LOCUS       40924_2274        7405 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector NX33_GW, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Lalonde S, Sero A, Pratelli R, Pilot G, Chen J, Sardi MI, Parsa SA, 
            Kim DY, Acharya BR, Stein EV, Hu HC, Villiers F, Takeda K, Yang Y, 
            Han YS, Schwacke R, Chiang W, Kato N, Loque D, Assmann SM, Kwak JM, 
            Schroeder JI, Rhee SY, Frommer WB.
  TITLE     A Membrane Protein/Signaling Protein Interaction Network for 
            Arabidopsis Version AMPv2
  JOURNAL   Front Physiol 1, 24 (2010)
  PUBMED    21423366
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   Lalonde S, Frommer WB.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2010) Plant Biology, Carnegie Institution, 260 
            Panama St, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 7405)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7405)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Physiol 1, 24 (2010)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2010) Plant Biology, Carnegie Institution, 260 Panama St, 
            Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      245..606
                     /label=TRP1
                     /note="TRP1"
                     /regulatory_class="promoter"
     misc_feature    607..717
                     /note="NubG; N-terminal moiety of ubiquitin (ubi4)"
     misc_difference 643..645
                     /label=mutation Ile to Gly
                     /note="mutation Ile to Gly"
     misc_feature    718..728
                     /label=linker
                     /note="linker"
     protein_bind    727..851
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1283..1585)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(1933..2589)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2590..2692)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(2870..2994)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3008..3097
                     /label=3xHA
                     /note="three tandem HA epitope tags"
     misc_feature    3098..3100
                     /label=stop codon
                     /note="stop codon"
     misc_feature    complement(3616..4778)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     promoter        complement(5292..5393)
                     /label=TRP1 promoter
     promoter        5499..5603
                     /label=AmpR promoter
     CDS             5604..6461
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6635..7223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcgttgtc tcaccatatc cgcaatgaca aaaaaaatga tggaagacac taaaggaaaa
      301 aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg aggggtatct
      361 tcgaacacac gaaacttttt ccttccttca ttcacgcaca ctactctcta atgagcaacg
      421 gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaaagtttg ccgctttgct
      481 atcaagtata aatagacctg caattattaa tcttttgttt cctcgtcatt gttctcgttc
      541 cctttcttcc ttgtttcttt ttctgcacaa tatttcaagc tataccaagc atacaatcaa
      601 ctcgagatgc agattttcgt caagactttg accggtaaaa ccggaacatt ggaagttgaa
      661 tcttccgata ccatcgacaa cgttaagtcg aaaattcaag acaaggaagg aatccctggt
      721 gggtcgacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
      781 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
      841 cagtcactat gaatcaacta cttagatggt attagtgacc tgtagtcgac cgacagcctt
      901 ccaaatgttc ttcgggtgat gctgccaact tagtcgaccg acagccttcc aaatgttctt
      961 ctcaaacgga atcgtcgtat ccagcctact cgctattgtc ctcaatgccg tattaaatca
     1021 taaaaagaaa taagaaaaag aggtgcgagc ctcttttttg tgtgacaaaa taaaaacatc
     1081 tacctattca tatacgctag tgtcatagtc ctgaaaatca tctgcatcaa gaacaatttc
     1141 acaactctta tacttttctc ttacaagtcg ttcggcttca tctggatttt cagcctctat
     1201 acttactaaa cgtgataaag tttctgtaat ttctactgta tcgacctgca gactggctgt
     1261 gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt
     1321 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg
     1381 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
     1441 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
     1501 ccggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
     1561 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg
     1621 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
     1681 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
     1741 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
     1801 gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat
     1861 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
     1921 acgcggcgtt tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     1981 tctgccgaca tggaagccat cacaracggc atgatgaacc tgaatcgcca gcggcatcag
     2041 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     2101 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     2161 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     2221 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     2281 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     2341 caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc
     2401 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     2461 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     2521 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     2581 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     2641 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     2701 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
     2761 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc
     2821 tgccaactta gtcgactaca ggtcactaat accatctaag tagttgattc atagtgactg
     2881 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     2941 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttggtgg
     3001 tggcggatac ccatacgatg ttcctgacta tgcgggctat ccgtatgacg tcccggacta
     3061 tgcaggatcc tatccatatg acgttccaga ttacgcttga actagttggt gtggaagtcc
     3121 atatatcggt gatcaatata gtggttgaca tgctggctag tcaacattga gccttttgat
     3181 catgcaaata tattacggta ttttacaatc aaatatcaaa cttaactatt gactttataa
     3241 cttatttagg tggtaacatt cttataaaaa agaaaaaaat tactgcaaaa cagtactagc
     3301 ttttaacttg tatcctaggt tatctatgct gtctcaccat agagaatatt acctatttca
     3361 gaatgtatgt ccatgattcg ccgggtaaat acatataata cacaaatctg gcttaataaa
     3421 gtctataata tatctcataa agaagtgcta aattggctag tgctatatat ttttaagaaa
     3481 atttcttttg actaagtcca tatcgacttt gtaaaagttc actttagcat acatatatta
     3541 cacgagccag aaattgtaac ttttgcctaa aatcacaaat tgcaaaattt aattgcttgc
     3601 aaaaggtcac atgcttataa tcaacttttt taaaaattta aaatactttt ttatttttta
     3661 tttttaaaca taaatgaaat aatttattta ttgtttatga ttaccgaaac ataaaacctg
     3721 ctcaagaaaa agaaactgtt ttgtccttgg aaaaaaagca ctacctagga gcggccaaaa
     3781 tgccgaggct ttcatagctt aaactcttta cagaaaatag gcattataga tcagttcgag
     3841 ttttcttatt cttccttccg gttttatcgt cacagtttta cagtaaataa gtatcacctc
     3901 ttagagttcg atgataagct gtcaaacatg agaattaatt ccacatgtta aaatagtgaa
     3961 ggagcatgtt cggcacacag tggaccgaac gtggggtaag tgcactaggg tccggttaaa
     4021 cggatctcgc attgatgagg caacgctaat tatcaacata tagattgtta tctatctgca
     4081 tgaacacgaa atctttactt gacgacttga ggctgatggt gtttatgcaa agaaaccact
     4141 gtgtttaata tgtgtcactg tttgatatta ctgtcagcgt agaagataat agtaaaagcg
     4201 gttaataagt gtatttgaga taagtgtgat aaagttttta cagcgaaaag acgataaata
     4261 caagaaaatg attacgagga tacggagaga ggtatgtaca tgtgtattta tatactaagc
     4321 tgccggcggt tgtttgcaag accgagaaaa ggctagcaag aatcgggtca ttgtagcgta
     4381 tgcgcctgtg aacattctct tcaacaagtt tgattccatt gcggtgaaat ggtaaaagtc
     4441 aaccccctgc gatgtatatt ttcctgtaca atcaatcaaa aagccaaatg atttagcatt
     4501 atctttacat cttgttattt tacagatttt atgtttagat cttttatgct tgcttttcaa
     4561 aaggcttgca ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat
     4621 ttcttagcat ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc
     4681 acacctccgc ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt
     4741 tctggcgtca gtccaccagc taacataaaa tgtaagctct cggggctctc ttgccttcca
     4801 acccagtcag aaatcgagtt ccaatccaaa agttcacctg tcccacctgc ttctgaatca
     4861 aacaagggaa taaacgaatg aggtttctgt gaagctgcac tgagtagtat gttgcagtct
     4921 tttggaaata cgagtctttt aataactggc aaaccgagga actcttggta ttcttgccac
     4981 gactcatctc catgcagttg gacgatatca atgccgtaat cattgaccag agccaaaaca
     5041 tcctccttag gttgattacg aaacacgcca accaagtatt tcggagtgcc tgaactattt
     5101 ttatatgctt ttacaagact tgaaattttc cttgcaataa ccgggtcaat tgttctcttt
     5161 ctattgggca cacatataat acccagcaag tcagcatcgg aatctagtgc acattctgcg
     5221 gcctctgtgc tctgcaagcc gcaaactttc accaatggac cagaactacc tgtgaaatta
     5281 ataacagaca tactccaagc tgcctttgtg tgcttaatca cgtatactca cgtgctcaat
     5341 agtcaccaat gccctccctc ttggccctct ccttttcttt tttcgaccga attaattctt
     5401 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5461 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5521 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5581 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5641 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5701 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5761 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5821 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5881 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5941 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6001 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6061 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6121 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6181 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6241 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6301 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6361 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6421 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6481 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6541 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6601 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6661 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6721 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6781 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6841 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6901 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6961 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7021 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7081 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7141 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7201 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7261 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7321 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7381 agtcagtgag cgaggaagcg gaaga
//
