OX4570 vector (V009817)

Basic Vector Information

      • Vector Name:
      • OX4570
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6520 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Martins S, Naish N, Walker AS, Morrison NI, Scaife S, Fu G, Dafa'alla T, Alphey L.

OX4570 vector Vector Map

OX45706520 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300Opie2nuclear localization signalDsRed23'UTRM13 fwdAmpR promoterAmpRoriCAP binding sitelac promoterlac operatorM13 rev

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

OX4570 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6520 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector OX4570, complete sequence.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Martins S, Naish N, Walker AS, Morrison NI, Scaife S, Fu G, 
            Dafa'alla T, Alphey L.
  TITLE     Germline transformation of the diamondback moth, Plutella xylostella
            L., using the piggyBac transposable element
  JOURNAL   Insect Mol. Biol. 21 (4), 414-421 (2012)
  PUBMED    22621377
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   Martins S, Naish N, Walker A, Morrison N, Scaife S, Fu G, Dafa'alla 
            T, Alphey L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2012) Research, Oxitec Ltd, 71 Milton Park, 
            Abingdon, Oxon OX14 4RX, UK
REFERENCE   3  (bases 1 to 6520)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Cloning vector OX4570"
                     /mol_type="other DNA"
     mobile_element  1..675
                     /mobile_element_type="transposon:piggyBac 5'"
     regulatory      733..965
     misc_feature    967..993
                     /label=nuclear localization signal
                     /note="nuclear localization signal"
     CDS             1002..1679
                     /note="fluorescent protein, variant of DsRed, from 
                     Discosoma sp."
     3'UTR           1681..1908
     polyA_signal    1787..1908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  1969..3017
                     /mobile_element_type="transposon:piggyBac 3'"
     primer_bind     complement(3718..3734)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
     promoter        4208..4312
                     /label=AmpR promoter
     CDS             4313..5173
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      5344..5932
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     protein_bind    6220..6241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6256..6286
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6294..6310
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6318..6334
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 

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