341 gtcatgtagc caaatcggga aaaacgggaa gtaggctccc atgataaaaa agtaaaagaa
     2401 aaagaataaa ccgaacatcc aaaagtttgt gttttttaaa tagtacataa tggatttcct
     2461 tacgcgaaat acgggcagac atggcctgcc cggttattat tatttttgac accagaccaa
     2521 ctggtaatgg tagcgaccgg cgctcagctg gaattcagat ctgtttaaat tgtgtaattt
     2581 atgtagctgt aatttttacc ttattaatat tttttacgct ttgcattcga cgactgaact
     2641 cccaaatata tgtttaactc gtcttggtcg tttgaatttt tgttgctgtg tttcctaata
     2701 ttttccatca ccttaaatat gttattgtaa tcctcaatgt tgaacttgca attggacacg
     2761 gcatagtttt ccatagtcgt gtaaaacatg gtattggctg cattgtaata catccgactg
     2821 agcgggtacg gatctatgtg tttgagcagc ctgttcaaaa actctgcatc gtcgcaaaac
     2881 ggaatttggt acctctagag gatcgatccc ccggatctga tcatggagat aattaaaatg
     2941 ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa
     3001 aacctataaa tatgccggat tattcatacc gtcccaccat cgggcgtacc tacgtgtacg
     3061 acaacaagta ctacaaaaat ttaggtgccg ttatcaagaa cgctaagcgc aagaagcact
     3121 tcgccgaaca tgagatcgaa gaggctaccc tcgaccccct agacaactac ctagtggctg
     3181 aggatccttt cctgggaccc ggcaagaacc aaaaactcac tctcttcaag gaaatccgta
     3241 atgttaaacc cgacacgatg aagcttgtcg ttggatggaa aggaaaagag ttctacaggg
     3301 aaacttggac ccgcttcatg gaagacagct tccccattgt taacgaccaa gaagtgatgg
     3361 atgttttcct tgttgtcaac atgcgtccca ctagacccaa ccgttgttac aaattcctgg
     3421 cccaacacgc tctgcgttgc gaccccgact atgtacctca tgacgtgatt aggatcgtcg
     3481 agccttcatg ggtgggcagc aacaacgagt accgcatcag cctggctaag aagggcggcg
     3541 gctgcccaat aatgaacctt cactctgagt acaccaactc gttcgaacag ttcatcgatc
     3601 gtgtcatctg ggagaacttc tacaagccca tcgtttacat cggtaccgac tctgctgaag
     3661 aggaggaaat tctccttgaa gtttccctgg tgttcaaagt aaaggagttt gcaccagacg
     3721 cacctctgtt cactggtccg gcgtattaaa acacgataca ttgttattag tacatttatt
     3781 aagcgctaga ttctgtgcgt tgttgattta cagacaattg ttgtacgtat tttaataatt
     3841 cattaaattt ataatcttta gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt
     3901 ctttatatct gaatttaaat attaaatcct caatagattt gtaaaatagg tttcgattag
     3961 tttcaaacaa gggttgtttt tccgaaccga tggctggact atctaatgga ttttcgctca
     4021 acgccacaaa acttgccaaa tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg
     4081 ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt
     4141 catcactgtc gttagtgtac aattgactcg acgtaaacac gttaaataaa gcttggacat
     4201 atttaacatc gggcgtgtta gctttattag gccgattatc gtcgtcgtcc caaccctcgt
     4261 cgttagaagt tgcttccgaa gacgattttg ccatagccac acgacgccta ttaattgtgt
     4321 cggctaacac gtccgcgatc aaatttgtag ttgagctttt tggaattatt tctgattgcg
     4381 ggcgtttttg ggcgggtttc aatctaactg tgcccgattt taattcagac aacacgttag
     4441 aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa atctactaat ggcggcggtg
     4501 gtggagctga tgataaatct accatcggtg gaggcgcagg cggggctggc ggcggaggcg
     4561 gaggcggagg tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc tctttaggca
     4621 acacagtcgg cacctcaact attgtactgg tttcgggcgc cgtttttggt ttgaccggtc
     4681 tgagacgagt gcgatttttt tcgtttctaa tagcttccaa caattgttgt ctgtcgtcta
     4741 aaggtgcagc gggttgaggt tccgtcggca ttggtggagc gggcggcaat tcagacatcg
     4801 atggtggtgg tggtggtgga ggcgctggaa tgttaggcac gggagaaggt ggtggcggcg
     4861 gtgccgccgg tataatttgt tctggtttag tttgttcgcg cacgattgtg ggcaccggcg
     4921 caggcgccgc tggctgcaca acggaaggtc gtctgcttcg aggcagcgct tggggtggtg
     4981 gcaattcaat attataattg gaatacaaat cgtaaaaatc tgctataagc attgtaattt
     5041 cgctatcgtt taccgtgccg atatttaaca accgctcaat gtaagcaatt gtattgtaaa
     5101 gagattgtct caagctcgga tcccgcacgc cgataacaag ccttttcatt tttactacag
     5161 cattgtagtg gcgagacact tcgctgtcgt cgacgtacat gtatgctttg ttgtcaaaaa
     5221 cgtcgttggc aagctttaaa atatttaaaa gaacatctct gttcagcacc actgtgttgt
     5281 cgtaaatgtt gtttttgata atttgcgctt ccgcagtatc gacacgttca aaaaattgat
     5341 gcgcatcaat tttgttgttc ctattattga ataaataaga ttgtacagat tcatatctac
     5401 gattcgtcat ggccaccaca aatgctacgc tgcaaacgct ggtacaattt tacgaaaact
     5461 gcaaaaacgt caaaactcgg tataaaataa tcaacgggcg ctttggcaaa atatctattt
     5521 tatcgcacaa gcccactagc aaattgtatt tgcagaaaac aatttcggcg cacaatttta
     5581 acgctgacga aataaaagtt caccagttaa tgagcgacca cccaaatttt ataaaaatct
     5641 attttaatca cggttccatc aacaaccaag tgatcgtgat ggactacatt gactgtcccg
     5701 atttatttga aacactacaa attaaaggcg agctttcgta ccaacttgtt agcaatatta
     5761 ttagacagct gtgtgaagcg ctcaacgatt tgcacaagca caatttcata cacaacgaca
     5821 taaaactcga aaatgtctta tatttcgaag cacttgatcg cgtgtatgtt tgcgattacg
     5881 gattgtgcaa acacgaaaac tcacttagcg tgcacgacgg cacgttggag tattttagtc
     5941 cggaaaaaat tcgacacaca actatgcacg tttcgtttga ctggtacgcg gcgtgttaac
     6001 atacaagttg ctaaccggcg gccgacaccc atttgaaaaa agcgaagacg aaatgttgga
     6061 cttgaatagc atgaagcgtc gtcagcaata caatgacatt ggcgttttaa aacacgttcg
     6121 taacgttaac gctcgtgact ttgtgtactg cctaacaaga tacaacatag attgtagact
     6181 cacaaattac aaacaaatta taaaacatga gtttttgtcg taaaaatgcc acttgtttta
     6241 cgagtagaat tagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6301 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6361 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     6421 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     6481 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6541 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6601 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6661 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     6721 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6781 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
     6841 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6901 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     6961 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     7021 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     7081 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     7141 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     7201 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7261 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7321 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7381 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7441 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7501 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7561 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7621 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7681 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7741 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7801 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7861 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7921 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7981 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8041 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8101 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8161 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8221 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8281 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8341 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8401 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8461 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     8521 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8581 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8641 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8701 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     8761 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8821 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8881 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8941 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9001 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9061 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9121 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9181 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9241 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9301 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     9361 cgaatt
//
