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mTol2-B-P-Gal4VP16-Cre-CG3 vector (V009840#)

Basic Vector Information

Basic Vector Information
Vector Name mTol2-B-P-Gal4VP16-Cre-CG3 Antibiotic Resistance Ampicillin
Length 9078 bp Type Expression vector
Source Otsuna H, Scoresby AN, Gaynes B, Tong ZZ, Kwan KK, Hutcheson DA, Chien CB, Dorsky R.

mTol2-B-P-Gal4VP16-Cre-CG3 vector Vector Map

T7 primer M13F LacZ alpha f1 ori AmpR promoter AmpR ColE1 origin CAP binding site lac promoter LacO M13R(1) SP6 miniTol2 3' end M13R attB4 adenovirus E1B TATA 5'UTR FRT(1) attB1 Gal4 DBD VP16 activation domain attB2 T7 promoter SV40 late polyA (from pCS2+)(1) from pBS302 FRT iCre attB3 M13 fwd SV40 late polyA from pCS2+ SV40 late polyA from pCS2+(2) T7 promoter EGFP cmlc2 promoter miniTol2 5' end T7 primer(1)

mTol2-B-P-Gal4VP16-Cre-CG3 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                9078 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector mTol2-B-P-Gal4VP16-Cre-CG3, complete sequence.
ACCESSION   KC800700
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Otsuna H, Scoresby AN, Gaynes B, Tong ZZ, Kwan KK, Hutcheson DA, 
            Chien CB, Dorsky R.
  TITLE     Large Scale Zebrafish Gal4 Enhancer Trap Screen for Neural Specific 
            Expression Patterns
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9078)
  AUTHORS   Otsuna H, Scoresby AN, Gaynes B, Tong ZZ, Kwan KK, Hutcheson DA, 
            Chien CB, Dorsky R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2013) Neurobiology and Anatomy, University of 
            Utah, 20 North 1900 East, Salt Lake City, UT 84132, USA
REFERENCE   3  (bases 1 to 9078)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Expression vector mTol2-B-P-Gal4VP16-Cre-CG3"
                     /mol_type="other DNA"
                     /db_xref="taxon:1344359"
     primer_bind     complement(1..18)
                     /label=T7 primer
                     /note="T7 primer"
     primer_bind     complement(24..41)
                     /label=M13F
                     /note="M13F"
     primer_bind     complement(24..40)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    112..180
                     /label=LacZ alpha
                     /note="LacZ alpha"
     rep_origin      181..636
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      198..504
                     /label=f1(+) ori
                     /note="f1(+) ori"
     promoter        714..818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             819..1679
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1017..1679
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="AGN90802.1"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     rep_origin      1774..2456
                     /label=ColE1 origin
                     /note="ColE1 origin"
     rep_origin      1850..2438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2726..2747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2762..2792
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_feature    2796..2818
                     /label=LacO
                     /note="LacO"
     protein_bind    2800..2816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2824..2844
                     /label=M13R(1)
                     /note="M13R(1)"
     primer_bind     2824..2840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2850..2875
                     /label=SP6
                     /note="SP6"
     promoter        2858..2876
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     repeat_region   2927..3085
                     /label=miniTol2 3' end
                     /note="miniTol2 3' end"
     repeat_region   2927..2943
                     /label=Tol2 3' TIR
                     /note="Tol2 3' TIR"
     primer_bind     3108..3129
                     /label=M13R
                     /note="M13R"
     primer_bind     3108..3124
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3144..3164
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_recomb     3144..3150
                     /label=attR4
                     /note="attR4"
     misc_feature    3144..3150
                     /label=att4_shared(1)
                     /note="att4_shared(1)"
     misc_recomb     3151..3164
                     /label=attL4
                     /note="attL4"
     misc_feature    3151..3164
                     /label=att4_shared
                     /note="att4_shared"
     regulatory      3193..3203
                     /regulatory_class="promoter"
                     /note="adenovirus E1B TATA"
     5'UTR           3211..3242
                     /label=carp beta-actin trx start
                     /note="carp beta-actin trx start"
     misc_feature    3249..3282
                     /label=FRT(1)
                     /note="FRT(1)"
     protein_bind    3249..3282
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    3283..3307
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     3284..3290
                     /label=attR1
                     /note="attR1"
     misc_feature    3288..3290
                     /label=att1_shared
                     /note="att1_shared"
     misc_recomb     3291..3307
                     /label=attL1
                     /note="attL1"
     misc_feature    3291..3301
                     /label=att1_shared(1)
                     /note="att1_shared(1)"
     misc_feature    3313..3752
                     /label=Gal4 DBD
                     /note="Gal4 DBD"
     CDS             3313..3750
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTV"
     misc_feature    3790..3963
                     /label=VP16 activation domain
                     /note="VP16 activation domain"
     protein_bind    complement(3968..3992)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     3968..3977
                     /label=attL2
                     /note="attL2"
     misc_feature    3974..3977
                     /label=att2_shared
                     /note="att2_shared"
     misc_recomb     3978..3991
                     /label=attR2
                     /note="attR2"
     misc_feature    3978..3989
                     /label=att2_shared(1)
                     /note="att2_shared(1)"
     promoter        complement(3993..4010)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4015..4212
                     /label=SV40 late polyA (from pCS2+)(1)
                     /note="SV40 late polyA (from pCS2+)(1)"
     polyA_signal    4015..4149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    4249..5597
                     /label=from pBS302
                     /note="from pBS302"
     misc_feature    complement(4261..4699)
                     /label=yeast ded1/HIS3 protein fragment X03245.1
                     /note="yeast ded1/HIS3 protein fragment X03245.1"
     regulatory      4699..5597
                     /regulatory_class="other"
                     /note="STOP sequence"
     regulatory      4700..5556
                     /regulatory_class="other"
                     /note="STOP sequence from SV40"
     misc_feature    4706..5319
                     /label=SV40 4713..4100
                     /note="SV40 4713..4100"
     intron          4706..4777
                     /note="SV40 small T antigen intron"
     intron          4782..4847
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4977..4997
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5316..5556
                     /label=SV40 2774..2534
                     /note="SV40 2774..2534"
     misc_feature    complement(5359..5556)
                     /label=SV40 late polyA (from pCS2+)(3)
                     /note="SV40 late polyA (from pCS2+)(3)"
     polyA_signal    5422..5556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     5598..5631
                     /label=FRT
                     /note="FRT"
     misc_feature    5598..5631
                     /label=FRT
                     /note="FRT"
     protein_bind    5598..5631
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    5645..6700
                     /label=iCre
                     /note="iCre"
     CDS             5651..5671
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6712..6732)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_recomb     6712..6718
                     /label=attL3
                     /note="attL3"
     misc_feature    6712..6718
                     /label=att3_shared
                     /note="att3_shared"
     misc_recomb     6719..6732
                     /label=attR3
                     /note="attR3"
     misc_feature    6719..6726
                     /label=att3_shared(1)
                     /note="att3_shared(1)"
     primer_bind     complement(6740..6756)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6741..6756)
                     /label=M13F(1)
                     /note="M13F(1)"
     misc_feature    6772..6969
                     /label=SV40 late polyA from pCS2+
                     /note="SV40 late polyA from pCS2+"
     polyA_signal    6772..6906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(7028..7160)
                     /label=SV40 late polyA from pCS2+(2)
                     /note="SV40 late polyA from pCS2+(2)"
     promoter        7165..7182
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(7208..7927)
                     /codon_start=1
                     /product="EGFP"
                     /label=EGFP
                     /protein_id="AGN90801.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    complement(7723..7736)
                     /label=EGFP-like chromophore
                     /note="EGFP-like chromophore"
     regulatory      complement(7928..8826)
                     /regulatory_class="promoter"
                     /note="cmlc2 promoter"
     repeat_region   8850..9064
                     /label=miniTol2 5' end
                     /note="miniTol2 5' end"
     repeat_region   9046..9064
                     /label=Tol2 5' TIR
                     /note="Tol2 5' TIR"
     primer_bind     complement(9074..9078)
                     /label=T7 primer(1)
                     /note="T7 primer(1)"
     promoter        complement(9077..17)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"

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