Basic Vector Information
- Vector Name:
- NX33_GW
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7405 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Lalonde S, Sero A, Pratelli R, Pilot G, Chen J, Sardi MI, Parsa SA, Kim DY, Acharya BR, Stein EV, Hu HC, Villiers F, Takeda K, Yang Y, Han YS, Schwacke R, Chiang W, Kato N, Loque D, Assmann SM, Kwak J
NX33_GW vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
NX33_GW vector Sequence
LOCUS 40924_2274 7405 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector NX33_GW, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7405)
AUTHORS Lalonde S, Sero A, Pratelli R, Pilot G, Chen J, Sardi MI, Parsa SA,
Kim DY, Acharya BR, Stein EV, Hu HC, Villiers F, Takeda K, Yang Y,
Han YS, Schwacke R, Chiang W, Kato N, Loque D, Assmann SM, Kwak JM,
Schroeder JI, Rhee SY, Frommer WB.
TITLE A Membrane Protein/Signaling Protein Interaction Network for
Arabidopsis Version AMPv2
JOURNAL Front Physiol 1, 24 (2010)
PUBMED 21423366
REFERENCE 2 (bases 1 to 7405)
AUTHORS Lalonde S, Frommer WB.
TITLE Direct Submission
JOURNAL Submitted (10-DEC-2010) Plant Biology, Carnegie Institution, 260
Panama St, Stanford, CA 94305, USA
REFERENCE 3 (bases 1 to 7405)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7405)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Front
Physiol 1, 24 (2010)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(10-DEC-2010) Plant Biology, Carnegie Institution, 260 Panama St,
Stanford, CA 94305, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7405
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 106..127
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 142..172
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 180..196
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 204..220
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
regulatory 245..606
/label=TRP1
/note="TRP1"
/regulatory_class="promoter"
misc_feature 607..717
/note="NubG; N-terminal moiety of ubiquitin (ubi4)"
misc_difference 643..645
/label=mutation Ile to Gly
/note="mutation Ile to Gly"
misc_feature 718..728
/label=linker
/note="linker"
protein_bind 727..851
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(1283..1585)
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
CDS complement(1933..2589)
/label=CmR
/note="chloramphenicol acetyltransferase"
promoter complement(2590..2692)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
protein_bind complement(2870..2994)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
CDS 3008..3097
/label=3xHA
/note="three tandem HA epitope tags"
misc_feature 3098..3100
/label=stop codon
/note="stop codon"
misc_feature complement(3616..4778)
/label=CEN/ARS
/note="S. cerevisiae CEN4 centromere fused to the
autonomously replicating sequence ARS1/ARS416"
promoter complement(5292..5393)
/label=TRP1 promoter
promoter 5499..5603
/label=AmpR promoter
CDS 5604..6461
/label=AmpR
/note="beta-lactamase"
rep_origin 6635..7223
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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