pAAV-hSyn-CRMP2-hSyn-mCherry vector (V009671)

LOCUS       V009671                 7495 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009671
VERSION     V009671
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Michel U, Muller B, Koch JC, Zhang J-N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2015) Neurology, University of Goettingen, Waldweg
            33, Goettingen, Niedersachsen 37073, Germany
REFERENCE   2  (bases 1 to 7495)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7495)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (29-JUL-2015) Neurology, University of Goettingen, Waldweg 33,
            Goettingen, Niedersachsen 37073, Germany"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    48..190
                     /label="ITR"
                     /note="ITR"
     rep_origin      complement(452..1040)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1214..2071)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2072..2176)
                     /label="AmpR promoter"
     rep_origin      complement(2508..2963)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    3064..3207
                     /label="ITR"
                     /note="ITR"
     polyA_signal    complement(3217..3424)
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3510..5225)
                     /gene="DPYSL2"
                     /label="Dihydropyrimidinase-related protein 2"
                     /note="Dihydropyrimidinase-related protein 2 from Homo
                     sapiens. Accession#: Q16555"
     regulatory      5265..5744
                     /label="human synapsin promoter (hSyn)"
                     /note="human synapsin promoter (hSyn)"
                     /regulatory_class="promoter"
     promoter        complement(5271..5718)
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kugler et al., 2003)"
     polyA_signal    complement(5782..5916)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     intron          5929..6073
                     /note="chimeric intron derived from pCI-NEO"
     intron          5932..6065
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     CDS             complement(6084..6791)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     intron          complement(6826..6959)
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     promoter        complement(6999..7446)
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kugler et al., 2003)"