LOCUS       40924_2308        4950 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector p(GRE)2-50huIL6P-luc+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4950)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4950)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      39..98
                     /label=GRE2 inducer
                     /note="GRE2 inducer"
                     /regulatory_class="promoter"
     regulatory      119..168
                     /label=hIL6 promoter fragment
                     /note="hIL6 promoter fragment"
                     /regulatory_class="promoter"
     CDS             216..1865
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1909..2030)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2453..3041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3215..4072)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4073..4177)
                     /label=AmpR promoter
     rep_origin      4204..4659
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4790..4838
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4852..4943
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tctctgctgt acaggatgtt
       61 ctagcggatc ctgctgtaca ggatgttcta gctacctgca ggtcgactct agaggatcat
      121 tagagtctca acccccaata aatataggac tggagatgtc tgaggctcat tctgccctcg
      181 aagcttggca ttccggtact gttggtaaag ccaccatgga agacgccaaa aacataaaga
      241 aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg
      301 ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg
      361 tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac
      421 gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct
      481 ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt
      541 ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt
      601 ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa
      661 ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca
      721 catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg
      781 acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg
      841 ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca
      901 atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa
      961 tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg
     1021 aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc
     1081 caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt
     1141 tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca
     1201 agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta
     1261 ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt
     1321 ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg
     1381 aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca
     1441 acgccttgat tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag
     1501 acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg
     1561 tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg
     1621 tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc
     1681 acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg
     1741 cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa
     1801 aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg
     1861 ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg
     1921 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     1981 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     2041 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     2101 aaaacctcta caaatgtggt aaaatcgata aggatccgtc gatcgaccga tgcccttgag
     2161 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     2221 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt
     2281 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2341 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2401 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2461 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2521 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2581 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2641 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2701 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2761 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2821 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2881 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2941 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3001 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3061 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3121 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3181 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3241 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3301 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3361 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3421 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3481 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3541 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3601 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3661 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3721 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3781 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3841 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3901 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3961 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4021 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4081 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4141 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4201 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4261 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4321 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4381 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4441 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4501 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4561 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4621 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccattcgcca ttcaggctgc
     4681 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac
     4741 catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc
     4801 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
     4861 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
     4921 tgcaggtgcc agaacatttc tctatcgata
//
