LOCUS       40924_2293        7990 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector OX4389, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Martins S, Naish N, Walker AS, Morrison NI, Scaife S, Fu G, 
            Dafa'alla T, Alphey L.
  TITLE     Germline transformation of the diamondback moth, Plutella xylostella
            L., using the piggyBac transposable element
  JOURNAL   Insect Mol. Biol. 21 (4), 414-421 (2012)
  PUBMED    22621377
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Martins S, Naish N, Walker A, Morrison N, Scaife S, Fu G, Dafa'alla 
            T, Luke A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2012) Research, Oxitec Ltd, 71 Milton Park, 
            Abingdon, Oxon OX14 4RX, UK
REFERENCE   3  (bases 1 to 7990)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7990)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Insect Mol.
            Biol."; date: "2012"; volume: "21"; issue: "4"; pages: "414-421"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JAN-2012) Research, Oxitec Ltd, 71 Milton Park, Abingdon, Oxon 
            OX14 4RX, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..1426
                     /mobile_element_type="transposon:piggyBac 5'"
                     /label=5'
     polyA_signal    complement(1467..1588)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(1704..1768)
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(1769..2461)
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     misc_feature    complement(2495..2521)
                     /label=nuclear localization signal
                     /note="nuclear localization signal"
     regulatory      complement(2525..3065)
                     /label=Opie2
                     /note="Opie2"
                     /regulatory_class="promoter"
     protein_bind    3544..3643
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     mobile_element  3718..4494
                     /mobile_element_type="transposon:piggyBac 3'"
                     /label=3'
     primer_bind     complement(5195..5211)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5685..5789
                     /label=AmpR promoter
     CDS             5790..6647
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6821..7409
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7697..7718
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7733..7763
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7771..7787
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7795..7811
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaccctagaa agatagtctg cgtaaaattg acgcatgcat tcttgaaata ttgctctctc
       61 tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc
      121 tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga ttttgaacta taacgaccgc
      181 gtgagtcaaa atgacgcatg attatctttt acgtgacttt taagatttaa ctcatacgat
      241 aattatattg ttatttcatg ttctacttac gtgataactt attatatata tattttcttg
      301 ttatagatat cgtgactaat atataataaa atgggtagtt ctttagacga tgagcatatc
      361 ctctctgctc ttctgcaaag cgatgacgag cttgttggtg aggattctga cagtgaaata
      421 tcagatcacg taagtgaaga tgacgtccag agcgatacag aagaagcgtt tatagatgag
      481 gtacatgaag tgcagccaac gtcaagcggt agtgaaatat tagacgaaca aaatgttatt
      541 gaacaaccag gttcttcatt ggcttctaac agaatcttga ccttgccaca gaggactatt
      601 agaggtaaga ataaacattg ttggtcaact tcaaagtcca cgaggcgtag ccgagtctct
      661 gcactgaaca ttgtcagatc tcaaagaggt ccgacgcgta tgtgccgcaa tatatatgac
      721 ccacttttat gcttcaaact attttttact gatgagataa tttcggaaat tgtaaaatgg
      781 acaaatgctg agatatcatt gaaacgtcgg gaatctatga caggtgctac atttcgtgac
      841 acgaatgaag atgaaatcta tgctttcttt ggtattctgg taatgacagc agtgagaaaa
      901 gataaccaca tgtccacaga tgacctcttt gatcgatctt tgtcaatggt gtacgtctct
      961 gtaatgagtc gtgatcgttt tgattttttg atacgatgtc ttagaatgga tgacaaaagt
     1021 atacggccca cacttcgaga aaacgatgta tttactcctg ttagaaaaat atgggatctc
     1081 tttatccatc agtgcataca aaattacact ccaggggctc atttgaccat agatgaacag
     1141 ttacttggtt ttagaggacg gtgtccgttt aggatgtata tcccaaacaa gccaagtaag
     1201 tatggaataa aaatcctcat gatgtgtgac agtggtacga agtatatgat aaatggaatg
     1261 ccttatttgg gaagaggaac acagaccaac ggagtaccac tcggtgaata ctacgtgaag
     1321 gagttatcaa agcctgtgca cggtagttgt cgtaatatta cgtgtgacaa ttggttcacc
     1381 tcaatccctt tggcaaaaaa cttactacaa gaaccgtata agttcgagat cggccggcct
     1441 aggcgcgcct cttaattaac tcgcgttaag atacattgat gagtttggac aaaccacaac
     1501 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     1561 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     1621 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     1681 ggctgattat gatcagttat ctagatcccg ggcccgcggt accgtcgact gcagaattcg
     1741 aagcttgagc tcgagatctg agtccggagg gcaaggcgga gccggaggcg atggcgtgct
     1801 cggtcaggtg ccacttctgg ttcttggcgt cgctgcggtc ctcgcgggtc agcttgtgct
     1861 ggatgaagtg ccagtcgggc atcttgcggg gcacggactt ggccttgtac acggtgtcga
     1921 actggcagcg caagcggcca ccgtccttca gcagcaggta catgctcacg tcgcccttca
     1981 agatgccctg cttgggcacg gggatgatct tctcgcagga gggctcccag ttgtcggtca
     2041 tcttcttcat cacggggccg tcggcgggga agttcacgcc gtagaacttg gactcgtggt
     2101 acatgcagtt ctcctccacg ctcacggtga tgtcggcgtt gcagatgcac acggcgccgt
     2161 cctcgaacag gaaggagcgg tcccaggtgt agccggcggg gcaggagttc ttgaagtagt
     2221 cgacgatgtc ctgggggtac tcggtgaaca cgcggttgcc gtacatgaag gcggcggaca
     2281 agatgtcctc ggcgaagggc aaggggccgc cctccaccac gcacaggttg atggcctgct
     2341 tgcccttgaa ggggtagccg atgccctcgc cggtgatcac gaacttgtgg ccgtccacgc
     2401 agccctccat gcggtacttc atggtcatct ccttggtcag gccgtgcttg gactgggcca
     2461 tggtggcgac cggtagcgct agcgcaggta ccggtttgcg cttcttcttg ggtggggtgg
     2521 gatccaccag agacaggttg cggcggcggt tggatggcgt gggcgcgttg gcgttgttgg
     2581 accggctcat gttgtgtcgc tgtaacagat gctgttcaac tgtgtttacc agatcgttgc
     2641 gggctgtatt tataggcgcg ataagcggga cgggcgcctc gtgtccggtc acgcgcatga
     2701 gataacgcgc ggctgatatg gaggcgcgtc ctgttccgat aaggagttgc gtccggctgc
     2761 ggttagcaac acaggaagct ggcgtcctgt cacgataaga caacactcgt ccggtccgat
     2821 aatgtgattc gtacgtgaca ggacgcgacc cgataaggcc ggcctacgtg actgccgaca
     2881 cgtacttttt tgcactgcaa aaaggttcaa tgtgtggtag tgtatttgga gcgtatacaa
     2941 cggtgtagac tatttatgta aaatagtcta cgaaacgtag agtttgtact atgtatgggc
     3001 ccgcgtgcaa aagcgtgttt ttttgcagtg caaaaaagtt ggtggtgggg aggccaccga
     3061 gtatggtacc atgcggccgc tcatttaaat atttcgaaat cgataagctt ggatcctaat
     3121 tgaattagct ctaattgaat tagtctctaa ttgaattaga tccccgggcg agctcgaatt
     3181 aaccattgtg ggaaccgtgc gatcaaacaa acgcgagata ccggaagtac tgaaaaacag
     3241 tcgctccagg ccagtgggaa catcgatgtt ttgttttgac ggacccctta ctctcgtctc
     3301 atataaaccg aagccagcta agatggtata cttattatca tcttgtgatg aggatgcttc
     3361 tatcaacgaa agtaccggta aaccgcaaat ggttatgtat tataatcaaa ctaaaggcgg
     3421 agtggacacg ctagaccaaa tgtgttctgt gatgacctgc agcccggggg atccactagt
     3481 actgacggac acaccgaagc cccggcggca accctcagcg gatgccccgg ggcttcacgt
     3541 tttcccaggt cagaagcggt tttcgggagt agtgccccaa ctggggtaac ctttgagttc
     3601 tctcagttgg gggcgtaggg ccgccgacat gacacaaggg gttgtgaccg gggtggacac
     3661 gtacgcgggt gcttacgacc gtcagtcgcg cgagcgcgac tagttctaga gcggccgcct
     3721 gcagtaggaa gacgaatagg tggcctatgg cattattgta cggaatgata aacattgcct
     3781 gcataaattc ttttattata tacagccata atgtcagtag caagggagaa aaggtccaaa
     3841 gtcgcaaaaa atttatgaga aacctttaca tgagcctgac gtcatcgttt atgcgtaagc
     3901 gtttagaagc tcctactttg aagagatatt tgcgcgataa tatctctaat attttgccaa
     3961 atgaagtgcc tggtacatca gatgacagta ctgaagagcc agtaatgaaa aaacgtactt
     4021 actgtactta ctgcccctct aaaataaggc gaaaggcaaa tgcatcgtgc aaaaaatgca
     4081 aaaaagttat ttgtcgagag cataatattg atatgtgcca aagttgtttc tgactgacta
     4141 ataagtataa tttgtttcta ttatgtataa gttaagctaa ttacttattt tataatacaa
     4201 catgactgtt tttaaagtac aaaataagtt tatttttgta aaagagagaa tgtttaaaag
     4261 ttttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca
     4321 taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa
     4381 tatctattca aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat
     4441 tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag ggttaaataa
     4501 tagtttctaa tttttttatt attcagcctg ctgtcgtgaa taccgtatat ctcaacgctg
     4561 tctgtgagat tgtcgtattc tagccttttt agtttttcgc tcatcgactt gatattgtcc
     4621 gacacatttt cgtcgatttg cgttttgatc aaagacttga gcagagacac gttaatcaac
     4681 tgttcaaatt gatccatatt aacgatatca acccgatgcg tatatggtgc gtaaaatata
     4741 ttttttaacc ctcttatact ttgcactctg cgttaatacg cgttcgtgta cagacgtaat
     4801 catgttttct tttttggata aaactcctac tgagtttgac ctcatattag accctcacaa
     4861 gttgcaaaac gtggcatttt ttaccaatga agaatttaaa gttattttaa aaaatttcat
     4921 cacagattta aagaagaacc aaaaattaaa ttatttcaac agtttaatcg accagttaat
     4981 caacgtgtac acagacgcgt cggcaaaaaa cacgcagccc gacgtgttgg ctaaaattat
     5041 taaatcaact tgtgttatag tcacggattt gccgtccaac gtgttcctca aaaagttgaa
     5101 gaccaacaag tttacggaca ctattaatta tttgattttg ccccacttca ttttgtggga
     5161 tcacaatttt gttatatttt aaacaaagct tggcactggc cgtcgtttta caacgtcgtg
     5221 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5281 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5341 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     5401 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5461 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5521 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5581 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5641 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5701 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5761 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5821 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5881 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5941 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6001 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6061 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6121 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6181 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6241 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6301 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6361 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6421 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6481 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6541 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6601 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6661 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6721 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6781 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6841 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6901 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6961 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7021 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7081 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7141 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7201 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7261 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7321 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7381 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7441 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7501 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7561 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7621 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7681 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7741 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7801 acagctatga ccatgattac gaattcgagc tcggtacccg gggatcctct agagtcgacg
     7861 ctcgcgcgac ttggtttgcc attctttagc gcgcgtcgcg tcacacagct tggccacaat
     7921 gtggtttttg tcaaacgaag attctatgac gtgtttaaag tttaggtcga gtaaagcgca
     7981 aatctttttt
//
