LOCUS       V009671                 7495 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009671
VERSION     V009671
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Michel U, Muller B, Koch JC, Zhang J-N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2015) Neurology, University of Goettingen, Waldweg
            33, Goettingen, Niedersachsen 37073, Germany
REFERENCE   2  (bases 1 to 7495)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7495)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (29-JUL-2015) Neurology, University of Goettingen, Waldweg 33,
            Goettingen, Niedersachsen 37073, Germany"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    48..190
                     /label="ITR"
                     /note="ITR"
     rep_origin      complement(452..1040)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1214..2071)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2072..2176)
                     /label="AmpR promoter"
     rep_origin      complement(2508..2963)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    3064..3207
                     /label="ITR"
                     /note="ITR"
     polyA_signal    complement(3217..3424)
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3510..5225)
                     /gene="DPYSL2"
                     /label="Dihydropyrimidinase-related protein 2"
                     /note="Dihydropyrimidinase-related protein 2 from Homo
                     sapiens. Accession#: Q16555"
     regulatory      5265..5744
                     /label="human synapsin promoter (hSyn)"
                     /note="human synapsin promoter (hSyn)"
                     /regulatory_class="promoter"
     promoter        complement(5271..5718)
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kugler et al., 2003)"
     polyA_signal    complement(5782..5916)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     intron          5929..6073
                     /note="chimeric intron derived from pCI-NEO"
     intron          5932..6065
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     CDS             complement(6084..6791)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     intron          complement(6826..6959)
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     promoter        complement(6999..7446)
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kugler et al., 2003)"
ORIGIN
        1 tcgagggggg gcccggtacc gatatcgcgt gatcctagat ctaggaaccc ctagtgatgg
       61 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg
      121 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag
      181 tggccaaccc cccccccccc ccccctgcag cccagctgca ttaatgaatc ggccaacgcg
      241 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
      301 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
      661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
      901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1141 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1201 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1261 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1321 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     1381 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1441 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1501 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1561 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1621 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     1681 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     1741 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     1801 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     1861 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1921 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1981 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2041 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2101 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2161 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     2221 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     2281 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     2341 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     2401 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     2461 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaaacgt
     2521 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     2581 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     2641 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     2701 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     2761 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     2821 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     2881 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     2941 taatgcgccg ctacagggcg cgtcgcgcca ttcgccattc aggctacgca actgttggga
     3001 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gctgcagggg gggggggggg
     3061 ggggttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     3121 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagagaggg
     3181 agtggccaac tccatcacta ggggttccta gatctctccc cagcatgcct gctattgtct
     3241 tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta
     3301 ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct
     3361 tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc
     3421 acagtcgagg ctgatcagcg agctccaccg cggtggcggc cgctctagag tcgactggta
     3481 ccgatatcag atctatcgat gaattcttag cccaggctgg tgatgttggc acggccaccg
     3541 gggggcgcca cgatacgctg ggtggtgcgg cggggaatgt tgtcatcaat ctgagcacca
     3601 gacaaactga atccagactg gtgcaggttc cggacaggtg gggcctgctg cttggcagga
     3661 gacgtcttgg ccgaggaggc tggagtgact gtcttgggcg tcacagacac ttcacacacg
     3721 ggtccgtcat acaggccacg aggaacccct ctcagctcag ccagcctgct ccttgccttg
     3781 atacgcttgt aaacaaaatc agggaagggc ttccggggaa tgtagcgtcc agagccttcg
     3841 gtgacatgca gggtgccgtc ctccaggaca atcttcccct ggctgatgac caccagtggg
     3901 gagccgcggc actccatgcc ttcaaagatg ttgtactcga gagagctgtt gtgtgtcttg
     3961 gcagagatgg ttttaacgct gtcggggtcc cagatgacca ggtcggcatc ggatcccaca
     4021 gcaatgcggc ctttccgggg gtaaaggttg aagactttgg ctgcattggt gctggtcaca
     4081 gccacaaact ggttctcatc catcttccca gtgaccacag ccttgtccca gatgacggac
     4141 atccgctcct cagtgccatt ggtgccctcc ggaatcaggg tgaagttgtc ctttcctaca
     4201 gccttctggg cagtgttaaa cgtgcaatgg gcactgcccg tgacctggag gtctccacag
     4261 gacagcaagg agttgagaaa gtctggagtg gttggatcag ggctcaaggg tggggaggtg
     4321 acaaaggcag cagccttggc ccagttcttg ctccagtaat gggagccgtc cgttcccaag
     4381 ctggcagtga tgggctcgcc atacaccaca gttcccttct tccgtgcctg ggcgatgacc
     4441 tcagcagagc ttttgctcat caccttggtg atatacagcg ggcagttggt ctggttggcg
     4501 atggtgatgg cacgattcac ggcttcggcc tcgacctcct caggtcggct cagcacatgt
     4561 ccctcggggc ccgtgatgcc cagatccagg atcctctgct gctcctctgc aatgatgtcg
     4621 ccattttctg cgtggacttg ggctatggcg ccaatatccc ggatcacact cagtacttca
     4681 taaatctggc aatccgttag ctggaagcga tctttgaaag ccatgtacac gaggaaggaa
     4741 tttaccccgt gatccttcac aagcgcttcc atctcctcct ggatgccctt atgccactcg
     4801 ctgatgtcca catgcagaga gtagtcacag caggacttgc tgtcggccca ttccctccac
     4861 tggtcaaagg cagcgagcag gcttgtccca ggctcaggaa caacgtggtc aatgatcata
     4921 gtggttcccc cagccagggc cgccttggtt ccttggaaga aatcatcagc agacgtcatt
     4981 ccctgatcag gcatctggaa acgagtgtgg acgtcaattc ctccggggat caccatccgg
     5041 gagtgggcct cgatggtctt cactcctcct ggcacaatca gattttctcc tatttgcttg
     5101 atcaacccat cttccatgta tatgtctgca tagaacgact ggtcatcatt aacaatttta
     5161 cctcctttga tcagaagacg atcgctcgtg atgcgtggaa tatttttctt cccctgataa
     5221 gacataagct gatcccccgg tagatccttg ctagcagctt gaattctcga ctgcgctctc
     5281 aggcacgaca cgactcctcc gctgcccacc gcagactgag gcagcgctga gtcgccggcg
     5341 ccgcagcgca gatggtcgcg cccgtgcccc cctatctcgc gcctcgcgtg gtgcggtccg
     5401 gctgggccgg cggcggcgcg gacgcgacca aggtggccgg gaaggggagt ttgcggggga
     5461 ccggcgagtg acgtcagcgc gccttcagtg ctgaggcggc ggtggcgcgc gccgccaggc
     5521 gggggcgaag gcactgtccg cggtgctgaa gctggcagtg cgcacgcgcc tcgccgcatc
     5581 ctgtttcccc tccccctctc tgatagggga tgcgcaattt ggggaatggg ggttgggtgc
     5641 ttgtccagtg ggtcggggtc ggtcgtcagg taggcacccc caccccgcct catcctggtc
     5701 ctaaaaccca cttgcactca tacgcagggc cctctgcagt ctagcgaata cgcgttcaga
     5761 ctagtggatc gagtgctaga cgatccagac atgataagat acattgatga gtttggacaa
     5821 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     5881 ttatttgtaa ccattataag ctgcaataaa caagttgctc gaagtcgaca cctgtggaga
     5941 gaaaggcaaa gtggatgtca gtaagaccaa taggtgccta tcagaaacgc aagagtcttc
     6001 tctgtctcga caagcccagt ttctatgtgg tctccttaaa cctgtcttgt aaccttgata
     6061 cttacctgcg gccggccgct ttacttgtac agctcgtcca tgccgccggt ggagtggcgg
     6121 ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc
     6181 aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg
     6241 gtcttgacct cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg
     6301 cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc
     6361 ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag
     6421 atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg
     6481 aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg
     6541 atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg
     6601 tcccaggcga agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc
     6661 tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc
     6721 tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc
     6781 ttgctcacca tggatctcgc aagcttcgaa ttctgcagtc gacacctgtg gagagaaagg
     6841 caaagtggat gtcagtaaga ccaataggtg cctatcagaa acgcaagagt cttctctgtc
     6901 tcgacaagcc cagtttctat gtggtctcct taaacctgtc ttgtaacctt gatacttacc
     6961 tgcggcccgg gatccttgct agcagcttga attctcgact gcgctctcag gcacgacacg
     7021 actcctccgc tgcccaccgc agactgaggc agcgctgagt cgccggcgcc gcagcgcaga
     7081 tggtcgcgcc cgtgcccccc tatctcgcgc ctcgcgtggt gcggtccggc tgggccggcg
     7141 gcggcgcgga cgcgaccaag gtggccggga aggggagttt gcgggggacc ggcgagtgac
     7201 gtcagcgcgc cttcagtgct gaggcggcgg tggcgcgcgc cgccaggcgg gggcgaaggc
     7261 actgtccgcg gtgctgaagc tggcagtgcg cacgcgcctc gccgcatcct gtttcccctc
     7321 cccctctctg ataggggatg cgcaatttgg ggaatggggg ttgggtgctt gtccagtggg
     7381 tcggggtcgg tcgtcaggta ggcaccccca ccccgcctca tcctggtcct aaaacccact
     7441 tgcactcata cgcagggccc tctgcaggca tgcaagctta tcgataccgt cgacc
//
