Basic Vector Information
- Vector Name:
- p2RLGW7
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6885 bp
- Type:
- Gateway vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Shen J, Liu J, Xie K, Xing F, Xiong F, Xiao J, Li X, Xiong L.
- Promoter:
- CaMV 35S
p2RLGW7 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
p2RLGW7 vector Sequence
LOCUS 40924_2542 6885 bp DNA circular SYN 18-DEC-2018
DEFINITION Gateway vector p2RLGW7, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6885)
AUTHORS Shen J, Liu J, Xie K, Xing F, Xiong F, Xiao J, Li X, Xiong L.
TITLE Translational repression by a miniature inverted-repeat transposable
element in the 3' untranslated region
JOURNAL Nat Commun 8, 14651 (2017)
PUBMED 28256530
REFERENCE 2 (bases 1 to 6885)
AUTHORS Shen J, Liu J, Xie K, Xing F, Xiong F, Xiao J, Li X, Xiong L.
TITLE Direct Submission
JOURNAL Submitted (09-JAN-2017) Huazhong Agricultural University, National
Key Laboratory of Crop Genetic Improvement, Shizishan Street No. 1,
Wuhan, Hubei 430070, China
REFERENCE 3 (bases 1 to 6885)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6885)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
8, 14651 (2017)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(09-JAN-2017) Huazhong Agricultural University, National Key
Laboratory of Crop Genetic Improvement, Shizishan Street No. 1,
Wuhan, Hubei 430070, China"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6885
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter complement(25..43)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(50..66)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 207..662
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 740..844
/label=AmpR promoter
CDS 845..1702
/label=AmpR
/note="beta-lactamase"
rep_origin 1876..2464
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2752..2773
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2788..2818
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2826..2842
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2850..2866
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 2884..2902
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
promoter 3562..3906
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 3999..4931
/label=Rluc
/note="luciferase from the anthozoan coelenterate Renilla
reniformis (sea pansy)"
protein_bind 4941..5065
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 5090..5120
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 5174..5830
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 6175..6477
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(6521..6645)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
regulatory 6655..6880
/label=35S terminator
/note="35S terminator"
/regulatory_class="terminator"
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