NgoMIV vector (V009835)

MSDS

Basic Vector Information

Vector Name:: NgoMIV

Antibiotic Resistance:: Ampicillin

Length:: 3236 bp

Type:: Shuttle vector

Replication origin:: ori

Source/Author:: Veltman DM, Akar G, Bosgraaf L, van Haastert PJM.

NgoMIV vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

NgoMIV vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_2209        3236 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector NgoMIV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, van Haastert PJM.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2008) Cell Biochemistry, Rijks Universiteit 
            Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands
REFERENCE   2  (bases 1 to 3236)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3236)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (20-OCT-2008) Cell Biochemistry, Rijks Universiteit Groningen, 
            Kerklaan 30, Haren 9751 NN, The Netherlands"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..290
                     /label=act15
                     /note="act15"
                     /regulatory_class="promoter"
     regulatory      320..581
                     /label=act8
                     /note="act8"
                     /regulatory_class="terminator"
     primer_bind     complement(601..617)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1091..1195
                     /label=AmpR promoter
     CDS             1196..2053
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2227..2815
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3103..3124
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3139..3169
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3177..3193
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3201..3217
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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