p2619-EO6 vector (V009770)

MSDS

Basic Vector Information

- - Vector Name:
  - p2619-EO6
- - Antibiotic Resistance:
  - Kanamycin
- - Length:
  - 5686 bp
- - Type:
  - Shuttle vector
- - Replication origin:
  - ori
- - Source/Author:
  - Dennehy ME, Bourn W, Steele AD, Williamson A-L.

p2619-EO6 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

p2619-EO6 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_2498        5686 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector p2619-EO6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice 
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2005) Medical Virology, University of Cape Town, 
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5686)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5686)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JUL-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             121..933
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1268..1856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2029..3927
                     /label=Mycobacterial OriM region derived from pAL5000
                     /note="Mycobacterial OriM region derived from pAL5000"
     regulatory      3960..4200
                     /note="derived from Mycobacterium bovis hsp60 promoter of 
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     misc_feature    4200..4344
                     /note="similar to Mycobacterium tuberculosis 19 kDa
                     antigen"
     CDS             4355..5545
                     /note="Intermediate capsid protein VP6 from Rotavirus A
                     (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]). 
                     Accession#: B2BN53"
     terminator      5604..5650
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"

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