LOCUS       40924_2498        5686 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector p2619-EO6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice 
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2005) Medical Virology, University of Cape Town, 
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5686)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5686)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JUL-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             121..933
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1268..1856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2029..3927
                     /label=Mycobacterial OriM region derived from pAL5000
                     /note="Mycobacterial OriM region derived from pAL5000"
     regulatory      3960..4200
                     /note="derived from Mycobacterium bovis hsp60 promoter of 
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     misc_feature    4200..4344
                     /note="similar to Mycobacterium tuberculosis 19 kDa
                     antigen"
     CDS             4355..5545
                     /note="Intermediate capsid protein VP6 from Rotavirus A
                     (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]). 
                     Accession#: B2BN53"
     terminator      5604..5650
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagccaac aaagcgacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa
       61 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
      121 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
      181 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      241 tatcgcttgt atgggaagcc ccatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      301 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      361 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      421 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      481 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      541 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      601 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
      661 gaaatgcata atcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
      721 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
      781 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
      841 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
      901 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
      961 cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt
     1021 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa
     1081 agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct
     1141 ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca gcaacacctt
     1201 cttcacgagg cagacctcac tagttccact gagcgtcaga ccccgtagaa aagatcaaag
     1261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1381 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1621 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     1681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1921 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1981 ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg ccgcacgcgt gagcccacca
     2041 gctccgtaag ttcgggcgct gtgtggctcg tacccgcgca ttcaggcggc agggggtcta
     2101 acgggtctaa ggcggcgtgt acggccgcca cagcggctct cagcggcccg gaaacgtcct
     2161 cgaaacgacg catgtgttcc tcctggttgg tacaggtggt tgggggtgct cggctgtcgc
     2221 tggtgttcca ccaccagggc tcgacgggag agcgggggag tgtgcagttg tggggtggcc
     2281 cctcagcgaa atatctgact tggagctcgt gtcggaccat acaccggtga ttaatcgtgg
     2341 tctactacca agcgtgagcc acgtcgccga cgaatttgag cagctctggc tgccgtactg
     2401 gccgctggca agcgacgatc tgctcgaggg gatctaccgc caaagccgcg cgtcggccct
     2461 aggccgccgg tacatcgagg cgaacccaac agcgctggca aacctgctgg tcgtggacgt
     2521 agaccatcca gacgcagcgc tccgagcgct cagcgcccgg gggtcccatc cgctgcccaa
     2581 cgcgatcgtg ggcaatcgcg ccaacggcca cgcacacgca gtgtgggcac tcaacgcccc
     2641 tgttccacgc accgaatacg cgcggcgtaa gccgctcgca tacatggcgg cgtgcgccga
     2701 aggccttcgg cgcgccgtcg atggcgaccg cagttactca ggcctcatga ccaaaaaccc
     2761 cggccacatc gcctgggaaa cggaatggct ccactcagat ctctacacac tcagccacat
     2821 cgaggccgag ctcggcgcga acatgccacc gccgcgctgg cgtcagcaga ccacgtacaa
     2881 agcggctccg acgccgctag ggcggaattg cgcactgttc gattccgtca ggttgtgggc
     2941 ctatcgtccc gccctcatgc ggatctacct gccgacccgg aacgtggacg gactcggccg
     3001 cgcgatctat gccgagtgcc acgcgcgaaa cgccgaattt ccgtgcaacg acgtgtgtcc
     3061 cggaccgcta ccggacagcg aggtccgcgc catcgccaac agcatttggc gttggatcac
     3121 aaccaagtcg cgcatttggg cggacgggat cgtggtctac gaggccacac tcagtgcgcg
     3181 ccagtcggcc atctcgcgga agggcgcagc agcgcgcacg gcggcgagca cagttgcgcg
     3241 gcgcgcaaag tccgcgtcag ccatgcatgg aggcattgct atgagcgacg gctacagcga
     3301 cggctacagc gacggctaca accggcagcc gactgtccgc aaaaagcggc gcgtgaccgc
     3361 cgccgaaggc gctcgaatca ccggactatc cgaacgccac gtcgtccggc tcgtggcgca
     3421 ggaacgcagc gagtggctcg ccgagcaggc tgcacgccgc gaacgcatcc gcgcctatca
     3481 cgacgacgag ggccactctt ggccgcaaac ggccaaacat ttcgggctgc atctggacac
     3541 cgttaagcga ctcggctatc gggcgaggaa agagcgtgcg gcagaacagg aagcggctca
     3601 aaaggcccac aacgaagccg acaatccacc gctgttctaa cgcaattggg gagcgggtgt
     3661 cgcgggggtt ccgtgggggg ttccgttgca acgggtcgga caggtaaaag tcctggtaga
     3721 cgctagtttt ctggtttggg ccatgcctgt ctcgttgcgt gtttcgttgc gcccgttttg
     3781 aataccagcc agacgagacg gggttctacg aatcttggtc gataccaagc catttccgct
     3841 gaatatcggg gagctcaccg ccagaatcgg tggttgtggt gatgtacgtg gcgaactccg
     3901 ttgtagtgtt gtggtggcat ccgtggcgcg gccgcggtac cagatcttta aatctagaca
     3961 aggtcgaacg aggggcatga cccggtgcgg ggcttcttgc actcggcata ggcgagtgct
     4021 aagaataacg ttggcactcg cgaccggtga gtcgtaggtc gggacggtga ggccaggccc
     4081 gtcgtcgcag cgagtggcag cgaggacaac ttgagccgtc cgtcgcgggc actgcgcccg
     4141 gccagcgtaa gtagcggggt tgccgtcacc cggtgacccc cggtttcatc cccgatcgct
     4201 agatatccat ggatctgtcc tcaatgccga tggaccgcta cgacaggcaa aggagcacag
     4261 ggtgaagcgt ggactgacgg tcgcggtagc cggagccgcc attctggtcg caggtctttc
     4321 cggatgttca agcaacaagt ccacggatcc caaaatggat gtgctgtact ccatctcgag
     4381 aacactgaag gatgctaggg ataaaattgt tgagggtacg ttgtactcta acgttagtga
     4441 tcttattcag cagttcaatc agatgctggt cacaatgaat ggaaatgaat ttcaaacggg
     4501 gggaattggt aacttgccac ttcgtaattg gaactttgat tttggactac taggtactac
     4561 gctattaaat ctagacgcaa actacgttga atcagccaga accacaattg attattttgt
     4621 tgatttcata gataatgtat gtatggatga aatggtgcga gagtcgcaaa ggaatggaat
     4681 agcaccacaa tcagatgcct tgcggaagtt atcaggagtt aagttcagac gaataaactt
     4741 taacaattca tcagagtata ttgagaactg gaaccttcag aaccgcaggc aaaggacggg
     4801 attcacgttt cacaaaccga acatatttcc atattcggca tcattcacat tgaataggtc
     4861 gcagccgcag catgacaact taatgggcac aatgtggcta aacgctgggt cagaaataca
     4921 ggttgcagga tttgactatt catgcgcaat taacgctcca gctaacattc aacaatttga
     4981 acatatagtg caattacgga gggtattgac tactgcaacc attacgctac ttccggatgc
     5041 agagaggttt agtttcccaa gagttattaa ctcagcagac ggagcaacta cgtggtattt
     5101 caacccagta atattgcgtc caaataatgt agaagttgaa ttcttattga atggtcagat
     5161 catcaatact taccaagcta gattcggtac catcgtcgca agaaattttg ataccatacg
     5221 cctttctttt cagctgatga gaccaccaaa catgacacca gcagtggctg cacttttccc
     5281 gaatgctcaa ccgttcgagc atcatgcaac agtcggactg acgcttagga ttgactcagc
     5341 aatttgtgaa tcagttcttg cagacgcaag tgaaactatg ttggctaatg tgacgtcagt
     5401 cagacaagaa tacgcaatac cagtaggacc agtatttcca ccaggaatga attggaccga
     5461 cctcattact aattattcac catcaaggga ggacaatctg caacgtgtgt ttacagtagc
     5521 ttccattaga agcatgttgg taaagtgagc gaagcttatc gatgtcgacg tagttaacta
     5581 gcgtacgatc gactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     5641 ttcgttttat ctgttgtttg tccggccatc atggccgcgg tgatca
//
