pABb1p10-NA3 vector (V009649)

LOCUS       V009649                 9994 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009649
VERSION     V009649
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Baculovirus as an efficient vector for gene delivery into mosquitoes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9994)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2018) Institute of Molecular Biology, Academia
            Sinica, Nankang, Taipei 115, Taiwan
REFERENCE   3  (bases 1 to 9994)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9994)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-APR-2018) Institute of Molecular Biology, Academia Sinica,
            Nankang, Taipei 115, Taiwan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     327..1154
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     CDS             complement(1207..1923)
                     /label="EGFP"
                     /note="enhanced GFP"
     regulatory      2204..4063
                     /label="b1"
                     /note="b1"
                     /regulatory_class="promoter"
     protein_bind    4066..4090
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        4106..4215
                     /label="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     sig_peptide     4238..4294
                     /label="GP64 signal sequence"
                     /note="signal sequence from the baculovirus envelope
                     glycoprotein GP64"
     CDS             4328..5734
                     /gene="NA"
                     /label="Neuraminidase"
                     /note="Neuraminidase from Influenza A virus (strain
                     A/Tern/South Africa/1961 H5N3). Accession#: Q6XV52"
     CDS             5735..5758
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             5759..5776
                     /label="6xHis"
                     /note="6xHis affinity tag"
     misc_recomb     5793..7150
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     rep_origin      7297..7752
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8034..8138
                     /label="AmpR promoter"
     CDS             8139..8996
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      9170..9758
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"