p5E-acta1 vector (V009714)

LOCUS       40924_2892        6618 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p5E-acta1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Jacoby AS, Busch-Nentwich E, Bryson-Richardson RJ, Hall TE, Berger 
            J, Berger S, Sonntag C, Sachs C, Geisler R, Stemple DL, Currie PD.
  TITLE     The zebrafish dystrophic mutant softy maintains muscle fibre 
            viability despite basement membrane rupture and muscle detachment
  JOURNAL   Development 136 (19), 3367-3376 (2009)
  PUBMED    19736328
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Hall TE.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2011) Australian Regenerative Medicine Institute, 
            Monash University, Building 75, Melbourne, VIC 3800, Australia
REFERENCE   3  (bases 1 to 6618)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6618)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development"; date: "2009"; volume: "136"; issue: "19"; pages: 
            "3367-3376"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-SEP-2011) Australian Regenerative Medicine Institute, Monash 
            University, Building 75, Melbourne, VIC 3800, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /label=attL4
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      689..4663
                     /label=zebrafish skeletal muscle alpha actin promoter
                     /note="zebrafish skeletal muscle alpha actin promoter"
                     /regulatory_class="promoter"
     misc_recomb     4464..4684
                     /label=attB1
                     /note="attB1"
     protein_bind    4663..4787
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4860..4878)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4883..4899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5012..5818
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5968..6556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"