p119L1e vector (V009785)

MSDS

Basic Vector Information

- - Vector Name:
  - p119L1e
- - Antibiotic Resistance:
  - Kanamycin
- - Length:
  - 8290 bp
- - Type:
  - Cloning vector
- - Replication origin:
  - ori
- - Source/Author:
  - Maclean JM, Williamson A-L., Jacobs WR.

p119L1e vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

p119L1e vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_2418        8290 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p119L1e, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Maclean JM, Williamson A-L., Jacobs WR.
  TITLE     Investigation of the use of recombinant BCG, expressing the major 
            capsid protein (L1) of human papillomavirus type 16, as a candidate 
            vaccine for cervical cancer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Maclean JM, Williamson A-L., Jacobs WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2005) Division of Medical Virology, Department of 
            Clinical Laboratory Science and Institute of Infectious Diseases and
            Molecular Medicine, University of Cape Town, Rondebosch, Cape Town 
            7701, South Africa
REFERENCE   3  (bases 1 to 8290)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8290)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-MAR-2005) Division of Medical Virology, Department of Clinical 
            Laboratory Science and Institute of Infectious Diseases and 
            Molecular Medicine, University of Cape Town, Rondebosch, Cape Town 
            7701, South Africa"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1877..2689
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5698..6330
                     /gene="tmk"
                     /label=tmk
                     /note="Thymidylate kinase from Mycobacterium tuberculosis 
                     (strain ATCC 25618 / H37Rv). Accession#: P9WKE1"
     CDS             6571..8085
                     /gene="L1"
                     /label=L1
                     /note="Major capsid protein L1 from Human papillomavirus
                     type 16. Accession#: P03101"
     terminator      8208..8254
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"

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