Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V009750 | p35S-GFP | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
p35S-GFP carries the gene for the green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria. p35S-GFP was constructed by replacing the GUS coding sequence of pBI221 with a functional GFP gene, thereby placing the GFP gene under the control of the CaMV 35S promoter. Protoplasts were viewed by incident-light fluorescence microscopy 24h after electroporation. 20-60% of the protoplasts emitted an intense green light when illuminated with blue (450-490 nm) light.
- Vector Name:
- p35S-GFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4518 bp
- Type:
- Plant GFP
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Niedz RP, Sussman MR, Satterlee JS.
- Promoter:
- CaMV35S(long)
- Growth Strain(s):
- stbl3
- Growth Temperature:
- 37℃
p35S-GFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Niedz RP, Sussman MR, Satterlee JS. Green fluorescent protein: an in vivo reporter of plant gene expression. Plant Cell Rep. 1995, Apr;14(7):403-6.
- Lambardi M, Lachance D, Séguin A, Charest PJ. Evaluation of microprojectile-mediated DNA delivery and reporter genes for genetic transformation of the Mediterranean cypress (Cupressus sempervirens L.). Plant Cell Rep. 1998 Dec;18(3-4):198-202.
p35S-GFP vector Sequence
LOCUS 40924_2622 4518 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector p35S-GFP, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4518)
AUTHORS Niedz RP, Sussman MR, Satterlee JS.
TITLE Green fluorescent protein: an in vivo reporter of plant gene
expression
JOURNAL Plant Cell Rep. 14 (7), 403-406 (1995)
PUBMED 24185445
REFERENCE 2 (bases 1 to 4518)
AUTHORS Kitts PA.
TITLE CLONTECH Vectors On Disk version 1.3
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 4518)
AUTHORS Kitts PA.
TITLE Direct Submission
JOURNAL Submitted (05-JUN-1995) Paul A. Kitts, CLONTECH Laboratories, Inc.,
4030 Fabian Way, Palo Alto, CA 94303, USA
REFERENCE 4 (bases 1 to 4518)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 4518)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell
Rep."; date: "1995"; volume: "14"; issue: "7"; pages: "403-406"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(05-JUN-1995) Paul A. Kitts, CLONTECH Laboratories, Inc., 4030
Fabian Way, Palo Alto, CA 94303, USA"
COMMENT SGRef: number: 4; type: "Journal Article"
COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 4030
Fabian Way, Palo Alto, CA 94303, USA. To place an order call (415)
424-8222 or (800) 662-2566, extension 1. International customers,
please contact your local distributor. For technical information,
call (415) 424-8222 or (800) 662-2566, extension 3. This sequence
has been compiled from information in the sequence databases,
published literature and other sources, together with partial
sequences obtained by CLONTECH. If you suspect there is an error in
this sequence, please contact CLONTECH's Technical Service
Department at (415) 424-8222 or (800) 662-2566, extension 3 or
E-mail TECH@CLONTECH.COM.
FEATURES Location/Qualifiers
source 1..4518
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 512..857
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 896..1609
/codon_start=1
/label=GFP
/note="Aequorea victoria green fluorescent protein"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
terminator 1629..1881
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(1890..1906)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2380..2484
/label=AmpR promoter
CDS 2485..3342
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3516..4104
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4392..4413
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4428..4458
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4466..4482
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4490..4506
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"