LOCUS       V009649                 9994 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009649
VERSION     V009649
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Baculovirus as an efficient vector for gene delivery into mosquitoes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9994)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2018) Institute of Molecular Biology, Academia
            Sinica, Nankang, Taipei 115, Taiwan
REFERENCE   3  (bases 1 to 9994)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9994)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-APR-2018) Institute of Molecular Biology, Academia Sinica,
            Nankang, Taipei 115, Taiwan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     327..1154
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     CDS             complement(1207..1923)
                     /label="EGFP"
                     /note="enhanced GFP"
     regulatory      2204..4063
                     /label="b1"
                     /note="b1"
                     /regulatory_class="promoter"
     protein_bind    4066..4090
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        4106..4215
                     /label="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     sig_peptide     4238..4294
                     /label="GP64 signal sequence"
                     /note="signal sequence from the baculovirus envelope
                     glycoprotein GP64"
     CDS             4328..5734
                     /gene="NA"
                     /label="Neuraminidase"
                     /note="Neuraminidase from Influenza A virus (strain
                     A/Tern/South Africa/1961 H5N3). Accession#: Q6XV52"
     CDS             5735..5758
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             5759..5776
                     /label="6xHis"
                     /note="6xHis affinity tag"
     misc_recomb     5793..7150
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     rep_origin      7297..7752
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8034..8138
                     /label="AmpR promoter"
     CDS             8139..8996
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      9170..9758
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
       61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
      121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
      181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
      241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
      301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
      361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
      421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
      481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
      541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
      601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
      661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
      721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
      781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
      841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
      901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
      961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
     1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
     1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
     1141 aatatatagt tgctgatatt cacttgtaca gctcgtccat gccgccggtg gagtggcggc
     1201 ctattacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
     1261 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
     1321 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
     1381 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
     1441 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
     1501 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
     1561 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
     1621 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
     1681 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac
     1741 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
     1801 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
     1861 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
     1921 catggtggcg accggtagcg ctagcggatc tgacgggaat tcctgcagcc cgggggatcc
     1981 ggtaccggta tgaacacgac tcgaatcaga ctgatggtgt tcgtcaccag tctcggcttt
     2041 ttatagtgcg gatggccccc actctgttgc gataagaacc gagcgacgcc attggtcgat
     2101 gagtacctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt gggaggtgat
     2161 gtccaacttg atgttgacgt tgtaggcgcc gggcagccat cctgcctaca ctcaaatgtt
     2221 catcaatggg ttatccaacg aaagaaaaaa tgaagagaaa tcagccttac aagtccatac
     2281 gcttgagatt cgcatgtcgt caacaactat tcagcagtaa attttgtcac ataggactta
     2341 gatatcagaa tatagttttc atatatatga caaactaatt cgtgattcat taatttggtt
     2401 aagcttattt gaaacagctg ttccaacgca ataaactaac gcggtattct caatccaaac
     2461 ggtacaccag ttatgtgatt cccaatttcc cagagcaatt gcagactccc atagtaaaat
     2521 gctcattcat tcatcatcac acacgttccg ctgaatggta gacaacagtg gcgccaaaac
     2581 aaaaacagag cgaggttcat aaatggcagc tgcgcgctct cgactcgacg agcagcgcga
     2641 gctaagtgga attcaccgac ggcagccaaa ctgattagct tctaaaaaag tcaaactttt
     2701 catattttat tgaatggtaa ccaacaaaaa gattggaaat aagcaaaacc cttgcaatta
     2761 aaaattgaaa ccacgcactt aaaaataaaa caagcagaat aagtaataag ctttgaacta
     2821 tataaaaaac ggttaaaaac aactttaagt aagtgaatat gtatgatttc aattatcagc
     2881 tttcaaatac agtaatccgg gtcaatttga tcactatttt ataacttttt gctgtgttgt
     2941 ccttcggaat tcaattattg tcaaataaat ttcggcaaaa ccagaactcc attgatcttt
     3001 atctgtccat gtaatcgatt tttcatgaaa taaaatttaa catactataa aaatagtttt
     3061 aatatccaaa attagaatga cgcactggag cgaaattgat catcatataa aaaagcaggt
     3121 ttaagagtga aaatttccct atctactaaa tttgggtctc ctgaatcaga gtatgatgtc
     3181 aaaatcttac aactctagta tttgatcata ttattgcaaa atcaaacttt gaaaatctgc
     3241 ctaatacgcc taaatatagg caatttccct taaaataagc acattatttg agaataaacg
     3301 attttatgag caaaacacta tactttttat actatatgat atcaaaaatg agtttagcag
     3361 ttcatactaa aggtaacaca acattttaaa cacttatagt ttacatgtag gcatggcacc
     3421 agtggattgt ttagcatcga catttccaac tgtattgctt acaccttcaa aaattgtgaa
     3481 tatttataag caaaactgac acttattaaa atgaaatagt ttcaaatcat cattaggcac
     3541 ctataaactt gaaaacatgg aatgtgtttg gtgccaacaa acccttcagc atccttggga
     3601 ggaaatgttt ttcggtaccg acctgatcgg attcgcccca gtgatcaatt tgcccccggt
     3661 taacggtagc attttttgtt attcttattt ttttataatg tagtttttaa ttgcgaattt
     3721 ttcattgcaa ttttttgcga atttttcgat gaaaaaatat tttgaaatgc tgtttttttt
     3781 tttaataaca aatttctact gagctctatt ttttagttat tgtattgact gctaaaggct
     3841 aagcctctga tggttatcct tttcttaggg catgagaaca tttttcccgt tcaccgatcg
     3901 ttggtgcggt gatcgcctgc tcggtgattc ccggtcgtct ttggtcgcgc ctccctttct
     3961 ttacgcaaat aaaggaccag gcaaggacta taaaaccaag tcactccaag caaatcggtt
     4021 atcagttagc aaacagcgaa ccgtaagtca cctattccag tgaggggaat tgtgagcgga
     4081 taacaattcc ccggagttaa tccgggacct ttaattcaac ccaacacaat atattatagt
     4141 taaataagaa ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac
     4201 attttattta caatcaaagg agatatacca tgccaccatg gtctccgcta tcgtcttgta
     4261 cgtcttgttg gctgccgccg ctcattccgc tttcatctgt atgatccgta accgtaacag
     4321 gcagtacatg aaccccaacc agaagatcat caccatcggt gtcgtcaaca ccaccctctc
     4381 caccatcgct ttgctgatcg gtatcggtaa cctgatcttc aacaccgtga tccacgagaa
     4441 gatcggtgac caccagaccg tcgtctaccc aacaatcacc gctccagttg ttccaaactg
     4501 ttccgacacc accatcacct acaacaacac cgtggtccac aacatcacca ctactatcat
     4561 cacagaggct gagaagcact tcaagagctc cctgcctctc tgtccattcc gtggattctt
     4621 cccattccac aaggacaacg ctatcaggct cggtgaaaac aaggacgtca tcgtcacacg
     4681 tgagccttac gtttcctgtg acaacgacaa ctgttggtcc ttcgctctcg ctcaaggagc
     4741 tctgctcgga accaaacact ctaacggaac tatcaaggac cgtacaccat accgttctct
     4801 gatcaggttc ccaatcggaa ctgcccctgt tctgggaaat tacaaggaga tctgcgttgc
     4861 ctggtcttcc tcttcttgtt tcgacggaaa ggagtggatg cacgtttgca tgacaggtaa
     4921 cgacaacgac gcctcaggac agatcatcta cgccggtaag atgactgact ctatcaagag
     4981 ctggaggaag gacatcctga ggacacagga gtctgagtgc caatgtatcg acggaacatg
     5041 tgtggttgcc gttactgacg gacctgccgc caattcagcc gatcaccgta tttactggat
     5101 tcgtgaaggt aaggttatga agtacgagaa catccctaag actaagatcc agcacctcga
     5161 ggaatgctcc tgctacgtgg acattgacgt ctactgcatc tgccgcgaca actggaaggg
     5221 ttccaaccgc ccttggatgc gcattaacaa cgaaactatc ctcgaaacag gctacgtgtg
     5281 ctctaagttc cacagcgaca caccacgccc tgccgatcct tcaacagttt catgtgactc
     5341 accttctaac gtgaacggag gtcccggtgt taaaggtttc ggtttcaaga ctggtgatga
     5401 tgtgtggctg ggtcgcacag tgtcaacttc aggccgctca ggctttgaaa ttatcaaggt
     5461 gactgaaggc tggatcaaca gccccaacca cgccaaatca gtgactcaaa ctctggtgag
     5521 caacaacgat tggtccggct acagcggcag ctttattgtg gaaaacaacg gctgtttcca
     5581 accctgcttc tacatcgaac tcattcgcgg ccgccccaat aaaaatgatg atgtgagctg
     5641 gacaagcaat agcattgtga cattttgcgg cctggataat gaacccggca gcggcaattg
     5701 gcccgatggc tccaatattg gctttatgcc caaagactac aaagacgatg acgacaagca
     5761 tcaccatcac catcactaat taattgatcc gggttattag tacatttatt aagcgctaga
     5821 ttctgtgcgt tgttgattta cagacaattg ttgtacgtat tttaataatt cattaaattt
     5881 ataatcttta gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt ctttatatct
     5941 gaatttaaat attaaatcct caatagattt gtaaaatagg tttcgattag tttcaaacaa
     6001 gggttgtttt tccgaaccga tggctggact atctaatgga ttttcgctca acgccacaaa
     6061 acttgccaaa tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg ttttgttttg
     6121 taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt catcactgtc
     6181 gttagtgtac aattgactcg acgtaaacac gttaaataaa gcttggacat atttaacatc
     6241 gggcgtgtta gctttattag gccgattatc gtcgtcgtcc caaccctcgt cgttagaagt
     6301 tgcttccgaa gacgattttg ccatagccac acgacgccta ttaattgtgt cggctaacac
     6361 gtccgcgatc aaatttgtag ttgagctttt tggaattatt tctgattgcg ggcgtttttg
     6421 ggcgggtttc aatctaactg tgcccgattt taattcagac aacacgttag aaagcgatgg
     6481 tgcaggcggt ggtaacattt cagacggcaa atctactaat ggcggcggtg gtggagctga
     6541 tgataaatct accatcggtg gaggcgcagg cggggctggc ggcggaggcg gaggcggagg
     6601 tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc tctttaggca acacagtcgg
     6661 cacctcaact attgtactgg tttcgggcgc cgtttttggt ttgaccggtc tgagacgagt
     6721 gcgatttttt tcgtttctaa tagcttccaa caattgttgt ctgtcgtcta aaggtgcagc
     6781 gggttgaggt tccgtcggca ttggtggagc gggcggcaat tcagacatcg atggtggtgg
     6841 tggtggtgga ggcgctggaa tgttaggcac gggagaaggt ggtggcggcg gtgccgccgg
     6901 tataatttgt tctggtttag tttgttcgcg cacgattgtg ggcaccggcg caggcgccgc
     6961 tggctgcaca acggaaggtc gtctgcttcg aggcagcgct tggggtggtg gcaattcaat
     7021 attataattg gaatacaaat cgtaaaaatc tgctataagc attgtaattt cgctatcgtt
     7081 taccgtgccg atatttaaca accgctcaat gtaagcaatt gtattgtaaa gagattgtct
     7141 caagctcgga tcgatcccgc acgccgataa caagcctttt catttttact acagcattgt
     7201 agtggcgaga cacttcgctg tcgtcgcctg atgcggtatt ttctccttac gcatctgtgc
     7261 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     7321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7441 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7501 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7621 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     7681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     7741 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     7801 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     7861 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7921 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7981 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8041 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8101 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8161 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8221 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8281 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8341 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8401 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8461 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8521 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8581 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8641 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8701 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8761 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8821 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8881 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8941 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9001 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9061 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9121 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9241 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9301 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9481 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9841 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9901 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9961 ctctccccgc gcgttggccg attcattaat gcag
//
