Basic Vector Information
- Vector Name:
- OX4389
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7990 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Martins S, Naish N, Walker AS, Morrison NI, Scaife S, Fu G, Dafa'alla T, Alphey L.
OX4389 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
OX4389 vector Sequence
LOCUS 40924_2293 7990 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector OX4389, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7990)
AUTHORS Martins S, Naish N, Walker AS, Morrison NI, Scaife S, Fu G,
Dafa'alla T, Alphey L.
TITLE Germline transformation of the diamondback moth, Plutella xylostella
L., using the piggyBac transposable element
JOURNAL Insect Mol. Biol. 21 (4), 414-421 (2012)
PUBMED 22621377
REFERENCE 2 (bases 1 to 7990)
AUTHORS Martins S, Naish N, Walker A, Morrison N, Scaife S, Fu G, Dafa'alla
T, Luke A.
TITLE Direct Submission
JOURNAL Submitted (03-JAN-2012) Research, Oxitec Ltd, 71 Milton Park,
Abingdon, Oxon OX14 4RX, UK
REFERENCE 3 (bases 1 to 7990)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7990)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Insect Mol.
Biol."; date: "2012"; volume: "21"; issue: "4"; pages: "414-421"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(03-JAN-2012) Research, Oxitec Ltd, 71 Milton Park, Abingdon, Oxon
OX14 4RX, UK"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7990
/mol_type="other DNA"
/organism="synthetic DNA construct"
mobile_element 1..1426
/mobile_element_type="transposon:piggyBac 5'"
/label=5'
polyA_signal complement(1467..1588)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature complement(1704..1768)
/label=MCS
/note="multiple cloning site"
CDS complement(1769..2461)
/label=ZsGreen1
/note="Zoanthus green fluorescent protein"
misc_feature complement(2495..2521)
/label=nuclear localization signal
/note="nuclear localization signal"
regulatory complement(2525..3065)
/label=Opie2
/note="Opie2"
/regulatory_class="promoter"
protein_bind 3544..3643
/label=phage phi-C31 attP
/note="attachment site of phage phi-C31"
mobile_element 3718..4494
/mobile_element_type="transposon:piggyBac 3'"
/label=3'
primer_bind complement(5195..5211)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 5685..5789
/label=AmpR promoter
CDS 5790..6647
/label=AmpR
/note="beta-lactamase"
rep_origin 6821..7409
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 7697..7718
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7733..7763
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7771..7787
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7795..7811
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
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