Basic Vector Information
p(GRE)2-50huIL6P-luc+ vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
p(GRE)2-50huIL6P-luc+ vector Sequence
LOCUS 40924_2308 4950 bp DNA circular SYN 18-DEC-2018 DEFINITION Reporter vector p(GRE)2-50huIL6P-luc+, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4950) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 4950) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 4950) TITLE Direct Submission REFERENCE 4 (bases 1 to 4950) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4950 /mol_type="other DNA" /organism="synthetic DNA construct" regulatory 39..98 /label=GRE2 inducer /note="GRE2 inducer" /regulatory_class="promoter" regulatory 119..168 /label=hIL6 promoter fragment /note="hIL6 promoter fragment" /regulatory_class="promoter" CDS 216..1865 /label=luciferase /note="firefly luciferase" polyA_signal complement(1909..2030) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(2453..3041) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3215..4072) /label=AmpR /note="beta-lactamase" promoter complement(4073..4177) /label=AmpR promoter rep_origin 4204..4659 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" polyA_signal 4790..4838 /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 4852..4943 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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