LOCUS       40924_2652        3253 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p3E-zfRab18a-R93del, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Nixon SJ, Hall TE, Parton RG.
  TITLE     Rab18 in zebrafish
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Hall TE, Parton RG.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2014) Institute for Molecular Bioscience, 
            University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, 
            Australia
REFERENCE   3  (bases 1 to 3253)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3253)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-JUN-2014) Institute for Molecular Bioscience, University of 
            Queensland, 306 Carmody Road, St Lucia, QLD 4067, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..748)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             747..1361
                     /codon_start=1
                     /product="Rab18a"
                     /label=Rab18a
                     /note="with R93del mutation; derived from zebrafish"
                     /protein_id="AIO08314.1"
                     /translation="MNDDILTTLKILIIGESGVGKSSLLLRFTDDTFDAEIGATIGVDF
                     KVKTLAVDGNRAKLAIWDTAGQERFRTLTPSYYRGAQGVILVYDVSRETFAKLDNWLSE
                     LETYCTRNDLVKMLVGNKIDKDDREIEREEGLKFARKHSMLFIESSAKTRDGVQCAFEE
                     LVEKILQTPGLWESMHKSRGVALSELSESSQGGCGAYCSLL"
     protein_bind    complement(1362..1457)
                     /label=attL3
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1495..1513)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1518..1534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1647..2453
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2603..3191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtggtga atgatgatat attgaccact ctgaaaatac
      781 tgatcattgg cgaaagtgga gtaggcaaat ccagtcttct tttgagattt actgatgata
      841 catttgatgc agaaattggt gcaacaatag gtgtggattt caaggtgaaa acgctggctg
      901 tagatggaaa ccgagcaaaa ctagcaatct gggatacagc aggtcaagaa cgttttcgga
      961 ctttaacacc cagttactac agaggagctc agggtgtcat tctggtgtat gatgtctcta
     1021 gggagacatt tgctaagctg gataactggt tgagtgagct ggagacgtac tgtacaagaa
     1081 atgatcttgt caagatgcta gtgggaaaca aaatcgataa ggatgatcgt gagattgagc
     1141 gagaggaggg cctgaagttc gcaagaaaac actccatgct tttcatagag tccagtgcta
     1201 aaacaaggga tggtgtccag tgtgcctttg aggagctggt ggagaaaatc ctgcagactc
     1261 caggcttgtg ggagagtatg cataagtcaa gaggagttgc cctgtctgaa ctctctgaaa
     1321 gtagtcaagg aggctgcggg gcgtactgct cacttctcta acaactttat tatacaaagt
     1381 tggcattata aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     1441 aaataaaatc attatttgga gctccatggt agcgttaacg cggccgcgat atcccctata
     1501 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     1561 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     1621 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
     1681 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     1741 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
     1801 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     1861 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     1921 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
     1981 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     2041 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     2101 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     2161 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     2221 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     2281 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     2341 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     2401 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     2461 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa
     2521 gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     2581 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2641 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2701 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2761 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2821 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2881 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2941 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     3001 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3061 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3121 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3181 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3241 ttgctcacat gtt
//
