LOCUS       40924_2542        6885 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway vector p2RLGW7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Shen J, Liu J, Xie K, Xing F, Xiong F, Xiao J, Li X, Xiong L.
  TITLE     Translational repression by a miniature inverted-repeat transposable
            element in the 3' untranslated region
  JOURNAL   Nat Commun 8, 14651 (2017)
  PUBMED    28256530
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Shen J, Liu J, Xie K, Xing F, Xiong F, Xiao J, Li X, Xiong L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2017) Huazhong Agricultural University, National 
            Key Laboratory of Crop Genetic Improvement, Shizishan Street No. 1, 
            Wuhan, Hubei 430070, China
REFERENCE   3  (bases 1 to 6885)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6885)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            8, 14651 (2017)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-2017) Huazhong Agricultural University, National Key 
            Laboratory of Crop Genetic Improvement, Shizishan Street No. 1, 
            Wuhan, Hubei 430070, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(25..43)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(50..66)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      207..662
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        740..844
                     /label=AmpR promoter
     CDS             845..1702
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1876..2464
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2752..2773
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2788..2818
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2826..2842
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2850..2866
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2884..2902
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        3562..3906
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3999..4931
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
     protein_bind    4941..5065
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5090..5120
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             5174..5830
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             6175..6477
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(6521..6645)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      6655..6880
                     /label=35S terminator
                     /note="35S terminator"
                     /regulatory_class="terminator"
ORIGIN
        1 catgcgacgt cgggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
       61 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      121 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      181 agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
      241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      421 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
      481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
      541 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
      601 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
      661 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catcaggtgg
      721 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      781 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      841 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      901 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      961 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1021 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1081 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1141 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1201 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1261 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1321 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1381 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1441 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1501 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1561 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1621 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1681 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1741 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1801 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1861 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1921 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1981 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2041 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2101 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2161 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2221 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2281 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2341 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2401 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2461 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2521 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2581 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2641 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     2701 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2761 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2821 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2881 gctatttagg tgacactata gaatactcaa gctatgcatc caacgcgttg ggagctctcc
     2941 catatggtcg actagagcca agctgatctc ctttgccccg gagatcacca tggacgactt
     3001 tctctatctc tacgatctag gaagaaagtt cgacggagaa ggtgacgata ccatgttcac
     3061 caccgataat gagaagatta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     3121 tagagaggcc tacgcggcag gtctcatcaa gacgatctac ccgagtaata atctccagga
     3181 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat
     3241 caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat
     3301 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta agaaagtagt
     3361 tcctactgaa tcaaaggcca tggagtcaaa aattcagatc gaggatctaa cagaactcgc
     3421 cgtgaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat
     3481 cttcgtcaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
     3541 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
     3601 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
     3661 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
     3721 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
     3781 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
     3841 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
     3901 gaggactccg gtatttttac aacaatacca caacaaaaca aacaacaaac aacattacaa
     3961 tttactattc tagtcgacct gcaggcggcc gcactagtat gacttcgaaa gtttatgatc
     4021 cagaacaaag gaaacggatg ataactggtc cgcagtggtg ggccagatgt aaacaaatga
     4081 atgttcttga ttcatttatt aattattatg attcagaaaa acatgcagaa aatgctgtta
     4141 tttttttaca tggtaacgcg gcctcttctt atttatggcg acatgttgtg ccacatattg
     4201 agccagtagc gcggtgtatt ataccagacc ttattggtat gggcaaatca ggcaaatctg
     4261 gtaatggttc ttataggtta cttgatcatt acaaatatct tactgcatgg tttgaacttc
     4321 ttaatttacc aaagaagatc atttttgtcg gccatgattg gggtgcttgt ttggcatttc
     4381 attatagcta tgagcatcaa gataagatca aagcaatagt tcacgctgaa agtgtagtag
     4441 atgtgattga atcatgggat gaatggcctg atattgaaga agatattgcg ttgatcaaat
     4501 ctgaagaagg agaaaaaatg gttttggaga ataacttctt cgtggaaacc atgttgccat
     4561 caaaaatcat gagaaagtta gaaccagaag aatttgcagc atatcttgaa ccattcaaag
     4621 agaaaggtga agttcgtcgt ccaacattat catggcctcg tgaaatcccg ttagtaaaag
     4681 gtggtaaacc tgacgttgta caaattgtta ggaattataa tgcttatcta cgtgcaagtg
     4741 atgatttacc aaaaatgttt attgaatcgg acccaggatt cttttccaat gctattgttg
     4801 aaggtgccaa gaagtttcct aatactgaat ttgtcaaagt aaaaggtctt catttttcgc
     4861 aagaagatgc acctgatgaa atgggaaaat atatcaaatc gttcgttgag cgagttctca
     4921 aaaatgaaca ataaggtacc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
     4981 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     5041 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta
     5101 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccggcgagat tttcaggagc
     5161 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     5221 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     5281 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     5341 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
     5401 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     5461 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     5521 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     5581 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     5641 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     5701 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg
     5761 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     5821 gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt
     5881 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa
     5941 aagaggtgtg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg
     6001 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag
     6061 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg
     6121 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa cagggactgg tgaaatgcag
     6181 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
     6241 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg
     6301 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
     6361 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat
     6421 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
     6481 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg
     6541 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
     6601 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatat cccgcggcca
     6661 tgctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct
     6721 ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct
     6781 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
     6841 aataaaattt ctaattccta aaaccaaaat ccagtgacct gcagg
//
