LOCUS       40924_2209        3236 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector NgoMIV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, van Haastert PJM.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2008) Cell Biochemistry, Rijks Universiteit 
            Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands
REFERENCE   2  (bases 1 to 3236)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3236)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (20-OCT-2008) Cell Biochemistry, Rijks Universiteit Groningen, 
            Kerklaan 30, Haren 9751 NN, The Netherlands"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..290
                     /label=act15
                     /note="act15"
                     /regulatory_class="promoter"
     regulatory      320..581
                     /label=act8
                     /note="act8"
                     /regulatory_class="terminator"
     primer_bind     complement(601..617)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1091..1195
                     /label=AmpR promoter
     CDS             1196..2053
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2227..2815
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3103..3124
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3139..3169
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3177..3193
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3201..3217
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gccggctaaa aaaaattttt atttattttt atttattttg aattaaatag attacaaatt
       61 aattaatccc atcaaatctt taaaaaaaaa tggtttaaaa aaacttgggt tggttaatta
      121 ttatttgaaa attttaaaac ccaaattaaa aaaaaaaaat gggattcaaa aatttttttt
      181 tttttttttt tttttttttt tttttttttt tcagattgca taaaaagatt tttttttttt
      241 ttttttctta tttcttaaaa caaataaatt aaattaaata aaaaataaaa atcagatcta
      301 gtactagtta aataaataaa ttatttaata aataataaaa aaacaaattg ttgtaataat
      361 ctaatatttt cttttttttt taattttttt tttttaaatc ttaataatta ttaagttatt
      421 ttaatttttt tttttttttt tttttttttt tttttttttt ttctatcaaa aaaatcaaat
      481 atatttaaaa aatttattat ttacagatac attttgaatg gtgaagataa atatatgcat
      541 tagatgtaaa acagccaaag agtatgaaaa tcaaaaagat agccggccat caagcttggc
      601 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      661 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      721 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
      781 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
      841 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      901 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
      961 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     1021 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     1081 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1141 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1201 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1261 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1321 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1381 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     1441 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1501 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1561 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1621 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1681 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1741 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1801 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1861 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1921 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1981 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2041 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2101 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2161 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2221 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2281 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2341 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2401 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2461 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2521 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2581 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2641 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2701 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2761 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2821 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2881 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2941 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3001 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     3061 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     3121 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     3181 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat tcgatg
//
