LOCUS       40924_2622        4518 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p35S-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Niedz RP, Sussman MR, Satterlee JS.
  TITLE     Green fluorescent protein: an in vivo reporter of plant gene 
            expression
  JOURNAL   Plant Cell Rep. 14 (7), 403-406 (1995)
  PUBMED    24185445
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disk version 1.3
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4518)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUN-1995) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            4030 Fabian Way, Palo Alto, CA 94303, USA
REFERENCE   4  (bases 1 to 4518)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4518)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "1995"; volume: "14"; issue: "7"; pages: "403-406"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (05-JUN-1995) Paul A. Kitts, CLONTECH Laboratories, Inc., 4030 
            Fabian Way, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 4030 
            Fabian Way, Palo Alto, CA 94303, USA. To place an order call (415) 
            424-8222 or (800) 662-2566, extension 1. International customers, 
            please contact your local distributor. For technical information, 
            call (415) 424-8222 or (800) 662-2566, extension 3. This sequence 
            has been compiled from information in the sequence databases, 
            published literature and other sources, together with partial 
            sequences obtained by CLONTECH. If you suspect there is an error in 
            this sequence, please contact CLONTECH's Technical Service 
            Department at (415) 424-8222 or (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..4518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        512..857
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             896..1609
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1629..1881
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1890..1906)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2380..2484
                     /label=AmpR promoter
     CDS             2485..3342
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3516..4104
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4392..4413
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4428..4458
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4466..4482
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4490..4506
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
       61 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      121 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      181 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      241 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      301 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      361 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      421 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      481 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      541 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      601 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      661 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
      721 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
      781 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
      841 ttcatttgga gagaacacgg gggactctag aggatccata gatctgataa caaagatgag
      901 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      961 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     1021 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     1081 tactttctct tatggtgttc aatgcttttc aagataccca gatcatatga aacagcatga
     1141 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     1201 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1261 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     1321 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     1381 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     1441 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     1501 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     1561 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aagagctcga
     1621 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1681 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1741 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1801 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1861 tgtcatctat gttactagat cgggaattca ctggccgtcg ttttacaacg tcgtgactgg
     1921 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     1981 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     2041 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     2101 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     2161 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     2221 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     2281 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     2341 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     2401 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2461 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     2521 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2581 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2641 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2701 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2761 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2821 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2881 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2941 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3001 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3061 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3121 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3181 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     3241 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3301 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     3361 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     3421 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3481 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3541 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3601 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3661 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3721 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3781 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3841 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3901 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3961 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4021 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4081 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4141 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4201 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4261 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4321 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     4381 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     4441 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     4501 tatgaccatg attacgcc
//
