LOCUS       40924_2298        6520 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector OX4570, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Martins S, Naish N, Walker AS, Morrison NI, Scaife S, Fu G, 
            Dafa'alla T, Alphey L.
  TITLE     Germline transformation of the diamondback moth, Plutella xylostella
            L., using the piggyBac transposable element
  JOURNAL   Insect Mol. Biol. 21 (4), 414-421 (2012)
  PUBMED    22621377
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   Martins S, Naish N, Walker A, Morrison N, Scaife S, Fu G, Dafa'alla 
            T, Alphey L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2012) Research, Oxitec Ltd, 71 Milton Park, 
            Abingdon, Oxon OX14 4RX, UK
REFERENCE   3  (bases 1 to 6520)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6520)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Insect Mol.
            Biol."; date: "2012"; volume: "21"; issue: "4"; pages: "414-421"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JAN-2012) Research, Oxitec Ltd, 71 Milton Park, Abingdon, Oxon 
            OX14 4RX, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..675
                     /mobile_element_type="transposon:piggyBac 5'"
                     /label=5'
     regulatory      733..965
                     /label=Opie2
                     /note="Opie2"
                     /regulatory_class="promoter"
     misc_feature    967..993
                     /label=nuclear localization signal
                     /note="nuclear localization signal"
     CDS             1002..1676
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     polyA_signal    1787..1908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  1969..3017
                     /mobile_element_type="transposon:piggyBac 3'"
                     /label=3'
     primer_bind     complement(3718..3734)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4208..4312
                     /label=AmpR promoter
     CDS             4313..5170
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5344..5932
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6220..6241
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6256..6286
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6294..6310
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6318..6334
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaccctagaa agatagtctg cgtaaaattg acgcatgcat tcttgaaata ttgctctctc
       61 tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc
      121 tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga ttttgaacta taacgaccgc
      181 gtgagtcaaa atgacgcatg attatctttt acgtgacttt taagatttaa ctcatacgat
      241 aattatattg ttatttcatg ttctacttac gtgataactt attatatata tattttcttg
      301 ttatagatat cgtgactaat atataataaa atgggtagtt ctttagacga tgagcatatc
      361 ctctctgctc ttctgcaaag cgatgacgag cttgttggtg aggattctga cagtgaaata
      421 tcagatcacg taagtgaaga tgacgtccag agcgatacag aagaagcgtt tatagatgag
      481 gtacatgaag tgcagccaac gtcaagcggt agtgaaatat tagacgaaca aaatgttatt
      541 gaacaaccag gttcttcatt ggcttctaac agaatcttga ccttgccaca gaggactatt
      601 agaggtaaga ataaacattg ttggtcaact tcaaagtcca cgaggcgtag ccgagtctct
      661 gcactgaaca ttgtcagatc ggcccggtat cctaggattt aattaacgag gttcccacaa
      721 tggttaattc gagctcgccc ggggatctaa ttcaattaga gactaattca attagagcta
      781 attcaattag gatccaagct tatcgatttc gaaccctcga ccgccggagt ataaatagag
      841 gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc
      901 gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatcgggg taccgctaga
      961 gtcgatccca ccccacccaa gaagaagcgc aaaccggtac catggcctcc tccgagaacg
     1021 tcatcaccga gttcatgcgc ttcaaggtgc gcatggaggg caccgtgaac ggccacgagt
     1081 tcgagatcga gggcgagggc gagggccgcc cctacgaggg ccacaacacc gtgaagctga
     1141 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt
     1201 acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag aagctgtcct
     1261 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggcgaccg
     1321 tgacccagga ctcctccctg caggacggct gcttcatcta caaggtgaag ttcatcggcg
     1381 tgaacttccc ctccgacggc cccgtgatgc agaagaagac catgggctgg gaggcctcca
     1441 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gacccacaag gccctgaagc
     1501 tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc aagaagcccg
     1561 tgcagctgcc cggctactac tacgtggacg ccaagctgga catcacctcc cacaacgagg
     1621 actacaccat cgtggagcag tacgagcgca ccgagggccg ccaccacctg ttcctgtgat
     1681 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     1741 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     1801 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1861 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac gggtaccata
     1921 ctagtgtgca tgcgtgcggc cgctcattta aatctggccg gccgcaacca ttgtgggaac
     1981 cgtgcgatca aacaaacgcg agataccgga agtactgaaa aacagtcgct ccaggccagt
     2041 gggaacatcg atgttttgtt ttgacggacc ccttactctc gtctcatata aaccgaagcc
     2101 agctaagatg gtatacttat tatcatcttg tgatgaggat gcttctatca acgaaagtac
     2161 cggtaaaccg caaatggtta tgtattataa tcaaactaaa ggcggagtgg acacgctaga
     2221 ccaaatgtgt tctgtgatga cctgcagtag gaagacgaat aggtggccta tggcattatt
     2281 gtacggaatg ataaacattg cctgcataaa ttcttttatt atatacagcc ataatgtcag
     2341 tagcaaggga gaaaaggtcc aaagtcgcaa aaaatttatg agaaaccttt acatgagcct
     2401 gacgtcatcg tttatgcgta agcgtttaga agctcctact ttgaagagat atttgcgcga
     2461 taatatctct aatattttgc caaatgaagt gcctggtaca tcagatgaca gtactgaaga
     2521 gccagtaatg aaaaaacgta cttactgtac ttactgcccc tctaaaataa ggcgaaaggc
     2581 aaatgcatcg tgcaaaaaat gcaaaaaagt tatttgtcga gagcataata ttgatatgtg
     2641 ccaaagttgt ttctgactga ctaataagta taatttgttt ctattatgta taagttaagc
     2701 taattactta ttttataata caacatgact gtttttaaag tacaaaataa gtttattttt
     2761 gtaaaagaga gaatgtttaa aagttttgtt actttataga agaaattttg agtttttgtt
     2821 tttttttaat aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta
     2881 agtgtaaata taataaaact taatatctat tcaaattaat aaataaacct cgatatacag
     2941 accgataaaa cacatgcgtc aattttacgc atgattatct ttaacgtacg tcacaatatg
     3001 attatctttc tagggttaaa taatagtttc taattttttt attattcagc ctgctgtcgt
     3061 gaataccgta tatctcaacg ctgtctgtga gattgtcgta ttctagcctt tttagttttt
     3121 cgctcatcga cttgatattg tccgacacat tttcgtcgat ttgcgttttg atcaaagact
     3181 tgagcagaga cacgttaatc aactgttcaa attgatccat attaacgata tcaacccgat
     3241 gcgtatatgg tgcgtaaaat atatttttta accctcttat actttgcact ctgcgttaat
     3301 acgcgttcgt gtacagacgt aatcatgttt tcttttttgg ataaaactcc tactgagttt
     3361 gacctcatat tagaccctca caagttgcaa aacgtggcat tttttaccaa tgaagaattt
     3421 aaagttattt taaaaaattt catcacagat ttaaagaaga accaaaaatt aaattatttc
     3481 aacagtttaa tcgaccagtt aatcaacgtg tacacagacg cgtcggcaaa aaacacgcag
     3541 cccgacgtgt tggctaaaat tattaaatca acttgtgtta tagtcacgga tttgccgtcc
     3601 aacgtgttcc tcaaaaagtt gaagaccaac aagtttacgg acactattaa ttatttgatt
     3661 ttgccccact tcattttgtg ggatcacaat tttgttatat tttaaacaaa gcttggcact
     3721 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3781 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3841 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     3901 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     3961 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4021 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4081 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4141 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4201 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4261 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4321 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4381 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4441 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4501 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4561 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4621 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4681 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4741 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4801 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4861 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4921 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4981 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5041 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5101 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5161 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5221 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5281 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5341 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5401 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5461 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5521 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5581 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5641 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5701 gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
     5761 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5821 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5881 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5941 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6001 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6061 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6121 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     6181 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     6241 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     6301 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaatttc gacctgcagg
     6361 catgcaagct tgcatgcctg caggtcgacg ctcgcgcgac ttggtttgcc attctttagc
     6421 gcgcgtcgcg tcacacagct tggccacaat gtggtttttg tcaaacgaag attctatgac
     6481 gtgtttaaag tttaggtcga gtaaagcgca aatctttttt
//
