Basic Vector Information
- Vector Name:
- p1168hIL6mNFkB-luc+
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5631 bp
- Type:
- Reporter vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
p1168hIL6mNFkB-luc+ vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
p1168hIL6mNFkB-luc+ vector Sequence
LOCUS 40924_2408 5631 bp DNA circular SYN 18-DEC-2018 DEFINITION Reporter vector p1168hIL6mNFkB-luc+, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5631) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 5631) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 5631) TITLE Direct Submission REFERENCE 4 (bases 1 to 5631) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5631 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 32..672 /label=5' FR of hIL6 /note="5' FR of hIL6" regulatory 673..1208 /label=hIL6 promoter mutant /note="hIL6 promoter mutant" /regulatory_class="promoter" CDS 1273..2922 /label=luciferase /note="firefly luciferase" polyA_signal complement(2966..3087) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(3506..4094) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4268..5125) /label=AmpR /note="beta-lactamase" promoter complement(5126..5230) /label=AmpR promoter polyA_signal 5257..5391 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" regulatory complement(5472..5568) /label=SV40 polyA late /note="SV40 polyA late" /regulatory_class="terminator" regulatory 5472..5568 /label=SV40 polyA early /note="SV40 polyA early" /regulatory_class="terminator" polyA_signal 5494..5628 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal"
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