NX33_GW vector (V009826)

LOCUS       40924_2274        7405 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector NX33_GW, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Lalonde S, Sero A, Pratelli R, Pilot G, Chen J, Sardi MI, Parsa SA, 
            Kim DY, Acharya BR, Stein EV, Hu HC, Villiers F, Takeda K, Yang Y, 
            Han YS, Schwacke R, Chiang W, Kato N, Loque D, Assmann SM, Kwak JM, 
            Schroeder JI, Rhee SY, Frommer WB.
  TITLE     A Membrane Protein/Signaling Protein Interaction Network for 
            Arabidopsis Version AMPv2
  JOURNAL   Front Physiol 1, 24 (2010)
  PUBMED    21423366
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   Lalonde S, Frommer WB.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2010) Plant Biology, Carnegie Institution, 260 
            Panama St, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 7405)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7405)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Physiol 1, 24 (2010)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2010) Plant Biology, Carnegie Institution, 260 Panama St, 
            Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      245..606
                     /label=TRP1
                     /note="TRP1"
                     /regulatory_class="promoter"
     misc_feature    607..717
                     /note="NubG; N-terminal moiety of ubiquitin (ubi4)"
     misc_difference 643..645
                     /label=mutation Ile to Gly
                     /note="mutation Ile to Gly"
     misc_feature    718..728
                     /label=linker
                     /note="linker"
     protein_bind    727..851
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1283..1585)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(1933..2589)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2590..2692)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(2870..2994)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3008..3097
                     /label=3xHA
                     /note="three tandem HA epitope tags"
     misc_feature    3098..3100
                     /label=stop codon
                     /note="stop codon"
     misc_feature    complement(3616..4778)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     promoter        complement(5292..5393)
                     /label=TRP1 promoter
     promoter        5499..5603
                     /label=AmpR promoter
     CDS             5604..6461
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6635..7223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"