Basic Vector Information
- Vector Name:
- p3E-zfRab18a-R93del
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3253 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Nixon SJ, Hall TE, Parton RG.
p3E-zfRab18a-R93del vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
p3E-zfRab18a-R93del vector Sequence
LOCUS 40924_2652 3253 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector p3E-zfRab18a-R93del, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3253)
AUTHORS Nixon SJ, Hall TE, Parton RG.
TITLE Rab18 in zebrafish
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3253)
AUTHORS Hall TE, Parton RG.
TITLE Direct Submission
JOURNAL Submitted (13-JUN-2014) Institute for Molecular Bioscience,
University of Queensland, 306 Carmody Road, St Lucia, QLD 4067,
Australia
REFERENCE 3 (bases 1 to 3253)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3253)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-JUN-2014) Institute for Molecular Bioscience, University of
Queensland, 306 Carmody Road, St Lucia, QLD 4067, Australia"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..3253
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(268..295)
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(387..473)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 537..553
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(624..748)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
CDS 747..1361
/codon_start=1
/product="Rab18a"
/label=Rab18a
/note="with R93del mutation; derived from zebrafish"
/protein_id="AIO08314.1"
/translation="MNDDILTTLKILIIGESGVGKSSLLLRFTDDTFDAEIGATIGVDF
KVKTLAVDGNRAKLAIWDTAGQERFRTLTPSYYRGAQGVILVYDVSRETFAKLDNWLSE
LETYCTRNDLVKMLVGNKIDKDDREIEREEGLKFARKHSMLFIESSAKTRDGVQCAFEE
LVEKILQTPGLWESMHKSRGVALSELSESSQGGCGAYCSLL"
protein_bind complement(1362..1457)
/label=attL3
/note="recombination site for the Gateway(R) LR reaction"
promoter complement(1495..1513)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1518..1534)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 1647..2453
/codon_start=1
/label=KanR
/note="aminoglycoside phosphotransferase"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 2603..3191
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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