LOCUS       40924_2408        5631 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector p1168hIL6mNFkB-luc+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5631)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5631)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    32..672
                     /label=5' FR of hIL6
                     /note="5' FR of hIL6"
     regulatory      673..1208
                     /label=hIL6 promoter mutant
                     /note="hIL6 promoter mutant"
                     /regulatory_class="promoter"
     CDS             1273..2922
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2966..3087)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3506..4094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4268..5125)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5126..5230)
                     /label=AmpR promoter
     polyA_signal    5257..5391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(5472..5568)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      5472..5568
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     polyA_signal    5494..5628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcctcct gcaagagaca ccatcctgag
       61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag
      121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt
      181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg
      241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac
      301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag
      361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac
      421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg
      481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc
      541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg
      601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag
      661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga
      721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg
      781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa
      841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac
      901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg
      961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc
     1021 tagttgtgtc ttgcgatgct aaaggacgtc acattgcaca atcttaataa ggtttccaat
     1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgagat
     1141 cttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc
     1201 tgaggctcat tctgccctcg agatctgcga tctaagtaag cttggcattc cggtactgtt
     1261 ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg
     1321 ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
     1381 cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac
     1441 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
     1501 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
     1561 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
     1621 agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt
     1681 ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg
     1741 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
     1801 aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg
     1861 aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc
     1921 gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
     1981 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
     2041 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc
     2101 cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc
     2161 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc
     2221 gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc
     2281 aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
     2341 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
     2401 gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg
     2461 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
     2521 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac
     2581 cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc
     2641 atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac
     2701 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
     2761 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
     2821 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
     2881 gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaattcta gagtcggggc
     2941 ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     3001 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3061 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3121 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     3181 atcgataagg atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     3241 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     3301 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3481 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4201 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4261 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4321 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4381 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4441 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4501 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4561 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4621 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4681 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4741 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4801 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4861 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4921 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4981 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5041 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5101 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5161 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5221 gggttccgcg cacatttccc cgaaaagtgc cacctgaact tgtttattgc agcttataat
     5281 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5341 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat cataatcagc
     5401 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     5461 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     5521 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5581 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctc g
//
