LOCUS       40924_2418        8290 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p119L1e, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Maclean JM, Williamson A-L., Jacobs WR.
  TITLE     Investigation of the use of recombinant BCG, expressing the major 
            capsid protein (L1) of human papillomavirus type 16, as a candidate 
            vaccine for cervical cancer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Maclean JM, Williamson A-L., Jacobs WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2005) Division of Medical Virology, Department of 
            Clinical Laboratory Science and Institute of Infectious Diseases and
            Molecular Medicine, University of Cape Town, Rondebosch, Cape Town 
            7701, South Africa
REFERENCE   3  (bases 1 to 8290)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8290)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-MAR-2005) Division of Medical Virology, Department of Clinical 
            Laboratory Science and Institute of Infectious Diseases and 
            Molecular Medicine, University of Cape Town, Rondebosch, Cape Town 
            7701, South Africa"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1877..2689
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5698..6330
                     /gene="tmk"
                     /label=tmk
                     /note="Thymidylate kinase from Mycobacterium tuberculosis 
                     (strain ATCC 25618 / H37Rv). Accession#: P9WKE1"
     CDS             6571..8085
                     /gene="L1"
                     /label=L1
                     /note="Major capsid protein L1 from Human papillomavirus
                     type 16. Accession#: P03101"
     terminator      8208..8254
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagaggtg accacaacga cgcgcccgct ttgatcgggg acgtctgcgg ccgaccattt
       61 acgggtcttg ttgtcgttgg cggtcatggg ccgaacatac tcacccggat cggagggccg
      121 aggacaaggt cgaacgaggg gcatgacccg gtgcggggct tcttgcactc ggcataggcg
      181 agtgctaaga ataacgttgg cactcgcgac cggtgagtcg taggtcggga cggtgaggcc
      241 aggcccgtcg tcgcagcgag tggcagcgag gacaacttga gccgtccgtc gcgggcactg
      301 cgcccggcca gcgtaagtag cggggttgcc gtcacccggt gacccccggt ttcatccccg
      361 atccggagga atcacttcgc aatggccaag acaattgcgg atccagatgt gaacgagctg
      421 ctgcacttag cgccgaatgt gtggccgcgc aatactactc gcgatgaagt cggtgtggtc
      481 tgcatcgcag gaattccact gacgcagctc gcccaggagt acgggacccc gctgttcgtc
      541 atcgacgagg acgactttcg ctcgcgctgc cgagaaaccg ccgcggcctt tggaagtggg
      601 gcgaacgtgc actatgccgc caaggcgttc ctgtgcagcg aagtagcccg gtggatcagc
      661 gaagaagggc tctgtctgga cgtttgcacc ggtggggagt tggcggtcgc gctgcacgct
      721 agctttccgc ccgagcgaat taccttgcac ggcaacaaca aatcggtctc agagttgacc
      781 gctgcggtca aagccggagt cggccatatt gtcgtcgatt cgatgaccga gatcgagcgc
      841 ctcgacgcca tcgcgggcga ggccggaatc gtccaggatg tcctggtgcg tctcaccgtc
      901 ggtgtcgagg cgcacaccca cgagttcatc tccaccgcgc acgaggacca gaaattcggg
      961 ttatcggtgg ccagcggcgc ggccatggca gcggtgcggc gcgttttcgc cactgatcac
     1021 ctgcgcctgg ttgggctaca cagccacatc ggttcgcaga tcttcgacgt ggacggcttc
     1081 gaactcgccg cgcaccgtgt catcggcctg ctacgcgacg tcgtcggcga gttcggtccc
     1141 gaaaagacgg cacagatcgc gaccgtcgat ctcggtggcg gcttgggcat ctcgtatttg
     1201 ccgtccgacg acccaccgcc gatagccgag ctcgcggcca agctgggtac catcgtgagc
     1261 gacgagtcaa cggccgtggg gctgccgacg cccaagctcg ttgtggagcc cggacgcgcc
     1321 atcgccggac cgggcaccat cacgttgtat gaggtcggca ccgttaagga cgtcgatgtc
     1381 agcgccacag cgcatcgacg ttacgtcagt gtcgacggcg gcatgagcga caacatccgc
     1441 accggcctct acggcgcgca gtatgacgtc cggctggtgt ctcgagtcag cgacgccccg
     1501 ccggtaccgg cccgtctggt cggaaagcac tgcgaaagtg gcgatatcat cgtgcgggac
     1561 acctgggtgc cgacgatatt cggcccggcg atctggttgc ggttgccgcc accggcctta
     1621 ctgctattcg ctgtcgagtc gttacaacat ggtcggccgt cccgctgtgg tagcggtgca
     1681 cgcggcaacg ctcgcctggt cctgcgtcgg gagacggtcg acgatttgct gagtttggaa
     1741 gtgaggtgaa ctagtctcta gccaacaaag cgacgttgtg tctcaaaatc tctgatgtta
     1801 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1861 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt
     1921 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1981 atcaggtgcg acaatctatc gcttgtatgg gaagccccat gcgccagagt tgtttctgaa
     2041 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2101 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2161 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga
     2221 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2281 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2341 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2401 tgaacaagtc tggaaagaaa tgcataatct tttgccattc tcaccggatt cagtcgtcac
     2461 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2521 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2581 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     2641 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     2701 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt
     2761 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca
     2821 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct
     2881 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat
     2941 gagtcagcaa caccttcttc acgaggcaga cctcactagt tccactgagc gtcagacccc
     3001 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3061 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     3421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc gtgagcccac
     3781 cagctccgta agttcgggtg ctgtgtggct cgtacccgcg cattcaggcg gcagggggtc
     3841 taacgggtct aaggcggcgt gtacggccgc cacagcggct cttagcggcc cggaaacgtc
     3901 ctcgaaacga cgcatgtgtt cctcctggtt ggtacaggtg gttgggggtg ctcggctgtc
     3961 gctggtgttt catcatcagg gctcgacggg agagcggggg agtgtgcagt tgtggggtgg
     4021 cccctcagcg aaatatctga cttggagctc gtgtcggacc atacaccggt gattaatcgt
     4081 ggtttattat caagcgtgag ccacgtcgcc gacgaatttg agcagctctg gctgccgtac
     4141 tggtccctgg caagcgacga tctgctcgag gggatctacc gccaaagccg cgcgtcggcc
     4201 ctaggccgcc ggtacatcga ggcgaaccca acagcgctgg caaacctgct ggtcgtggac
     4261 gtagaccatc cagacgcagc gctccgagcg ctcagcgccc gggggtccca tccgctgccc
     4321 aacgcgatcg tgggcaatcg cgccaacggc cacgcacacg cagtgtgggc actcaacgcc
     4381 cctgttccac gcaccgaata cgcgcggcgt aagccgctcg catacatggc ggcgtgcgcc
     4441 gaaggccttc ggcgcgccgt cgatggcgac cgcagttact caggcctcat gaccaaaaac
     4501 cccggccaca tcgcctggga aacggaatgg ctccactcag atctctacac actcagccac
     4561 atcgaggccg agctcggcgc gaacatgcca ccgccgcgct ggcgtcagca gaccacgtac
     4621 aaagcggctc cgacgccgct agggcggaat tgcgcactgt tcgattccgt caggttgtgg
     4681 gcctatcttc ccgccctcat gcggatctac ctgccgaccc ggaacgtgga cggactcggc
     4741 cgcgcgatct atgccgagtg ccacgcgcga aacgccgaat ttccgtgcaa cgacgtgtgt
     4801 cccggaccgc taccggacag cgaggtccgc gccatcgcca acagcatttg gcgttggatc
     4861 acaaccaagt cgcgcatttg ggcggacggg atcgtggtct acgaggccac actcagtgcg
     4921 cgccatgcgg ccatctcgcg gaagggcgca gcagcgcgca cggcggcgag cacagttgcg
     4981 cggcgcgcaa agtccgcgtc agccatggag gcattgctat gagcgacggc tacagcgacg
     5041 gctacagcga cggctacaac tggcagccga ctgtccgcaa aaagcggcgc gtgaccgccg
     5101 ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt cgtccggctc gtggcgcagg
     5161 aacgcagcga gtggttcgcc gagcaggctg cacgccgcga acgcatccgc gcctatcacg
     5221 acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat ctggacaccg
     5281 ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa gcggctcaaa
     5341 aggcccacaa cgaagccgac aatccaccgc tgttctaacg caattgggga gcgggtgtcg
     5401 cgggggttcc gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc ctggtagacg
     5461 ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgt ccgttttgaa
     5521 taccagccag acgagacggg gttctacgaa tcttggtcga taccaagcca tttccgctga
     5581 atatcgtgga gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc gaactccgtt
     5641 gtagtgcttg tggtggcatc cgtggcgcgg ccgcggtacc agatctttaa atctagagcg
     5701 attgagggcg ttgacggcgc tggcaagcgg acgttggtgg aaaagctgtc cggggccttt
     5761 cgagcagccg ggagatcggt ggccacactg gcgttcccgc gctacggaca gtcggtggcc
     5821 gccgacatcg cagcggaggc gctgcacggc gagcacggtg acctcgcatc gtcggtgtat
     5881 gcgatggcga cgctgttcgc gctcgaccgc gctggcgcgg tccacacgat ccaggggctg
     5941 tgtcgcggct acgacgtggt gatcctggat cgctacgtcg cctccaacgc ggcctacagc
     6001 gcggcgcgcc tacatgaaaa cgcggccggg aaggcagcgg cctgggttca gcggatcgaa
     6061 tttgcaagac tcgggttgcc caagcccgac tggcaggtgc tccttgcggt ctctgccgag
     6121 ctcgccgggg aacgatcccg cggccgtgcc cagcgtgacc ccggtcgggc gcgcgacaat
     6181 tacgaacgcg acgctgaact tcagcagcgc accggtgcgg tctacgccga gttggcggcc
     6241 caagggtggg gcggccggtg gctggttgtc ggcgccgatg ttgatccggg ccgactagcg
     6301 gcgactttgg cgcctccaga cgtgccaagt tgagggtatt tgccctaatc aagcaagaaa
     6361 cgcccgtgtg gtgcgccagt tttgtcccga tgtggtgaca ccatggacca tatgaagcgt
     6421 ggactgacgg tcgcggtagc cggagccgcc attctggtcg caggtctttc cggatgttca
     6481 agcaacaagt cgactacagg aagcggtgag accacgaccg cggcaggcac gacggcaagc
     6541 cccggcggtg ggcccacagc tggtcgacat atgtctcttt ggctgcctag tgaggccact
     6601 gtctacttgc ctcctgtccc agtatctaag gttgtaagca cggatgaata tgttgcacgc
     6661 acaaacatat attatcatgc agggacatcc agactacttg cagttggaca tccctatttt
     6721 cctattaaaa aacctaacaa taacaaaata ttagttccta aagtatcagg attacaatac
     6781 agggtattta gaatacattt acctgacccc aataagtttg gttttcctga cacctcattt
     6841 tataatccag atacacagcg gctggtttgg gcctgtgtag gtgttgaggt aggccgtggt
     6901 cagccattag gtgtgggcat tagtggccat cctttattaa ataaattgga tgacacagaa
     6961 aatgctagtg cttatgcagc aaatgcaggt gtggataata gagaatgtat atctatggat
     7021 tacaaacaaa cacaattgtg tttaattggt tgcaaaccac ctatagggga acactggggc
     7081 aaaggatccc catgtaccaa tgttgcagta aatccaggtg attgtccacc attagagtta
     7141 ataaacacag ttattcagga tggtgatatg gttgatactg gctttggtgc tatggacttt
     7201 actacattac aggctaacaa aagtgaagtt ccactggata tttgtacatc tatttgcaaa
     7261 tatccagatt atattaaaat ggtgtcagaa ccatatggcg acagcttatt tttttattta
     7321 cggagggaac aaatgtttgt tagacattta tttaataggg ctggtactgt tggtgaaaat
     7381 gtaccagacg atttatacat taaaggctct gggtctactg caaatttagc cagttcaaat
     7441 tattttccta cacctagtgg ttctatggtt acctctgatg cccaaatatt caataaacct
     7501 tattggttac aacgagcaca gggccacaat aatggcattt gttggggtaa ccaactattt
     7561 gttactgttg ttgatactac acgcagtaca aatatgtcat tatgtgctgc catatctact
     7621 tcagaaacta catataaaaa tactaacttt aaggagtacc tacgacatgg ggaggaatat
     7681 gatttacagt ttatttttca actgtgcaaa ataaccttaa ctgcagacgt tatgacatac
     7741 atacattcta tgaattccac tattttggag gactggaatt ttggtctaca acctccccca
     7801 ggaggcacac tagaagatac ttataggttt gtaacatccc aggcaattgc ttgtcaaaaa
     7861 catacacctc cagcacctaa agaagatccc cttaaaaaat acactttttg ggaagtaaat
     7921 ttaaaggaaa agttttctgc agacctagat cagtttcctt taggacgcaa atttttacta
     7981 caagcaggat tgaaggccaa accaaaattt acattaggaa aacgaaaagc tacacccacc
     8041 acctcatcta cctctacaac tgctaaacgc aaaaaacgta agctgtatcg attgcgcggc
     8101 ccgggccgcg ccttcgtcac catcacgttt aaacagatcc cgaacccgct gctgggcctg
     8161 gatcgatgtc gacgtagtta actagcgtac gatcgactgc caggcatcaa ataaaacgaa
     8221 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtccggc catcatggcc
     8281 gcggtgatca
//
