LOCUS       40924_2892        6618 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p5E-acta1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Jacoby AS, Busch-Nentwich E, Bryson-Richardson RJ, Hall TE, Berger 
            J, Berger S, Sonntag C, Sachs C, Geisler R, Stemple DL, Currie PD.
  TITLE     The zebrafish dystrophic mutant softy maintains muscle fibre 
            viability despite basement membrane rupture and muscle detachment
  JOURNAL   Development 136 (19), 3367-3376 (2009)
  PUBMED    19736328
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Hall TE.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2011) Australian Regenerative Medicine Institute, 
            Monash University, Building 75, Melbourne, VIC 3800, Australia
REFERENCE   3  (bases 1 to 6618)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6618)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development"; date: "2009"; volume: "136"; issue: "19"; pages: 
            "3367-3376"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-SEP-2011) Australian Regenerative Medicine Institute, Monash 
            University, Building 75, Melbourne, VIC 3800, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /label=attL4
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      689..4663
                     /label=zebrafish skeletal muscle alpha actin promoter
                     /note="zebrafish skeletal muscle alpha actin promoter"
                     /regulatory_class="promoter"
     misc_recomb     4464..4684
                     /label=attB1
                     /note="attB1"
     protein_bind    4663..4787
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4860..4878)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4883..4899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5012..5818
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5968..6556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggc acgcgcagat tccgtgtggg cctggtaata
      721 tcttaccatt ttaagctatt ttaagattag ggaaaaaaat taaataaaac tactttaaaa
      781 ttactttcct taaaaagcaa catatttatt tagtttttgt ttaactggct caagattgta
      841 atattttact tttacatgtt ttttttaagt acctaaatgt gttcaaaagt aaagtatgtt
      901 attgtttttt tttttttaat gttagctaag ctttaaattg taatatatta cttttaaaag
      961 tgacttttta aaaatgtaaa taaaaaagta actataaatg tgtaatgcat gttatttctg
     1021 tgggtttttt aattttcatc tcaactgggc ttacaatttt aacataataa aaatcaaatc
     1081 aaatcaaatc aggcccttat ttgtcacata cactcttgta tactgaaata aactaaatgt
     1141 tgagaaaaat ttaaatacac caaaataacg catacctttt ttgtaattac ctaagtaaaa
     1201 catttagttc cttttttaag tgactcaaga ttgtaatcta ttaattgtaa aagataaaac
     1261 tagtgatcaa tctgacgtgc ttctatgcag cctgtgagat taattgtgat gtttaattat
     1321 taaacaattt aaatggatca aacatttcac atgtgcttat tgtgtccaac aaaaccagca
     1381 gaagcactta aaaatattgc atgacaacat gagtttttaa tgtaaattat atatacttcc
     1441 taaacgaagt taatcatatt ttctaaaata ataatgtcat tgaaatattt aaataaatga
     1501 taasctttag ttgattcaac ataaaattga aagcaataag gacttttttt tttttacagc
     1561 ggtgaagcaa aagcmtgkaa ataatcatat aaatataata aataaataaa taaaataatg
     1621 gttcttggtg caaatatgaa cactttaaat aaatatagaa taaatatgat caataagtca
     1681 taacgacgtg ttttatgttg attataattt aaacatgttt ctaacgcaag ttttaaaaac
     1741 tgttttaaag tattatttta agttataaga tcaatttgac tcaacatttt gtagtgaaaa
     1801 tatgttgtgt aaaattaata taatatacac tataaaaatg caaggttcca cacaattcat
     1861 tcatgttgtc ccaacacaaa tcgattaagt taacaaatat atgtggattg aacataaaaa
     1921 attaagttgt cccaatgaaa tctcaagaat tgtgttgttt cagctcattt taaataagta
     1981 gttttaacaa gtaaaaaata acatattttt gagtgtgtat atatatatat ttatatatta
     2041 caaaatataa taatataaaa tgtaaaagat ataaaccatg ctacatatat ttaagagcta
     2101 ttttgcacat tacttttttg caagccattt gagtgttttt ttacaaatat atattagcta
     2161 aatatacaat atcttaaata tacaattaat cagacatgca aaaaatgcat ttaacctaca
     2221 taaaaataca tgatcaacat atgttttatg ctgattacaa ctaaatcaaa cgtaatgaaa
     2281 ttaatttata aaagcgattt aaatatgtca atattttaag ttaaaatcac ttgaaaccta
     2341 ctataaatga acttaaattt aaaaaatctc ctttaattcc aatgcagcac tgttgttttt
     2401 tcagctgagg acccccggtg cagatggttg gcccccggcc cgctattcta aggaagcctt
     2461 tcttaatttt agggcggcgc tgcatgcgct cagatcagat tcacgggtgg agagggttat
     2521 tttctctttt ttattttttc ttgcctgctc atatttgggc ctgtgtgaac cccaggctcc
     2581 gcctcggccc ccaggagccg ctcttattta aaggggatcc tcgccttcag tcttcgtccc
     2641 agtggaagca tcagtcttgt gataacaact tggtgagtcc tacagaccag cgctcgactc
     2701 taggctttaa atgcattgat ttctctggat tagctaccgg gagagtttta actttgctta
     2761 ttagtcttgt gcttgaactg ctaggtttaa agagggtttt ctctaatgca tgcatgaagc
     2821 gacagctgaa aaccagaaat agagcgtttg tcctcctgtt aaactctagc ctttctttgg
     2881 catttgtcag tccgagtctt cagctgccgg caggagattg ggtttcagta acacttgctg
     2941 agcacaaact acacgtgctt ccttcagatt taattcatta ttaatcattc tagatttaaa
     3001 tgatcttctt actcattgct gattatttta gccttgcgga gcagattaaa tgagcacgtg
     3061 tttattaaga ttgatttaaa tataattaat atttcaccac tgtagtttta aaattgcctt
     3121 ctaagtcatg gttgattttt aataggtatc ggctctaatt agattactat tttggttttt
     3181 aaatgttatt tctatttcat ttgtaggctc gtttttaatg tgattttatt agattctagt
     3241 tttaaaatca tcttttagcc ttactgagca gactgaatga acatgtttca ttaagattaa
     3301 cttcattata gttaattatt taatcattct agtggattat aaatcagctc tatttaaatt
     3361 gattgtcgtg tttatttcta tttgattttt gattattttt taaggtcgtt tttatgtgat
     3421 ttttccccga ttctaatttt aaactaattg tttgattttt atatgattcg actcttttta
     3481 aatgaattgt tttgcttttt ttattgagca tatatatata ttatacaatt tcaatcaaca
     3541 attgttgtgc tttttaaatg ttgtttctat ttcatttcta gggtcgattt tattcgattc
     3601 tagttttaaa atcgtgattt ctaactccta accttactga gcagactgaa tgaacatgtt
     3661 tcatcaagat taacttcatt atagttaatt attgaatcgg gctagtggtt tataaatcag
     3721 ctctattata aattaattgt catcattatt tctatttaat ttttattatt tttttaggat
     3781 tatttttaag ggattttttt cacgttctag ttttaaactc aatgctcatt actgctcacc
     3841 cttgctgagc agtctttcgt taagattaaa ttaattaata acccattcta gttttaaaac
     3901 agtgtcttaa ggcatagctg atattttaca agtttactat ttaatatatc tccatttctt
     3961 tttttagggt cgatttcatc tgatttgatt aatttttatt atcttcacta agctttttgt
     4021 tctaggtttt attctcagta ttattactgt tatatttgaa ctgaatttct cctcatagtt
     4081 tattcatttt atttatttat tttttgttta ttcgctattt tgtattttag tttttaatta
     4141 gaaattttat tagaatcgtt ttgaactagt ttttatgcac tctaacatta atattaaatc
     4201 ataactatta tttttattat aatttatttg tgtttatctt tttgtgtagc tttcatgtgt
     4261 ttttatttta gttttattat gtttgcattg aattttttgg actagtttat ataacgatat
     4321 acagaaaagc catgacaaca aatgttttta taatacctat taagtataag ctaatcagat
     4381 ttccactatt ctgttcaaag ttaacattat tagccattct gatggatcat ttgacatttg
     4441 tcatatgtct ttatgaacac tgctctttca gatgccactt ttaaagctgt tttttacagt
     4501 aaatgaacgt ttattattat gacattattt cctaaggcct ctagagtaca gcaggatgtg
     4561 gcgtgtgcat tactgtgtaa ttatgctgac tgtaaatgtg catttataga ctgtcagcat
     4621 gtctgactgt gcgctgttgt gatttgtcct gcacagacca aggcaagttt gtacaaaaaa
     4681 gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     4741 aaacagacta cataatactg taaaacacaa catatgcagt cactatgaat caactactta
     4801 gatggtatta gtgacctgta gaattcgagc tctagagctg cagggcggcc gcgatatccc
     4861 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     4921 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     4981 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     5041 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     5101 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     5161 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     5221 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     5281 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     5341 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     5401 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     5461 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     5521 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     5581 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     5641 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     5701 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     5761 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     5821 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     5881 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     5941 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6001 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6061 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6121 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6181 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6241 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6301 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     6361 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6421 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6481 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6541 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6601 gccttttgct cacatgtt
//
