NgoMIV vector (V009835)

Basic Vector Information

      • Vector Name:
      • NgoMIV
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3236 bp
      • Type:
      • Shuttle vector
      • Source/Author:
      • Veltman DM, Akar G, Bosgraaf L, van Haastert PJM.

NgoMIV vector Vector Map

NgoMIV3236 bp6001200180024003000act15act8M13 fwdAmpR promoterpenicillin beta-lactamasepUC oriCAP binding sitelac promoterlac operatorM13 rev

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

NgoMIV vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                3236 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector NgoMIV, complete sequence.
ACCESSION   FJ402941
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, van Haastert PJM.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2008) Cell Biochemistry, Rijks Universiteit 
            Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Shuttle vector NgoMIV"
                     /mol_type="other DNA"
                     /db_xref="taxon:570119"
     regulatory      7..290
                     /regulatory_class="promoter"
                     /note="act15"
     regulatory      320..581
                     /regulatory_class="terminator"
                     /note="act8"
     primer_bind     complement(601..617)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1091..1195
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1196..2056
                     /codon_start=1
                     /product="penicillin beta-lactamase"
                     /label=penicillin beta-lactamase
                     /protein_id="ACJ38093.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2204..2871
                     /label=pUC ori
                     /note="pUC ori"
     rep_origin      2227..2815
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3103..3124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3139..3169
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3177..3193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3201..3217
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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