LOCUS 40924_2209 3236 bp DNA circular SYN 18-DEC-2018 DEFINITION Shuttle vector NgoMIV, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3236) AUTHORS Veltman DM, Akar G, Bosgraaf L, van Haastert PJM. TITLE Direct Submission JOURNAL Submitted (20-OCT-2008) Cell Biochemistry, Rijks Universiteit Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands REFERENCE 2 (bases 1 to 3236) TITLE Direct Submission REFERENCE 3 (bases 1 to 3236) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (20-OCT-2008) Cell Biochemistry, Rijks Universiteit Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..3236 /mol_type="other DNA" /organism="synthetic DNA construct" regulatory 7..290 /label=act15 /note="act15" /regulatory_class="promoter" regulatory 320..581 /label=act8 /note="act8" /regulatory_class="terminator" primer_bind complement(601..617) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 1091..1195 /label=AmpR promoter CDS 1196..2053 /label=AmpR /note="beta-lactamase" rep_origin 2227..2815 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 3103..3124 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 3139..3169 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3177..3193 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3201..3217 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gccggctaaa aaaaattttt atttattttt atttattttg aattaaatag attacaaatt 61 aattaatccc atcaaatctt taaaaaaaaa tggtttaaaa aaacttgggt tggttaatta 121 ttatttgaaa attttaaaac ccaaattaaa aaaaaaaaat gggattcaaa aatttttttt 181 tttttttttt tttttttttt tttttttttt tcagattgca taaaaagatt tttttttttt 241 ttttttctta tttcttaaaa caaataaatt aaattaaata aaaaataaaa atcagatcta 301 gtactagtta aataaataaa ttatttaata aataataaaa aaacaaattg ttgtaataat 361 ctaatatttt cttttttttt taattttttt tttttaaatc ttaataatta ttaagttatt 421 ttaatttttt tttttttttt tttttttttt tttttttttt ttctatcaaa aaaatcaaat 481 atatttaaaa aatttattat ttacagatac attttgaatg gtgaagataa atatatgcat 541 tagatgtaaa acagccaaag agtatgaaaa tcaaaaagat agccggccat caagcttggc 601 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 661 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 721 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct 781 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga 841 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc 901 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 961 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct 1021 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg 1081 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 1141 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 1201 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 1261 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 1321 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 1381 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 1441 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 1501 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 1561 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 1621 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 1681 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 1741 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 1801 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 1861 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 1921 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 1981 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 2041 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 2101 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 2161 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 2221 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 2281 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 2341 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca 2401 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 2461 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 2521 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 2581 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 2641 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 2701 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 2761 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 2821 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 2881 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 2941 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 3001 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 3061 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac 3121 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt 3181 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat tcgatg //